MIC-1 Antlerogenic Stem Cells Homogenate from <i>Cervus elaphus</i> Accelerate Corneal Burn Reepithelization in Rabbits
Deer antler is the only mammalian organ that can fully grow back once lost from its pedicle. Antler regeneration is a stem cell-based process. Therefore, antlers probably offer the most pertinent model for studying organ regeneration in mammals. Evaluation of the effect of deer antler stem cells on...
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MDPI AG
2022-02-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/12/5/2468 |
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| author | Wojciech Dziewiszek Marek Bochnia Dorota Szumny Stanisław Dzimira Adam Szeląg Antoni Szumny |
| author_facet | Wojciech Dziewiszek Marek Bochnia Dorota Szumny Stanisław Dzimira Adam Szeląg Antoni Szumny |
| author_sort | Wojciech Dziewiszek |
| collection | DOAJ |
| description | Deer antler is the only mammalian organ that can fully grow back once lost from its pedicle. Antler regeneration is a stem cell-based process. Therefore, antlers probably offer the most pertinent model for studying organ regeneration in mammals. Evaluation of the effect of deer antler stem cells on the healing of superficial and deep rabbit corneal wounds was performed. Thirty-six New Zealeand White rabbits were used in this study in superficial and deep denaturation models, and corneal erosion was performed with <i>n</i>-heptanol placed on the cornea for 30 and NaOH for 90 s. Antler stem cells in drop formulation with hyaluronate was used. As a control, sodium hyaluronate in the superficial model and protein-free calf blood dialysate (Solcoseryl) in the deep model were administered. In superficial corneal damage, a reduction in the area of the damaged cornea was observed from day 3 of the experiment to an adequate level: 45% in the test group and 52% in the control group relative to the baseline damage (100%). Between days 3 and 7, on average, a smaller lesion area was observed in the group receiving antler stem cells. The use of antler stem cells has resulted in a marked improvement in cornea clarity. According to the 5-point scale of corneal opacity evaluation, where 1 is completely clear and 5 is completely opaque, the first statistically significant changes were observed after 4 weeks of treatment: 3.0 in the study group, 4.1 in the control with Solcoseryl, and 4.4 in the control group. After 9 weeks, these values were, 2.5, 3.8, and 4.1, respectively. The present preliminary study shows the promising results of antlerogenic stem cells of <i>Cervus elaphus</i> topically applied for the treatment of corneal injury. A deeper understanding of the developmental mechanisms involved in antler renewal can be useful for controlling regeneration cornea processes. |
| first_indexed | 2024-03-09T20:47:49Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T20:47:49Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-9672c424b56643afaf4b78f7cd827d632023-11-23T22:41:16ZengMDPI AGApplied Sciences2076-34172022-02-01125246810.3390/app12052468MIC-1 Antlerogenic Stem Cells Homogenate from <i>Cervus elaphus</i> Accelerate Corneal Burn Reepithelization in RabbitsWojciech Dziewiszek0Marek Bochnia1Dorota Szumny2Stanisław Dzimira3Adam Szeląg4Antoni Szumny5Department of Pharmacology, Wroclaw Medical University, ul. Mikulicza-Radeckiego 2, 50-345 Wroclaw, PolandDepartment and Clinic of Otolaryngology, Head and Neck Surgery, Wroclaw Medical University, ul. Borowska 213, 50-556 Wrocław, PolandDepartment of Pharmacology, Wroclaw Medical University, ul. Mikulicza-Radeckiego 2, 50-345 Wroclaw, PolandDepartment of Pathology, Wroclaw University of Environmemntal and Life Sciences, C.K. Norwida 31, 50-375 Wroclaw, PolandDepartment of Pharmacology, Wroclaw Medical University, ul. Mikulicza-Radeckiego 2, 50-345 Wroclaw, PolandDepartment of Food Chemistry and Biocatalysis, Wroclaw University of Environmental and Life Sciences, C.K. Norwida 25, 50-375 Wroclaw, PolandDeer antler is the only mammalian organ that can fully grow back once lost from its pedicle. Antler regeneration is a stem cell-based process. Therefore, antlers probably offer the most pertinent model for studying organ regeneration in mammals. Evaluation of the effect of deer antler stem cells on the healing of superficial and deep rabbit corneal wounds was performed. Thirty-six New Zealeand White rabbits were used in this study in superficial and deep denaturation models, and corneal erosion was performed with <i>n</i>-heptanol placed on the cornea for 30 and NaOH for 90 s. Antler stem cells in drop formulation with hyaluronate was used. As a control, sodium hyaluronate in the superficial model and protein-free calf blood dialysate (Solcoseryl) in the deep model were administered. In superficial corneal damage, a reduction in the area of the damaged cornea was observed from day 3 of the experiment to an adequate level: 45% in the test group and 52% in the control group relative to the baseline damage (100%). Between days 3 and 7, on average, a smaller lesion area was observed in the group receiving antler stem cells. The use of antler stem cells has resulted in a marked improvement in cornea clarity. According to the 5-point scale of corneal opacity evaluation, where 1 is completely clear and 5 is completely opaque, the first statistically significant changes were observed after 4 weeks of treatment: 3.0 in the study group, 4.1 in the control with Solcoseryl, and 4.4 in the control group. After 9 weeks, these values were, 2.5, 3.8, and 4.1, respectively. The present preliminary study shows the promising results of antlerogenic stem cells of <i>Cervus elaphus</i> topically applied for the treatment of corneal injury. A deeper understanding of the developmental mechanisms involved in antler renewal can be useful for controlling regeneration cornea processes.https://www.mdpi.com/2076-3417/12/5/2468corneastem cellscorneal burn<i>Cervus elaphus</i>antler |
| spellingShingle | Wojciech Dziewiszek Marek Bochnia Dorota Szumny Stanisław Dzimira Adam Szeląg Antoni Szumny MIC-1 Antlerogenic Stem Cells Homogenate from <i>Cervus elaphus</i> Accelerate Corneal Burn Reepithelization in Rabbits Applied Sciences cornea stem cells corneal burn <i>Cervus elaphus</i> antler |
| title | MIC-1 Antlerogenic Stem Cells Homogenate from <i>Cervus elaphus</i> Accelerate Corneal Burn Reepithelization in Rabbits |
| title_full | MIC-1 Antlerogenic Stem Cells Homogenate from <i>Cervus elaphus</i> Accelerate Corneal Burn Reepithelization in Rabbits |
| title_fullStr | MIC-1 Antlerogenic Stem Cells Homogenate from <i>Cervus elaphus</i> Accelerate Corneal Burn Reepithelization in Rabbits |
| title_full_unstemmed | MIC-1 Antlerogenic Stem Cells Homogenate from <i>Cervus elaphus</i> Accelerate Corneal Burn Reepithelization in Rabbits |
| title_short | MIC-1 Antlerogenic Stem Cells Homogenate from <i>Cervus elaphus</i> Accelerate Corneal Burn Reepithelization in Rabbits |
| title_sort | mic 1 antlerogenic stem cells homogenate from i cervus elaphus i accelerate corneal burn reepithelization in rabbits |
| topic | cornea stem cells corneal burn <i>Cervus elaphus</i> antler |
| url | https://www.mdpi.com/2076-3417/12/5/2468 |
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