Host Specificity of Endophytic Fungi from Stem Tissue of Nature Farming Tomato (<i>Solanum lycopersicum</i> Mill.) in Japan
To understand the distribution of the cultivable fungal community in plant tissues from nature farming tomato plants, we sampled plants of seven different tomato cultivars and recovered 1742 fungal isolates from 1895 stem tissues sampled from three sites in Japan. Overall, the isolation frequency wa...
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MDPI AG
2020-07-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/10/7/1019 |
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| author | Khondoker Mohammad Golam Dastogeer Yutaro Oshita Michiko Yasuda Makoto Kanasugi Eri Matsuura Qicong Xu Shin Okazaki |
| author_facet | Khondoker Mohammad Golam Dastogeer Yutaro Oshita Michiko Yasuda Makoto Kanasugi Eri Matsuura Qicong Xu Shin Okazaki |
| author_sort | Khondoker Mohammad Golam Dastogeer |
| collection | DOAJ |
| description | To understand the distribution of the cultivable fungal community in plant tissues from nature farming tomato plants, we sampled plants of seven different tomato cultivars and recovered 1742 fungal isolates from 1895 stem tissues sampled from three sites in Japan. Overall, the isolation frequency was low (3–13%) and the isolation and colonization frequencies did not vary significantly as a function of the cultivar. The fungi were divided into 29 unique operational taxonomic units (OTUs) with 97% ITS gene sequence identity, the majority of which belong to Ascomycota (99.3%). The dominant genera of cultivable endophytic fungi were <i>Fusarium</i> (45.1%), <i>Alternaria</i> (12.8%), <i>Gibberella</i> (12.0%), and <i>Dipodascus</i> (6.8%). The alpha diversity of the fungal endophytes varied among tomato cultivars. Ordination analysis performed to investigate patterns of endophyte community assemblages on the various cultivars revealed that host cultivars had a significant impact on the endophyte community assemblages in all the study sites. Some of the taxa <i>Fusarium, Alternaria,</i> and <i>Penicillium</i> were found on all cultivars, while few were uniquely present in different cultivars. The dominant taxa may be adapted to the particular microecological and physiological conditions present in tomato stems. |
| first_indexed | 2024-03-10T18:28:50Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T18:28:50Z |
| publishDate | 2020-07-01 |
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| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-9677b29345674f5cb5f154aeb0af7bc72023-11-20T06:49:55ZengMDPI AGAgronomy2073-43952020-07-01107101910.3390/agronomy10071019Host Specificity of Endophytic Fungi from Stem Tissue of Nature Farming Tomato (<i>Solanum lycopersicum</i> Mill.) in JapanKhondoker Mohammad Golam Dastogeer0Yutaro Oshita1Michiko Yasuda2Makoto Kanasugi3Eri Matsuura4Qicong Xu5Shin Okazaki6Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, JapanFaculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, JapanGraduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, JapanGraduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Tokyo 183-8509, JapanCollege of Agriculture, Ibaraki University, Ibaraki 310-8512, JapanDepartment of Research, International Nature Farming Research Center, Nagano-ken 390-1401, JapanGraduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, JapanTo understand the distribution of the cultivable fungal community in plant tissues from nature farming tomato plants, we sampled plants of seven different tomato cultivars and recovered 1742 fungal isolates from 1895 stem tissues sampled from three sites in Japan. Overall, the isolation frequency was low (3–13%) and the isolation and colonization frequencies did not vary significantly as a function of the cultivar. The fungi were divided into 29 unique operational taxonomic units (OTUs) with 97% ITS gene sequence identity, the majority of which belong to Ascomycota (99.3%). The dominant genera of cultivable endophytic fungi were <i>Fusarium</i> (45.1%), <i>Alternaria</i> (12.8%), <i>Gibberella</i> (12.0%), and <i>Dipodascus</i> (6.8%). The alpha diversity of the fungal endophytes varied among tomato cultivars. Ordination analysis performed to investigate patterns of endophyte community assemblages on the various cultivars revealed that host cultivars had a significant impact on the endophyte community assemblages in all the study sites. Some of the taxa <i>Fusarium, Alternaria,</i> and <i>Penicillium</i> were found on all cultivars, while few were uniquely present in different cultivars. The dominant taxa may be adapted to the particular microecological and physiological conditions present in tomato stems.https://www.mdpi.com/2073-4395/10/7/1019colonization frequencydiversityfungal endophytenature farming |
| spellingShingle | Khondoker Mohammad Golam Dastogeer Yutaro Oshita Michiko Yasuda Makoto Kanasugi Eri Matsuura Qicong Xu Shin Okazaki Host Specificity of Endophytic Fungi from Stem Tissue of Nature Farming Tomato (<i>Solanum lycopersicum</i> Mill.) in Japan Agronomy colonization frequency diversity fungal endophyte nature farming |
| title | Host Specificity of Endophytic Fungi from Stem Tissue of Nature Farming Tomato (<i>Solanum lycopersicum</i> Mill.) in Japan |
| title_full | Host Specificity of Endophytic Fungi from Stem Tissue of Nature Farming Tomato (<i>Solanum lycopersicum</i> Mill.) in Japan |
| title_fullStr | Host Specificity of Endophytic Fungi from Stem Tissue of Nature Farming Tomato (<i>Solanum lycopersicum</i> Mill.) in Japan |
| title_full_unstemmed | Host Specificity of Endophytic Fungi from Stem Tissue of Nature Farming Tomato (<i>Solanum lycopersicum</i> Mill.) in Japan |
| title_short | Host Specificity of Endophytic Fungi from Stem Tissue of Nature Farming Tomato (<i>Solanum lycopersicum</i> Mill.) in Japan |
| title_sort | host specificity of endophytic fungi from stem tissue of nature farming tomato i solanum lycopersicum i mill in japan |
| topic | colonization frequency diversity fungal endophyte nature farming |
| url | https://www.mdpi.com/2073-4395/10/7/1019 |
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