Capsular polysaccharide determines the serotyping of Riemerella anatipestifer
ABSTRACT Riemerella anatipestifer (R. anatipestifer) is a Gram-negative pathogen that causes significant economic losses to the farming industry. The polysaccharides that determine serotype are well understood in other bacteria; they have not yet been fully characterized in R. anatipestifer. In this...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-12-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.01804-23 |
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| author | Yanling Liu Shuxin Luo Zhishuang Yang Mingshu Wang Renyong Jia Shun Chen Mafeng Liu Xinxin Zhao Qiao Yang Ying Wu Shaqiu Zhang Juan Huang Xumin Ou Sai Mao Qun Gao Di Sun Bin Tian Anchun Cheng Dekang Zhu |
| author_facet | Yanling Liu Shuxin Luo Zhishuang Yang Mingshu Wang Renyong Jia Shun Chen Mafeng Liu Xinxin Zhao Qiao Yang Ying Wu Shaqiu Zhang Juan Huang Xumin Ou Sai Mao Qun Gao Di Sun Bin Tian Anchun Cheng Dekang Zhu |
| author_sort | Yanling Liu |
| collection | DOAJ |
| description | ABSTRACT Riemerella anatipestifer (R. anatipestifer) is a Gram-negative pathogen that causes significant economic losses to the farming industry. The polysaccharides that determine serotype are well understood in other bacteria; they have not yet been fully characterized in R. anatipestifer. In this study, we unexpectedly discovered a strain, RCAD0392, that is capable of cross-agglutination. Whole genome sequencing revealed base mutations disrupting a gene on its capsular polysaccharide synthesis gene cluster. By constructing homologous gene deletion mutants in CH-2, we demonstrated that deletion of this gene leads to altered serological phenotypes. India ink and transmission electron microscopy experiments revealed the disappearance of capsule on the surface of the bacteria, indicating the association of the gene with capsule synthesis. In addition, agar-gel precipitin tests showed that lipopolysaccharide binds to antisera of multiple serotypes, while the capsular polysaccharide only binds to the corresponding antisera. This suggests that capsular polysaccharide is the specific antigen that determines the serotype of R. anatipestifer. IMPORTANCE Riemerella anatipestifer (R. anatipestifer) is one of the most important veterinary pathogens with at least 21 serotypes. However, the exact polysaccharide(s) that determine R. anatipestifer serotype is still unknown. This study has provided a preliminary exploration of the relationship between capsular polysaccharides and serotyping in R. anatipestifer and suggests possible directions for further investigation of the genetic basis of serotypes in this bacterium. |
| first_indexed | 2024-03-09T00:05:09Z |
| format | Article |
| id | doaj.art-967c88b9853144b7a768f568bf3c69ff |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-09T00:05:09Z |
| publishDate | 2023-12-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-967c88b9853144b7a768f568bf3c69ff2023-12-12T13:17:21ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-12-0111610.1128/spectrum.01804-23Capsular polysaccharide determines the serotyping of Riemerella anatipestiferYanling Liu0Shuxin Luo1Zhishuang Yang2Mingshu Wang3Renyong Jia4Shun Chen5Mafeng Liu6Xinxin Zhao7Qiao Yang8Ying Wu9Shaqiu Zhang10Juan Huang11Xumin Ou12Sai Mao13Qun Gao14Di Sun15Bin Tian16Anchun Cheng17Dekang Zhu18Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaResearch Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University , Chengdu, Sichuan, ChinaABSTRACT Riemerella anatipestifer (R. anatipestifer) is a Gram-negative pathogen that causes significant economic losses to the farming industry. The polysaccharides that determine serotype are well understood in other bacteria; they have not yet been fully characterized in R. anatipestifer. In this study, we unexpectedly discovered a strain, RCAD0392, that is capable of cross-agglutination. Whole genome sequencing revealed base mutations disrupting a gene on its capsular polysaccharide synthesis gene cluster. By constructing homologous gene deletion mutants in CH-2, we demonstrated that deletion of this gene leads to altered serological phenotypes. India ink and transmission electron microscopy experiments revealed the disappearance of capsule on the surface of the bacteria, indicating the association of the gene with capsule synthesis. In addition, agar-gel precipitin tests showed that lipopolysaccharide binds to antisera of multiple serotypes, while the capsular polysaccharide only binds to the corresponding antisera. This suggests that capsular polysaccharide is the specific antigen that determines the serotype of R. anatipestifer. IMPORTANCE Riemerella anatipestifer (R. anatipestifer) is one of the most important veterinary pathogens with at least 21 serotypes. However, the exact polysaccharide(s) that determine R. anatipestifer serotype is still unknown. This study has provided a preliminary exploration of the relationship between capsular polysaccharides and serotyping in R. anatipestifer and suggests possible directions for further investigation of the genetic basis of serotypes in this bacterium.https://journals.asm.org/doi/10.1128/spectrum.01804-23Riemerella anatipestifercapsuleserotypingcapsular polysaccharidelipopolysaccharide |
| spellingShingle | Yanling Liu Shuxin Luo Zhishuang Yang Mingshu Wang Renyong Jia Shun Chen Mafeng Liu Xinxin Zhao Qiao Yang Ying Wu Shaqiu Zhang Juan Huang Xumin Ou Sai Mao Qun Gao Di Sun Bin Tian Anchun Cheng Dekang Zhu Capsular polysaccharide determines the serotyping of Riemerella anatipestifer Microbiology Spectrum Riemerella anatipestifer capsule serotyping capsular polysaccharide lipopolysaccharide |
| title | Capsular polysaccharide determines the serotyping of Riemerella anatipestifer |
| title_full | Capsular polysaccharide determines the serotyping of Riemerella anatipestifer |
| title_fullStr | Capsular polysaccharide determines the serotyping of Riemerella anatipestifer |
| title_full_unstemmed | Capsular polysaccharide determines the serotyping of Riemerella anatipestifer |
| title_short | Capsular polysaccharide determines the serotyping of Riemerella anatipestifer |
| title_sort | capsular polysaccharide determines the serotyping of riemerella anatipestifer |
| topic | Riemerella anatipestifer capsule serotyping capsular polysaccharide lipopolysaccharide |
| url | https://journals.asm.org/doi/10.1128/spectrum.01804-23 |
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