Extended-spectrum beta-lactamase genes among Gram negative bacilli isolates from Egyptian children with diarrhea
Background: Faecal carriage of ESBL-producing bacteria is a potential risk for transmission and infection. Antibiotic resistance in diarrheal children is considered a risk factor for these young aged ones and limit treatment options. This study aimed to investigate the frequency of ESBL-producing...
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| Format: | Article |
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Zagazig University, Faculty of Medicine
2023-11-01
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| Series: | Microbes and Infectious Diseases |
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| Online Access: | https://mid.journals.ekb.eg/article_312429_6034287d616ebaa5d31616c8637eee6c.pdf |
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| author | ragaa Rashwan Shereen Galal Doaa Abd EL-kareem Aliaa Ghandour Rawhia Abd El-Hamid |
| author_facet | ragaa Rashwan Shereen Galal Doaa Abd EL-kareem Aliaa Ghandour Rawhia Abd El-Hamid |
| author_sort | ragaa Rashwan |
| collection | DOAJ |
| description | Background: Faecal carriage of ESBL-producing bacteria is a potential risk for transmission and infection. Antibiotic resistance in diarrheal children is considered a risk factor for these young aged ones and limit treatment options. This study aimed to investigate the frequency of ESBL-producing Gram negative bacilli (GNB) phenotypically by Vitek and genotypically by PCR. Method: Seventy-three diarrheal isolates from outpatient children at of age range from less than 6 months to 18 months were isolated. Bacterial strains were identified and subjected to antimicrobial susceptibility testing by using the automated Vitek 2 compact system. All isolates were screened for ESBL phenotypically by Vitek and genotypically by PCR for blaCTXM, blaTEM and blaSHV. Results: Seventy three different GNB identified by Vitek system. The frequency of Escherichia coli was 68.5%, Acinetobacter haemolytica was 7 /73 (9.6%), Pseudomonas species (sp.) 4/73 (5.5%), Proteus mirabilis was (2.7%), Salmonella sp. was (1.4%), Enterobacter sp. was (6.8%), Aeromonas salmonicida was (1.4%), and Klebsiella was (4.1%). ESBL carriage was significantly high among isolated strains (64.4 %). High frequency of multidrug resistance (MDR) (90.4%) was found. High resistance was detected to ampicillin (98.6%), followed by cefazolin (93.2%) and ceftazidime (82.2%), trimethoprim-sulphamethoxazol (76.7%). Resistance to carbapenems was detected as (16.4%). blaCTX-M positive was (78.1%), blaTEM positive (53.4%), blaSHV positive (31.5%). Conclusions: The current study reported a high rate of faecal carriage of ESBL-producing and MDR GNB and children below 2 years of age. Resistance was observed to the available antimicrobials that are used for children in treatment. This leaves few treatment options for infections caused by these bacteria. |
| first_indexed | 2024-03-11T11:56:00Z |
| format | Article |
| id | doaj.art-967dcde9e08b430c943efacaca620edd |
| institution | Directory Open Access Journal |
| issn | 2682-4132 2682-4140 |
| language | English |
| last_indexed | 2024-03-11T11:56:00Z |
| publishDate | 2023-11-01 |
| publisher | Zagazig University, Faculty of Medicine |
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| series | Microbes and Infectious Diseases |
| spelling | doaj.art-967dcde9e08b430c943efacaca620edd2023-11-08T19:05:38ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402023-11-01441312132410.21608/mid.2023.222631.1563312429Extended-spectrum beta-lactamase genes among Gram negative bacilli isolates from Egyptian children with diarrhearagaa Rashwan0Shereen Galal1Doaa Abd EL-kareem2Aliaa Ghandour3Rawhia Abd El-Hamid4Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, EgyptPediatrics, Gastroenterology, Hepatology, Faculty of Medicine, Assiut University Children HospitalDepartment of Clinical Pathology, Faculty of Medicine, Assiut University HospitalMedical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, EgyptMedical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, EgyptBackground: Faecal carriage of ESBL-producing bacteria is a potential risk for transmission and infection. Antibiotic resistance in diarrheal children is considered a risk factor for these young aged ones and limit treatment options. This study aimed to investigate the frequency of ESBL-producing Gram negative bacilli (GNB) phenotypically by Vitek and genotypically by PCR. Method: Seventy-three diarrheal isolates from outpatient children at of age range from less than 6 months to 18 months were isolated. Bacterial strains were identified and subjected to antimicrobial susceptibility testing by using the automated Vitek 2 compact system. All isolates were screened for ESBL phenotypically by Vitek and genotypically by PCR for blaCTXM, blaTEM and blaSHV. Results: Seventy three different GNB identified by Vitek system. The frequency of Escherichia coli was 68.5%, Acinetobacter haemolytica was 7 /73 (9.6%), Pseudomonas species (sp.) 4/73 (5.5%), Proteus mirabilis was (2.7%), Salmonella sp. was (1.4%), Enterobacter sp. was (6.8%), Aeromonas salmonicida was (1.4%), and Klebsiella was (4.1%). ESBL carriage was significantly high among isolated strains (64.4 %). High frequency of multidrug resistance (MDR) (90.4%) was found. High resistance was detected to ampicillin (98.6%), followed by cefazolin (93.2%) and ceftazidime (82.2%), trimethoprim-sulphamethoxazol (76.7%). Resistance to carbapenems was detected as (16.4%). blaCTX-M positive was (78.1%), blaTEM positive (53.4%), blaSHV positive (31.5%). Conclusions: The current study reported a high rate of faecal carriage of ESBL-producing and MDR GNB and children below 2 years of age. Resistance was observed to the available antimicrobials that are used for children in treatment. This leaves few treatment options for infections caused by these bacteria.https://mid.journals.ekb.eg/article_312429_6034287d616ebaa5d31616c8637eee6c.pdfgram negative bacilliesblblactxmblatemblashv |
| spellingShingle | ragaa Rashwan Shereen Galal Doaa Abd EL-kareem Aliaa Ghandour Rawhia Abd El-Hamid Extended-spectrum beta-lactamase genes among Gram negative bacilli isolates from Egyptian children with diarrhea Microbes and Infectious Diseases gram negative bacilli esbl blactxm blatem blashv |
| title | Extended-spectrum beta-lactamase genes among Gram negative bacilli isolates from Egyptian children with diarrhea |
| title_full | Extended-spectrum beta-lactamase genes among Gram negative bacilli isolates from Egyptian children with diarrhea |
| title_fullStr | Extended-spectrum beta-lactamase genes among Gram negative bacilli isolates from Egyptian children with diarrhea |
| title_full_unstemmed | Extended-spectrum beta-lactamase genes among Gram negative bacilli isolates from Egyptian children with diarrhea |
| title_short | Extended-spectrum beta-lactamase genes among Gram negative bacilli isolates from Egyptian children with diarrhea |
| title_sort | extended spectrum beta lactamase genes among gram negative bacilli isolates from egyptian children with diarrhea |
| topic | gram negative bacilli esbl blactxm blatem blashv |
| url | https://mid.journals.ekb.eg/article_312429_6034287d616ebaa5d31616c8637eee6c.pdf |
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