| Summary: | An <i>Aspergillus fumigatus</i> KMM 4631 strain was previously isolated from a Pacific soft coral <i>Sinularia</i> sp. sample and was found to be a source of a number of bioactive secondary metabolites. The aims of this work are the confirmation of this strain’ identification based on ITS, <i>BenA</i>, <i>CaM</i>, and <i>RPB2</i> regions/gene sequences and the investigation of secondary metabolite profiles of <i>Aspergillus fumigatus</i> KMM 4631 culture and its co-cultures with <i>Penicillium hispanicum</i> KMM 4689, <i>Amphichorda</i> sp. KMM 4639, <i>Penicillium</i> sp. KMM 4672, and <i>Asteromyces cruciatus</i> KMM 4696 from the Collection of Marine Microorganisms (PIBOC FEB RAS, Vladivostok, Russia). Moreover, the DPPH-radical scavenging activity, urease inhibition, and cytotoxicity of joint fungal cultures’ extracts on HepG2 cells were tested. The detailed UPLC MS qTOF investigation resulted in the identification and annotation of indolediketopiperazine, quinazoline, and tryptoquivaline-related alkaloids as well as a number of polyketides (totally 20 compounds) in the extract of <i>Aspergillus fumigatus</i> KMM 4631. The metabolite profiles of the co-cultures of <i>A. fumigatus</i> with <i>Penicillium hispanicum</i>, <i>Penicillium</i> sp., and <i>Amphichorda</i> sp. were similar to those of <i>Penicillium hispanicum</i>, <i>Penicillium</i> sp., and <i>Amphichorda</i> sp. monocultures. The metabolite profile of the co-culture of <i>A. fumigatus</i> with <i>Asteromyces cruciatus</i> differed from that of each monoculture and may be more promising for the isolation of new compounds.
|
|---|