Thermophilic Chloroflexi Dominate in the Microbial Community Associated with Coal-Fire Gas Vents in the Kuznetsk Coal Basin, Russia
Thermal ecosystems associated with areas of underground burning coal seams are rare and poorly understood in comparison with geothermal objects. We studied the microbial communities associated with gas vents from the coal-fire in the mining wastes in the Kemerovo region of the Russian Federation. Th...
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MDPI AG
2021-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/5/948 |
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| author | Vitaly V. Kadnikov Andrey V. Mardanov Alexey V. Beletsky Mikhail A. Grigoriev Olga V. Karnachuk Nikolai V. Ravin |
| author_facet | Vitaly V. Kadnikov Andrey V. Mardanov Alexey V. Beletsky Mikhail A. Grigoriev Olga V. Karnachuk Nikolai V. Ravin |
| author_sort | Vitaly V. Kadnikov |
| collection | DOAJ |
| description | Thermal ecosystems associated with areas of underground burning coal seams are rare and poorly understood in comparison with geothermal objects. We studied the microbial communities associated with gas vents from the coal-fire in the mining wastes in the Kemerovo region of the Russian Federation. The temperature of the ground heated by the hot coal gases and steam coming out to the surface was 58 °C. Analysis of the composition of microbial communities revealed the dominance of <i>Ktedonobacteria</i> (the phylum Chloroflexi), known to be capable of oxidizing hydrogen and carbon monoxide. Thermophilic hydrogenotrophic Firmicutes constituted a minor part of the community. Among the well-known thermophiles, members of the phyla Aquificae, Deinococcus-Thermus and Bacteroidetes were also found. In the upper ground layer, Acidobacteria, Verrucomicrobia, Actinobacteria, Planctomycetes, as well as Proteobacteria of the alpha and gamma classes, typical of soils, were detected; their relative abundancies decreased with depth. The phylum Verrucomicrobia was dominated by <i>Candidatus</i> Udaeobacter, aerobic heterotrophs capable of generating energy through the oxidation of hydrogen present in the atmosphere in trace amounts. Archaea made up a small part of the communities and were represented by thermophilic ammonium-oxidizers. Overall, the community was dominated by bacteria, whose cultivated relatives are able to obtain energy through the oxidation of the main components of coal gases, hydrogen and carbon monoxide, under aerobic conditions. |
| first_indexed | 2024-03-10T11:53:14Z |
| format | Article |
| id | doaj.art-9676282a0ee2403f88778208a8ca91ed |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T11:53:14Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-9676282a0ee2403f88778208a8ca91ed2023-11-21T17:30:19ZengMDPI AGMicroorganisms2076-26072021-04-019594810.3390/microorganisms9050948Thermophilic Chloroflexi Dominate in the Microbial Community Associated with Coal-Fire Gas Vents in the Kuznetsk Coal Basin, RussiaVitaly V. Kadnikov0Andrey V. Mardanov1Alexey V. Beletsky2Mikhail A. Grigoriev3Olga V. Karnachuk4Nikolai V. Ravin5Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, RussiaLaboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050 Tomsk, RussiaLaboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050 Tomsk, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, RussiaThermal ecosystems associated with areas of underground burning coal seams are rare and poorly understood in comparison with geothermal objects. We studied the microbial communities associated with gas vents from the coal-fire in the mining wastes in the Kemerovo region of the Russian Federation. The temperature of the ground heated by the hot coal gases and steam coming out to the surface was 58 °C. Analysis of the composition of microbial communities revealed the dominance of <i>Ktedonobacteria</i> (the phylum Chloroflexi), known to be capable of oxidizing hydrogen and carbon monoxide. Thermophilic hydrogenotrophic Firmicutes constituted a minor part of the community. Among the well-known thermophiles, members of the phyla Aquificae, Deinococcus-Thermus and Bacteroidetes were also found. In the upper ground layer, Acidobacteria, Verrucomicrobia, Actinobacteria, Planctomycetes, as well as Proteobacteria of the alpha and gamma classes, typical of soils, were detected; their relative abundancies decreased with depth. The phylum Verrucomicrobia was dominated by <i>Candidatus</i> Udaeobacter, aerobic heterotrophs capable of generating energy through the oxidation of hydrogen present in the atmosphere in trace amounts. Archaea made up a small part of the communities and were represented by thermophilic ammonium-oxidizers. Overall, the community was dominated by bacteria, whose cultivated relatives are able to obtain energy through the oxidation of the main components of coal gases, hydrogen and carbon monoxide, under aerobic conditions.https://www.mdpi.com/2076-2607/9/5/948thermophilescoal gaseshydrogenotrophsmicrobial communityChloroflexi<i>Candidatus</i> Udaeobacter |
| spellingShingle | Vitaly V. Kadnikov Andrey V. Mardanov Alexey V. Beletsky Mikhail A. Grigoriev Olga V. Karnachuk Nikolai V. Ravin Thermophilic Chloroflexi Dominate in the Microbial Community Associated with Coal-Fire Gas Vents in the Kuznetsk Coal Basin, Russia Microorganisms thermophiles coal gases hydrogenotrophs microbial community Chloroflexi <i>Candidatus</i> Udaeobacter |
| title | Thermophilic Chloroflexi Dominate in the Microbial Community Associated with Coal-Fire Gas Vents in the Kuznetsk Coal Basin, Russia |
| title_full | Thermophilic Chloroflexi Dominate in the Microbial Community Associated with Coal-Fire Gas Vents in the Kuznetsk Coal Basin, Russia |
| title_fullStr | Thermophilic Chloroflexi Dominate in the Microbial Community Associated with Coal-Fire Gas Vents in the Kuznetsk Coal Basin, Russia |
| title_full_unstemmed | Thermophilic Chloroflexi Dominate in the Microbial Community Associated with Coal-Fire Gas Vents in the Kuznetsk Coal Basin, Russia |
| title_short | Thermophilic Chloroflexi Dominate in the Microbial Community Associated with Coal-Fire Gas Vents in the Kuznetsk Coal Basin, Russia |
| title_sort | thermophilic chloroflexi dominate in the microbial community associated with coal fire gas vents in the kuznetsk coal basin russia |
| topic | thermophiles coal gases hydrogenotrophs microbial community Chloroflexi <i>Candidatus</i> Udaeobacter |
| url | https://www.mdpi.com/2076-2607/9/5/948 |
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