Entomotoxic efficacy of fungus-synthesized nanoparticles against immature stages of stored bean pests
Abstract Nanopesticides, particularly biosynthesized ones using organic reductants, hold great promise as a cost-effective and eco-friendly alternative to chemical pesticides. However, their efficacy on stored product pests, which can cause damage to dried grains, has not been extensively tested, es...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-35697-1 |
| _version_ | 1797817995772297216 |
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| author | Eman Ahmed Mohamed Helmy Phyu Phyu San Yao Zhuo Zhang Charles Adarkwah Midori Tuda |
| author_facet | Eman Ahmed Mohamed Helmy Phyu Phyu San Yao Zhuo Zhang Charles Adarkwah Midori Tuda |
| author_sort | Eman Ahmed Mohamed Helmy |
| collection | DOAJ |
| description | Abstract Nanopesticides, particularly biosynthesized ones using organic reductants, hold great promise as a cost-effective and eco-friendly alternative to chemical pesticides. However, their efficacy on stored product pests, which can cause damage to dried grains, has not been extensively tested, especially on immature stages. Here, we biosynthesized six types of nanoparticles (NPs) using extracts from the fungus Fusarium solani: silver (AgNPs), selenium (SeNPs), silicon dioxide (SiO2NPs), copper oxide (CuONPs), titanium dioxide (TiO2NPs) and zinc oxide (ZnONPs) ranging in size from 8 to 33 nm. To test their efficacy on stored bean pests, they were applied to the eggs and larvae of pest beetles Callosobruchus chinensis and Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae), which burrow into seeds as larvae. Susceptibility to the NPs was species-dependent and differed between developmental stages; eggs were more susceptible than larvae inhabiting in seeds. SeNPs and TiO2NPs reduced the hatchability of C. chinensis eggs by 23% and 18% compared to the control, respectively, leading to an 18% reduction in egg-to-adult survival by SeNPs. In C. maculatus, TiO2NPs applied to eggs reduced larva-to-adult survivorship by 11%, resulting in a 15% reduction in egg-to-adult survival. The egg mass of C. chinensis was 23% smaller than that of C. maculatus: the higher surface-area-to-volume ratio of the C. chinensis eggs could explain their higher acute mortality caused by the NPs compared to C. maculatus eggs. The biosynthesized SeNPs and TiO2NPs have potential for controlling major stored bean pests when applied to their eggs. This is the first to show the efficacy of biosynthesized SeNPs and TiO2NPs on stored product pests and the efficacy of Fusarium-synthesized NPs on insects. |
| first_indexed | 2024-03-13T09:01:48Z |
| format | Article |
| id | doaj.art-9670b1be266e48109cd357018968da2d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T09:01:48Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9670b1be266e48109cd357018968da2d2023-05-28T11:17:35ZengNature PortfolioScientific Reports2045-23222023-05-011311810.1038/s41598-023-35697-1Entomotoxic efficacy of fungus-synthesized nanoparticles against immature stages of stored bean pestsEman Ahmed Mohamed Helmy0Phyu Phyu San1Yao Zhuo Zhang2Charles Adarkwah3Midori Tuda4The Regional Centre for Mycology and Biotechnology (RCMB), Al-Azhar UniversityLaboratory of Insect Natural Enemies, Institute of Biological Control, Faculty of Agriculture, Kyushu UniversityLaboratory of Insect Natural Enemies, Institute of Biological Control, Faculty of Agriculture, Kyushu UniversityLaboratory of Insect Natural Enemies, Institute of Biological Control, Faculty of Agriculture, Kyushu UniversityLaboratory of Insect Natural Enemies, Institute of Biological Control, Faculty of Agriculture, Kyushu UniversityAbstract Nanopesticides, particularly biosynthesized ones using organic reductants, hold great promise as a cost-effective and eco-friendly alternative to chemical pesticides. However, their efficacy on stored product pests, which can cause damage to dried grains, has not been extensively tested, especially on immature stages. Here, we biosynthesized six types of nanoparticles (NPs) using extracts from the fungus Fusarium solani: silver (AgNPs), selenium (SeNPs), silicon dioxide (SiO2NPs), copper oxide (CuONPs), titanium dioxide (TiO2NPs) and zinc oxide (ZnONPs) ranging in size from 8 to 33 nm. To test their efficacy on stored bean pests, they were applied to the eggs and larvae of pest beetles Callosobruchus chinensis and Callosobruchus maculatus (Coleoptera: Chrysomelidae: Bruchinae), which burrow into seeds as larvae. Susceptibility to the NPs was species-dependent and differed between developmental stages; eggs were more susceptible than larvae inhabiting in seeds. SeNPs and TiO2NPs reduced the hatchability of C. chinensis eggs by 23% and 18% compared to the control, respectively, leading to an 18% reduction in egg-to-adult survival by SeNPs. In C. maculatus, TiO2NPs applied to eggs reduced larva-to-adult survivorship by 11%, resulting in a 15% reduction in egg-to-adult survival. The egg mass of C. chinensis was 23% smaller than that of C. maculatus: the higher surface-area-to-volume ratio of the C. chinensis eggs could explain their higher acute mortality caused by the NPs compared to C. maculatus eggs. The biosynthesized SeNPs and TiO2NPs have potential for controlling major stored bean pests when applied to their eggs. This is the first to show the efficacy of biosynthesized SeNPs and TiO2NPs on stored product pests and the efficacy of Fusarium-synthesized NPs on insects.https://doi.org/10.1038/s41598-023-35697-1 |
| spellingShingle | Eman Ahmed Mohamed Helmy Phyu Phyu San Yao Zhuo Zhang Charles Adarkwah Midori Tuda Entomotoxic efficacy of fungus-synthesized nanoparticles against immature stages of stored bean pests Scientific Reports |
| title | Entomotoxic efficacy of fungus-synthesized nanoparticles against immature stages of stored bean pests |
| title_full | Entomotoxic efficacy of fungus-synthesized nanoparticles against immature stages of stored bean pests |
| title_fullStr | Entomotoxic efficacy of fungus-synthesized nanoparticles against immature stages of stored bean pests |
| title_full_unstemmed | Entomotoxic efficacy of fungus-synthesized nanoparticles against immature stages of stored bean pests |
| title_short | Entomotoxic efficacy of fungus-synthesized nanoparticles against immature stages of stored bean pests |
| title_sort | entomotoxic efficacy of fungus synthesized nanoparticles against immature stages of stored bean pests |
| url | https://doi.org/10.1038/s41598-023-35697-1 |
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