Effect of Esculetin on <i>Tert</i>-Butyl Hydroperoxide-Induced Oxidative Injury in Retinal Pigment Epithelial Cells In Vitro
Esculetin is a coumarin-derived compound with antioxidant and anti-inflammatory properties. The current study aims to evaluate the therapeutic implications of esculetin on retinal dysfunction and uncover the underlying mechanisms. <i>Tert</i>-butyl hydroperoxide (<i>t</i>-BHP...
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MDPI AG
2022-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/24/8970 |
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| author | Woo Kwon Jung Su-Bin Park Hwa Young Yu Yong Hwan Kim Junghyun Kim |
| author_facet | Woo Kwon Jung Su-Bin Park Hwa Young Yu Yong Hwan Kim Junghyun Kim |
| author_sort | Woo Kwon Jung |
| collection | DOAJ |
| description | Esculetin is a coumarin-derived compound with antioxidant and anti-inflammatory properties. The current study aims to evaluate the therapeutic implications of esculetin on retinal dysfunction and uncover the underlying mechanisms. <i>Tert</i>-butyl hydroperoxide (<i>t</i>-BHP) at a concentration of 300 μM was used to induce oxidative stress in human retinal pigment epithelial cell line (ARPE-19) cells. Esculetin at concentrations below 250 μM did not cause cytotoxicity to ARPE-19 cells. Cell viability analysis confirmed that <i>t</i>-BHP induced oxidative injury of ARPE-19 cells. However, ARPE-19 cells were protected from <i>t</i>-BHP-induced oxidative injury by esculetin in a concentration-dependent manner. As a result of the TUNEL assay to confirm apoptosis, esculetin treatment reduced the number of TUNEL-positive cells. Esculetin down-regulated the expression levels of Bax, Caspase-3, and PARP and up-regulated the expression level of Bcl2. Collectively, this study demonstrates that esculetin exerts potent antioxidant properties in ARPE-19 cells, inhibiting <i>t</i>-BHP-induced apoptosis under the regulation of apoptotic factors. |
| first_indexed | 2024-03-09T16:02:26Z |
| format | Article |
| id | doaj.art-9675576fd25b4d4f9b36f1886002b8f2 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T16:02:26Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-9675576fd25b4d4f9b36f1886002b8f22023-11-24T17:00:33ZengMDPI AGMolecules1420-30492022-12-012724897010.3390/molecules27248970Effect of Esculetin on <i>Tert</i>-Butyl Hydroperoxide-Induced Oxidative Injury in Retinal Pigment Epithelial Cells In VitroWoo Kwon Jung0Su-Bin Park1Hwa Young Yu2Yong Hwan Kim3Junghyun Kim4Department of Oral Pathology, School of Dentistry, Jeonbuk National University, Jeonju 54896, Republic of KoreaDepartment of Oral Pathology, School of Dentistry, Jeonbuk National University, Jeonju 54896, Republic of KoreaDepartment of Oral Pathology, School of Dentistry, Jeonbuk National University, Jeonju 54896, Republic of KoreaDepartment of Oral Pathology, School of Dentistry, Jeonbuk National University, Jeonju 54896, Republic of KoreaDepartment of Oral Pathology, School of Dentistry, Jeonbuk National University, Jeonju 54896, Republic of KoreaEsculetin is a coumarin-derived compound with antioxidant and anti-inflammatory properties. The current study aims to evaluate the therapeutic implications of esculetin on retinal dysfunction and uncover the underlying mechanisms. <i>Tert</i>-butyl hydroperoxide (<i>t</i>-BHP) at a concentration of 300 μM was used to induce oxidative stress in human retinal pigment epithelial cell line (ARPE-19) cells. Esculetin at concentrations below 250 μM did not cause cytotoxicity to ARPE-19 cells. Cell viability analysis confirmed that <i>t</i>-BHP induced oxidative injury of ARPE-19 cells. However, ARPE-19 cells were protected from <i>t</i>-BHP-induced oxidative injury by esculetin in a concentration-dependent manner. As a result of the TUNEL assay to confirm apoptosis, esculetin treatment reduced the number of TUNEL-positive cells. Esculetin down-regulated the expression levels of Bax, Caspase-3, and PARP and up-regulated the expression level of Bcl2. Collectively, this study demonstrates that esculetin exerts potent antioxidant properties in ARPE-19 cells, inhibiting <i>t</i>-BHP-induced apoptosis under the regulation of apoptotic factors.https://www.mdpi.com/1420-3049/27/24/8970age-related macular degenerationapoptosisesculetinoxidative stressretinal pigment epithelial cell |
| spellingShingle | Woo Kwon Jung Su-Bin Park Hwa Young Yu Yong Hwan Kim Junghyun Kim Effect of Esculetin on <i>Tert</i>-Butyl Hydroperoxide-Induced Oxidative Injury in Retinal Pigment Epithelial Cells In Vitro Molecules age-related macular degeneration apoptosis esculetin oxidative stress retinal pigment epithelial cell |
| title | Effect of Esculetin on <i>Tert</i>-Butyl Hydroperoxide-Induced Oxidative Injury in Retinal Pigment Epithelial Cells In Vitro |
| title_full | Effect of Esculetin on <i>Tert</i>-Butyl Hydroperoxide-Induced Oxidative Injury in Retinal Pigment Epithelial Cells In Vitro |
| title_fullStr | Effect of Esculetin on <i>Tert</i>-Butyl Hydroperoxide-Induced Oxidative Injury in Retinal Pigment Epithelial Cells In Vitro |
| title_full_unstemmed | Effect of Esculetin on <i>Tert</i>-Butyl Hydroperoxide-Induced Oxidative Injury in Retinal Pigment Epithelial Cells In Vitro |
| title_short | Effect of Esculetin on <i>Tert</i>-Butyl Hydroperoxide-Induced Oxidative Injury in Retinal Pigment Epithelial Cells In Vitro |
| title_sort | effect of esculetin on i tert i butyl hydroperoxide induced oxidative injury in retinal pigment epithelial cells in vitro |
| topic | age-related macular degeneration apoptosis esculetin oxidative stress retinal pigment epithelial cell |
| url | https://www.mdpi.com/1420-3049/27/24/8970 |
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