Ultrasonic-Assisted Aqueous Two-Phase Extraction and Properties of Water-Soluble Polysaccharides from <i>Malus hupehensis</i>
<i>Malus hupehensis</i> (<i>M. hupehensis</i>), an edible and medicinal plant with significant antioxidant and hypoglycemic activity, has been applied to new resource foods. However, the structural characterization and biological effects of its polysaccharides (MHP) are less...
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MDPI AG
2021-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/8/2213 |
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| author | Pengcheng Li Hongkun Xue Mi Xiao Jintian Tang Hansong Yu Yanqi Su Xu Cai |
| author_facet | Pengcheng Li Hongkun Xue Mi Xiao Jintian Tang Hansong Yu Yanqi Su Xu Cai |
| author_sort | Pengcheng Li |
| collection | DOAJ |
| description | <i>Malus hupehensis</i> (<i>M. hupehensis</i>), an edible and medicinal plant with significant antioxidant and hypoglycemic activity, has been applied to new resource foods. However, the structural characterization and biological effects of its polysaccharides (MHP) are less known. The optimum extraction parameters to achieve the highest extraction efficiency (47.63%), the yield (1.68%) and purity of MHP (89.6%) by ultrasonic-assisted aqueous two-phase system (ATPS) were obtained under the liquid-to-solid ratio of 23 g/mL, ultrasonic power of 65 W, and ultrasonic time of 33 min. According to the analysis results, MHP was composed of Man, GlcA, Rha, GalA, Glc, Gal, Xyl, Ara, and Fuc, in which Ara and Gal were the main components, and the content of GlcA was the lowest. In in vitro activity analysis, MHP showed a significant antioxidant capacity, and an inhibition activity of α-glucosidase and the advanced glycation end products (AGEs) formation in the BSA/Glc reaction model. MHP interacted with α-glucosidase and changed the internal microenvironment of the enzyme, and inhibited the AGEs formation, which provides more evidence for the antihyperglycemic mechanism of MHP. The results suggest that ATPS is an efficient and environmentally friendly solvent system, and <i>M. hupehensis</i> has broad application prospects in functional foods, healthcare products, and pharmaceuticals. |
| first_indexed | 2024-03-10T12:24:15Z |
| format | Article |
| id | doaj.art-967bf576ea404347bd7408bfe834725a |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T12:24:15Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-967bf576ea404347bd7408bfe834725a2023-11-21T15:13:09ZengMDPI AGMolecules1420-30492021-04-01268221310.3390/molecules26082213Ultrasonic-Assisted Aqueous Two-Phase Extraction and Properties of Water-Soluble Polysaccharides from <i>Malus hupehensis</i>Pengcheng Li0Hongkun Xue1Mi Xiao2Jintian Tang3Hansong Yu4Yanqi Su5Xu Cai6China Pharmaceutical Preparation Section, Huazhong University of Science and Technology Union Jiangbei Hospital/Wuhan Caidian People’s Hospital, Wuhan 430100, ChinaKey Laboratory of Particle & Radiation Imaging, Ministry of Education, Department of Engineering Physics, Tsinghua University, Beijing 100084, ChinaChina Pharmaceutical Preparation Section, Huazhong University of Science and Technology Union Jiangbei Hospital/Wuhan Caidian People’s Hospital, Wuhan 430100, ChinaKey Laboratory of Particle & Radiation Imaging, Ministry of Education, Department of Engineering Physics, Tsinghua University, Beijing 100084, ChinaCollege of Food Science and Engineering, Jilin Agricultural University, Changchun 130033, ChinaChina Pharmaceutical Preparation Section, Huazhong University of Science and Technology Union Jiangbei Hospital/Wuhan Caidian People’s Hospital, Wuhan 430100, ChinaKey Laboratory of Particle & Radiation Imaging, Ministry of Education, Department of Engineering Physics, Tsinghua University, Beijing 100084, China<i>Malus hupehensis</i> (<i>M. hupehensis</i>), an edible and medicinal plant with significant antioxidant and hypoglycemic activity, has been applied to new resource foods. However, the structural characterization and biological effects of its polysaccharides (MHP) are less known. The optimum extraction parameters to achieve the highest extraction efficiency (47.63%), the yield (1.68%) and purity of MHP (89.6%) by ultrasonic-assisted aqueous two-phase system (ATPS) were obtained under the liquid-to-solid ratio of 23 g/mL, ultrasonic power of 65 W, and ultrasonic time of 33 min. According to the analysis results, MHP was composed of Man, GlcA, Rha, GalA, Glc, Gal, Xyl, Ara, and Fuc, in which Ara and Gal were the main components, and the content of GlcA was the lowest. In in vitro activity analysis, MHP showed a significant antioxidant capacity, and an inhibition activity of α-glucosidase and the advanced glycation end products (AGEs) formation in the BSA/Glc reaction model. MHP interacted with α-glucosidase and changed the internal microenvironment of the enzyme, and inhibited the AGEs formation, which provides more evidence for the antihyperglycemic mechanism of MHP. The results suggest that ATPS is an efficient and environmentally friendly solvent system, and <i>M. hupehensis</i> has broad application prospects in functional foods, healthcare products, and pharmaceuticals.https://www.mdpi.com/1420-3049/26/8/2213<i>Malus hupehensis</i>polysaccharideultrasonic assisted aqueous two-phase systemstructural characterization |
| spellingShingle | Pengcheng Li Hongkun Xue Mi Xiao Jintian Tang Hansong Yu Yanqi Su Xu Cai Ultrasonic-Assisted Aqueous Two-Phase Extraction and Properties of Water-Soluble Polysaccharides from <i>Malus hupehensis</i> Molecules <i>Malus hupehensis</i> polysaccharide ultrasonic assisted aqueous two-phase system structural characterization |
| title | Ultrasonic-Assisted Aqueous Two-Phase Extraction and Properties of Water-Soluble Polysaccharides from <i>Malus hupehensis</i> |
| title_full | Ultrasonic-Assisted Aqueous Two-Phase Extraction and Properties of Water-Soluble Polysaccharides from <i>Malus hupehensis</i> |
| title_fullStr | Ultrasonic-Assisted Aqueous Two-Phase Extraction and Properties of Water-Soluble Polysaccharides from <i>Malus hupehensis</i> |
| title_full_unstemmed | Ultrasonic-Assisted Aqueous Two-Phase Extraction and Properties of Water-Soluble Polysaccharides from <i>Malus hupehensis</i> |
| title_short | Ultrasonic-Assisted Aqueous Two-Phase Extraction and Properties of Water-Soluble Polysaccharides from <i>Malus hupehensis</i> |
| title_sort | ultrasonic assisted aqueous two phase extraction and properties of water soluble polysaccharides from i malus hupehensis i |
| topic | <i>Malus hupehensis</i> polysaccharide ultrasonic assisted aqueous two-phase system structural characterization |
| url | https://www.mdpi.com/1420-3049/26/8/2213 |
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