Research Note: Development of rapid isothermal amplification assay for detection of duck circovirus
ABSTRACT: Duck circovirus disease (DuCVD) caused by duck circovirus (DuCV) continues to spread in recent years, which brings serious harm to the poultry industry, so early diagnosis of DuCVD is of great significance for the prevention and control of this disease. Specific primers and probes for DuCV...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579121003734 |
| _version_ | 1818730205658742784 |
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| author | Xinyue Li Chunguang Wang Wenjing Wang Zichuang Zhang Zongshu Zhang Chao Wang Tie Zhang |
| author_facet | Xinyue Li Chunguang Wang Wenjing Wang Zichuang Zhang Zongshu Zhang Chao Wang Tie Zhang |
| author_sort | Xinyue Li |
| collection | DOAJ |
| description | ABSTRACT: Duck circovirus disease (DuCVD) caused by duck circovirus (DuCV) continues to spread in recent years, which brings serious harm to the poultry industry, so early diagnosis of DuCVD is of great significance for the prevention and control of this disease. Specific primers and probes for DuCV were designed in this study. Reverse primers and probes were modified at the 5′ ends with biotin and fluorescein, respectively, and they were combined with dipsticks labeled with biotin antibodies and fluorescein antibodies to establish a recombinase-aided amplification-lateral flow dipstick (RAA-LFD) assay for detection of duck circovirus. By using this method, the reaction products reached detectable levels in about 20 min as a result of rapid amplification at a constant temperature of 37℃. The detection results could be observed by dripping the reaction products onto the dipstick within 2 to 3 min. The RAA-LFD method has good specificity and high sensitivity (102 copies/μL). Compared with conventional polymerase chain reaction (PCR), RAA-LFD has no power limit on the testing instrument, and is easy to use, saving more time and manpower, so it is more suitable for clinical detection. |
| first_indexed | 2024-12-17T22:58:05Z |
| format | Article |
| id | doaj.art-9678628bdd2f4c89b3ac564880a73ed3 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-17T22:58:05Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-9678628bdd2f4c89b3ac564880a73ed32022-12-21T21:29:29ZengElsevierPoultry Science0032-57912021-09-011009101339Research Note: Development of rapid isothermal amplification assay for detection of duck circovirusXinyue Li0Chunguang Wang1Wenjing Wang2Zichuang Zhang3Zongshu Zhang4Chao Wang5Tie Zhang6College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, ChinaCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, ChinaCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China; Corresponding author:ABSTRACT: Duck circovirus disease (DuCVD) caused by duck circovirus (DuCV) continues to spread in recent years, which brings serious harm to the poultry industry, so early diagnosis of DuCVD is of great significance for the prevention and control of this disease. Specific primers and probes for DuCV were designed in this study. Reverse primers and probes were modified at the 5′ ends with biotin and fluorescein, respectively, and they were combined with dipsticks labeled with biotin antibodies and fluorescein antibodies to establish a recombinase-aided amplification-lateral flow dipstick (RAA-LFD) assay for detection of duck circovirus. By using this method, the reaction products reached detectable levels in about 20 min as a result of rapid amplification at a constant temperature of 37℃. The detection results could be observed by dripping the reaction products onto the dipstick within 2 to 3 min. The RAA-LFD method has good specificity and high sensitivity (102 copies/μL). Compared with conventional polymerase chain reaction (PCR), RAA-LFD has no power limit on the testing instrument, and is easy to use, saving more time and manpower, so it is more suitable for clinical detection.http://www.sciencedirect.com/science/article/pii/S0032579121003734duck circovirusrecombinase-aided amplificationlateral flow dipstick |
| spellingShingle | Xinyue Li Chunguang Wang Wenjing Wang Zichuang Zhang Zongshu Zhang Chao Wang Tie Zhang Research Note: Development of rapid isothermal amplification assay for detection of duck circovirus Poultry Science duck circovirus recombinase-aided amplification lateral flow dipstick |
| title | Research Note: Development of rapid isothermal amplification assay for detection of duck circovirus |
| title_full | Research Note: Development of rapid isothermal amplification assay for detection of duck circovirus |
| title_fullStr | Research Note: Development of rapid isothermal amplification assay for detection of duck circovirus |
| title_full_unstemmed | Research Note: Development of rapid isothermal amplification assay for detection of duck circovirus |
| title_short | Research Note: Development of rapid isothermal amplification assay for detection of duck circovirus |
| title_sort | research note development of rapid isothermal amplification assay for detection of duck circovirus |
| topic | duck circovirus recombinase-aided amplification lateral flow dipstick |
| url | http://www.sciencedirect.com/science/article/pii/S0032579121003734 |
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