Isolation of a <i>Lactobacillus paracasei</i> Strain with Probiotic Attributes from Kefir Grains
Κefir is a rich source of potentially probiotic bacteria. In the present study, firstly, in vitro screening for probiotic characteristics of ten lactic acid bacteria (LAB) isolated from kefir grains was performed. Strain AGR 4 was selected for further studies. Molecular characterization of strain AG...
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MDPI AG
2020-12-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/8/12/594 |
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| author | Stavros Plessas Despoina Eugenia Kiousi Marina Rathosi Athanasios Alexopoulos Yiannis Kourkoutas Ioanna Mantzourani Alex Galanis Eugenia Bezirtzoglou |
| author_facet | Stavros Plessas Despoina Eugenia Kiousi Marina Rathosi Athanasios Alexopoulos Yiannis Kourkoutas Ioanna Mantzourani Alex Galanis Eugenia Bezirtzoglou |
| author_sort | Stavros Plessas |
| collection | DOAJ |
| description | Κefir is a rich source of potentially probiotic bacteria. In the present study, firstly, in vitro screening for probiotic characteristics of ten lactic acid bacteria (LAB) isolated from kefir grains was performed. Strain AGR 4 was selected for further studies. Molecular characterization of strain AGR 4, confirmed that AGR 4 belongs to the <i>Lactobacillus paracasei</i> (reclassified to <i>Lacticaseibacillus paracasei</i> subsp. <i>paracasei</i>) species. Further testing revealed that <i>L. paracasei</i> AGR 4 displayed adhesion capacity on human adenocarcinoma cells, HT-29, similar to that of the reference strain, <i>L. casei</i> ATCC 393. In addition, the novel strain exerted significant time- and dose-dependent antiproliferative activity against HT-29 cells and human melanoma cell line, A375, as demonstrated by the sulforhodamine B cytotoxicity assay. Flow cytometry analysis was employed to investigate the mechanism of cellular death; however, it was found that AGR 4 did not act by inducing cell cycle arrest and/or apoptotic cell death. Taken together, these findings promote the probiotic character of the newly isolated strain <i>L. paracasei</i> AGR 4, while further studies are needed for the detailed description of its biological properties. |
| first_indexed | 2024-03-10T14:09:53Z |
| format | Article |
| id | doaj.art-96789f663cf04af0a7ff0960ed6a30c2 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T14:09:53Z |
| publishDate | 2020-12-01 |
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| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-96789f663cf04af0a7ff0960ed6a30c22023-11-21T00:19:20ZengMDPI AGBiomedicines2227-90592020-12-0181259410.3390/biomedicines8120594Isolation of a <i>Lactobacillus paracasei</i> Strain with Probiotic Attributes from Kefir GrainsStavros Plessas0Despoina Eugenia Kiousi1Marina Rathosi2Athanasios Alexopoulos3Yiannis Kourkoutas4Ioanna Mantzourani5Alex Galanis6Eugenia Bezirtzoglou7Laboratory of Food Processing, Faculty of Agricultural Development, Democritus University of Thrace, 68200 Orestiada, GreeceDepartment of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, GreeceDepartment of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, GreeceLaboratory of Food Processing, Faculty of Agricultural Development, Democritus University of Thrace, 68200 Orestiada, GreeceLaboratory of Applied Microbiology and Biotechnology, Department of Molecular Biology and Genetics, Democritus University of Thrace, 68100 Alexandroupolis, GreeceLaboratory of Food Processing, Faculty of Agricultural Development, Democritus University of Thrace, 68200 Orestiada, GreeceDepartment of Molecular Biology and Genetics, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, GreeceLaboratory of Hygiene and Environmental Protection, Medical School, Faculty of Health Sciences, Democritus University of Thrace, 68100 Alexandroupolis, GreeceΚefir is a rich source of potentially probiotic bacteria. In the present study, firstly, in vitro screening for probiotic characteristics of ten lactic acid bacteria (LAB) isolated from kefir grains was performed. Strain AGR 4 was selected for further studies. Molecular characterization of strain AGR 4, confirmed that AGR 4 belongs to the <i>Lactobacillus paracasei</i> (reclassified to <i>Lacticaseibacillus paracasei</i> subsp. <i>paracasei</i>) species. Further testing revealed that <i>L. paracasei</i> AGR 4 displayed adhesion capacity on human adenocarcinoma cells, HT-29, similar to that of the reference strain, <i>L. casei</i> ATCC 393. In addition, the novel strain exerted significant time- and dose-dependent antiproliferative activity against HT-29 cells and human melanoma cell line, A375, as demonstrated by the sulforhodamine B cytotoxicity assay. Flow cytometry analysis was employed to investigate the mechanism of cellular death; however, it was found that AGR 4 did not act by inducing cell cycle arrest and/or apoptotic cell death. Taken together, these findings promote the probiotic character of the newly isolated strain <i>L. paracasei</i> AGR 4, while further studies are needed for the detailed description of its biological properties.https://www.mdpi.com/2227-9059/8/12/594kefirlactic acid bacteriaprobioticsadherencecancer cellsapoptosis |
| spellingShingle | Stavros Plessas Despoina Eugenia Kiousi Marina Rathosi Athanasios Alexopoulos Yiannis Kourkoutas Ioanna Mantzourani Alex Galanis Eugenia Bezirtzoglou Isolation of a <i>Lactobacillus paracasei</i> Strain with Probiotic Attributes from Kefir Grains Biomedicines kefir lactic acid bacteria probiotics adherence cancer cells apoptosis |
| title | Isolation of a <i>Lactobacillus paracasei</i> Strain with Probiotic Attributes from Kefir Grains |
| title_full | Isolation of a <i>Lactobacillus paracasei</i> Strain with Probiotic Attributes from Kefir Grains |
| title_fullStr | Isolation of a <i>Lactobacillus paracasei</i> Strain with Probiotic Attributes from Kefir Grains |
| title_full_unstemmed | Isolation of a <i>Lactobacillus paracasei</i> Strain with Probiotic Attributes from Kefir Grains |
| title_short | Isolation of a <i>Lactobacillus paracasei</i> Strain with Probiotic Attributes from Kefir Grains |
| title_sort | isolation of a i lactobacillus paracasei i strain with probiotic attributes from kefir grains |
| topic | kefir lactic acid bacteria probiotics adherence cancer cells apoptosis |
| url | https://www.mdpi.com/2227-9059/8/12/594 |
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