C-Terminal Extensions of Ku70 and Ku80 Differentially Influence DNA End Binding Properties

C-Terminal Extensions of Ku70 and Ku80 Differentially Influence DNA End Binding Properties

The Ku70/80 heterodimer binds to DNA ends and attracts other proteins involved in the non-homologous end-joining (NHEJ) pathway of DNA double-strand break repair. We developed a novel assay to measure DNA binding and release kinetics using differences in Förster resonance energy transfer (FRET) of t...

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Bibliographic Details
Main Authors: Takabumi Inagawa, Thomas Wennink, Joyce H. G. Lebbink, Guido Keijzers, Bogdan I. Florea, Nicole S. Verkaik, Dik C. van Gent
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:International Journal of Molecular Sciences
Subjects:
DNA repair
non-homologous end-joining
DNA-PK
DNA binding
surface plasmon resonance (SPR)
Online Access:https://www.mdpi.com/1422-0067/21/18/6725

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