Eicosanoid and cytokine levels differentiate between stages of MTB infection
Abstract Introduction: The need for biomarkers predicting the course of MTB infection and the necessity of specific therapy are well recognized. Recent data point to the role of cytokines and lipid mediators in protective immunity against tuberculosis. Aim:...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2023-06-01
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| Series: | Folia Medica |
| Online Access: | https://foliamedica.bg/article/80599/download/pdf/ |
| _version_ | 1797789996897271808 |
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| author | Yana Todorova Radoslava Emilova Vladimir Milanov Elizabeta Bachiyska Yuliana Atanasova Ana Baikova Maria Nikolova |
| author_facet | Yana Todorova Radoslava Emilova Vladimir Milanov Elizabeta Bachiyska Yuliana Atanasova Ana Baikova Maria Nikolova |
| author_sort | Yana Todorova |
| collection | DOAJ |
| description | Abstract Introduction: The need for biomarkers predicting the course of MTB infection and the necessity of specific therapy are well recognized. Recent data point to the role of cytokines and lipid mediators in protective immunity against tuberculosis. Aim: We evaluated the balance between cytokines, and eikosanoids as a possible prognostic indicator in MTB infection. Material and methods: The induced expression of effector and regulatory cytokines IFN-γ, TNF-α, IL-2, IL-17, IL-6, and IL-10 was measured in relation to the lipid mediators PGE2 and LXA4 in active TB infection (ATB, n=15) before and after therapy (ATB-T, n=6), established latent infection (LTBI, n=22), recent contacts of ATB (RC, n=12), and healthy controls (n=11) A flow cytometry microarray (CBA, BD Biosciences) and quantitative ELISA (SunRed Tech) were employed. Results: The regulatory cytokines (RC) were characterized by a high potential for IL-17 and Th1 cytokine secretion, combined with low IL-6 expression, while ATB donors had a partially preserved TNF-α potential, and higher IL-6 expression. The PGE2-to-LXA4 ratio discriminated between situations with high bacterial load (ATB), and contained infection (LTBI, ATB-T), and defined clearly cut subgroups among RC and ATB donors. Conclusions: Our results suggest that increased PGE2/LXA4 ratio coupled with high induced IL-10 level indicates infection after a recent contact. In the settings of ATB, increased ratio and low TNF-α level point to inefficient granuloma formation in the settings of ATB. |
| first_indexed | 2024-03-13T01:58:29Z |
| format | Article |
| id | doaj.art-96725e53de054960acd64700c996a999 |
| institution | Directory Open Access Journal |
| issn | 1314-2143 |
| language | English |
| last_indexed | 2024-03-13T01:58:29Z |
| publishDate | 2023-06-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Folia Medica |
| spelling | doaj.art-96725e53de054960acd64700c996a9992023-07-02T08:11:14ZengPensoft PublishersFolia Medica1314-21432023-06-0165339940610.3897/folmed.65.e8059980599Eicosanoid and cytokine levels differentiate between stages of MTB infectionYana Todorova0Radoslava Emilova1Vladimir Milanov2Elizabeta Bachiyska3Yuliana Atanasova4Ana Baikova5Maria Nikolova6National Center of Infectious and Parasitic DiseasesNational Center of Infectious and Parasitic DiseasesSt Sofia University Hospital of Pulmonary DiseasesNational Center of Infectious and Parasitic DiseasesNational Center of Infectious and Parasitic DiseasesNational Center of Infectious and Parasitic DiseasesNational Center of Infectious and Parasitic DiseasesAbstract Introduction: The need for biomarkers predicting the course of MTB infection and the necessity of specific therapy are well recognized. Recent data point to the role of cytokines and lipid mediators in protective immunity against tuberculosis. Aim: We evaluated the balance between cytokines, and eikosanoids as a possible prognostic indicator in MTB infection. Material and methods: The induced expression of effector and regulatory cytokines IFN-γ, TNF-α, IL-2, IL-17, IL-6, and IL-10 was measured in relation to the lipid mediators PGE2 and LXA4 in active TB infection (ATB, n=15) before and after therapy (ATB-T, n=6), established latent infection (LTBI, n=22), recent contacts of ATB (RC, n=12), and healthy controls (n=11) A flow cytometry microarray (CBA, BD Biosciences) and quantitative ELISA (SunRed Tech) were employed. Results: The regulatory cytokines (RC) were characterized by a high potential for IL-17 and Th1 cytokine secretion, combined with low IL-6 expression, while ATB donors had a partially preserved TNF-α potential, and higher IL-6 expression. The PGE2-to-LXA4 ratio discriminated between situations with high bacterial load (ATB), and contained infection (LTBI, ATB-T), and defined clearly cut subgroups among RC and ATB donors. Conclusions: Our results suggest that increased PGE2/LXA4 ratio coupled with high induced IL-10 level indicates infection after a recent contact. In the settings of ATB, increased ratio and low TNF-α level point to inefficient granuloma formation in the settings of ATB.https://foliamedica.bg/article/80599/download/pdf/ |
| spellingShingle | Yana Todorova Radoslava Emilova Vladimir Milanov Elizabeta Bachiyska Yuliana Atanasova Ana Baikova Maria Nikolova Eicosanoid and cytokine levels differentiate between stages of MTB infection Folia Medica |
| title | Eicosanoid and cytokine levels differentiate between stages of MTB infection |
| title_full | Eicosanoid and cytokine levels differentiate between stages of MTB infection |
| title_fullStr | Eicosanoid and cytokine levels differentiate between stages of MTB infection |
| title_full_unstemmed | Eicosanoid and cytokine levels differentiate between stages of MTB infection |
| title_short | Eicosanoid and cytokine levels differentiate between stages of MTB infection |
| title_sort | eicosanoid and cytokine levels differentiate between stages of mtb infection |
| url | https://foliamedica.bg/article/80599/download/pdf/ |
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