Development of a New Method Based on Copper Sulfide Nanoparticles for the Determination of Fentanyl in Biological Samples
Background: Fentanyl is an opioid analgesic drug extensively used to alleviate pain with no consciousness loss. To address the fentanyl crisis from the criminal and medical perspectives,the development of a free drug determination methodology for this drug is crucial. Methods: A fluorescent nanoprob...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2022-12-01
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| Series: | ImmunoAnalysis |
| Subjects: | |
| Online Access: | https://ia.tbzmed.ac.ir/PDF/ia-2-5.pdf |
| _version_ | 1797817509462671360 |
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| author | Sedigheh Mohammadzadeh Zahra Karimzadeh |
| author_facet | Sedigheh Mohammadzadeh Zahra Karimzadeh |
| author_sort | Sedigheh Mohammadzadeh |
| collection | DOAJ |
| description | Background: Fentanyl is an opioid analgesic drug extensively used to alleviate pain with no consciousness loss. To address the fentanyl crisis from the criminal and medical perspectives,the development of a free drug determination methodology for this drug is crucial. Methods: A fluorescent nanoprobe based on copper sulfide nanoparticles (CuS NPs) is developed for the determination of fentanyl in exhaled breath condensate (EBC). NPs are synthesized according to a hydrothermal method and their size and morphology are characterized via X-ray diffraction (XRD), Fourier-transformed infrared (FTIR), and TEM. The fluorescence intensity of the nanoprobe is enhanced in the presence of fentanyl. The affinity of CuS NPs to complex formation with fentanyl results in blocking non-radiative e−/h+recombination defect sites on the surface of NPs and consequently enhancing the signal intensity. One at a time optimization method was used for the optimization of reaction conditions. Results: Under the optimized conditions, a low limit of detection (LOD) of 0.008 µg mL-1 was obtained for fentanyl determination. Furthermore, a linear relationship is found between the analytical response and the concentration of fentanyl in the range of 0.01-2.0 µg mL-1 with a relative standard deviation of<2.5%. Conclusion: The validated method is applied for the determination of fentanyl in the EBC of patients receiving fentanyl treatment. |
| first_indexed | 2024-03-13T08:54:30Z |
| format | Article |
| id | doaj.art-967af4db215c43e185c972549fcb4d99 |
| institution | Directory Open Access Journal |
| issn | 2783-2589 |
| language | English |
| last_indexed | 2024-03-13T08:54:30Z |
| publishDate | 2022-12-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | ImmunoAnalysis |
| spelling | doaj.art-967af4db215c43e185c972549fcb4d992023-05-29T05:15:31ZengTabriz University of Medical SciencesImmunoAnalysis2783-25892022-12-01215510.34172/ia.2022.05ia-3031Development of a New Method Based on Copper Sulfide Nanoparticles for the Determination of Fentanyl in Biological SamplesSedigheh Mohammadzadeh0Zahra Karimzadeh1Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranPharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranBackground: Fentanyl is an opioid analgesic drug extensively used to alleviate pain with no consciousness loss. To address the fentanyl crisis from the criminal and medical perspectives,the development of a free drug determination methodology for this drug is crucial. Methods: A fluorescent nanoprobe based on copper sulfide nanoparticles (CuS NPs) is developed for the determination of fentanyl in exhaled breath condensate (EBC). NPs are synthesized according to a hydrothermal method and their size and morphology are characterized via X-ray diffraction (XRD), Fourier-transformed infrared (FTIR), and TEM. The fluorescence intensity of the nanoprobe is enhanced in the presence of fentanyl. The affinity of CuS NPs to complex formation with fentanyl results in blocking non-radiative e−/h+recombination defect sites on the surface of NPs and consequently enhancing the signal intensity. One at a time optimization method was used for the optimization of reaction conditions. Results: Under the optimized conditions, a low limit of detection (LOD) of 0.008 µg mL-1 was obtained for fentanyl determination. Furthermore, a linear relationship is found between the analytical response and the concentration of fentanyl in the range of 0.01-2.0 µg mL-1 with a relative standard deviation of<2.5%. Conclusion: The validated method is applied for the determination of fentanyl in the EBC of patients receiving fentanyl treatment.https://ia.tbzmed.ac.ir/PDF/ia-2-5.pdffentanylcopper sulfide nanoparticlesspectrofluorimetryexhaled breath condensate |
| spellingShingle | Sedigheh Mohammadzadeh Zahra Karimzadeh Development of a New Method Based on Copper Sulfide Nanoparticles for the Determination of Fentanyl in Biological Samples ImmunoAnalysis fentanyl copper sulfide nanoparticles spectrofluorimetry exhaled breath condensate |
| title | Development of a New Method Based on Copper Sulfide Nanoparticles for the Determination of Fentanyl in Biological Samples |
| title_full | Development of a New Method Based on Copper Sulfide Nanoparticles for the Determination of Fentanyl in Biological Samples |
| title_fullStr | Development of a New Method Based on Copper Sulfide Nanoparticles for the Determination of Fentanyl in Biological Samples |
| title_full_unstemmed | Development of a New Method Based on Copper Sulfide Nanoparticles for the Determination of Fentanyl in Biological Samples |
| title_short | Development of a New Method Based on Copper Sulfide Nanoparticles for the Determination of Fentanyl in Biological Samples |
| title_sort | development of a new method based on copper sulfide nanoparticles for the determination of fentanyl in biological samples |
| topic | fentanyl copper sulfide nanoparticles spectrofluorimetry exhaled breath condensate |
| url | https://ia.tbzmed.ac.ir/PDF/ia-2-5.pdf |
| work_keys_str_mv | AT sedighehmohammadzadeh developmentofanewmethodbasedoncoppersulfidenanoparticlesforthedeterminationoffentanylinbiologicalsamples AT zahrakarimzadeh developmentofanewmethodbasedoncoppersulfidenanoparticlesforthedeterminationoffentanylinbiologicalsamples |