Experimental study on the optimization of ANM33 release in foam cells
Given the miR-33’s mechanistic relationships with multiple etiological factors in the pathogenesis of atherosclerosis (AS), we investigated the therapeutic potentials of dual-targeted microbubbles (HA-PANBs) in foam cell-specific release of anti-miR-33 (ANM33) oligonucleotides, resulting in the earl...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-02-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2022-0564 |
| _version_ | 1797848756470677504 |
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| author | Yuan Chen Liu Liyun Tayier Baihetiya Ma Ting Guan Lina Mu Yuming Li Yanhong |
| author_facet | Yuan Chen Liu Liyun Tayier Baihetiya Ma Ting Guan Lina Mu Yuming Li Yanhong |
| author_sort | Yuan Chen |
| collection | DOAJ |
| description | Given the miR-33’s mechanistic relationships with multiple etiological factors in the pathogenesis of atherosclerosis (AS), we investigated the therapeutic potentials of dual-targeted microbubbles (HA-PANBs) in foam cell-specific release of anti-miR-33 (ANM33) oligonucleotides, resulting in the early prevention of AS progression and severity. The intracellular localization, loading optimization, and therapeutic effects of HA-PANBs were examined in detail in a co-cultured cell model of phagocytosis. Compared with non-targeting nanobubbles (NBs) and single-targeted microbubbles as controls, HA-PANBs efficiently delivered the ANM33 specifically to foam cells via sustained release, exhibiting its clinical value in mediating RNA silencing. Moreover, when used at a dose of 12 µg/mL HA-PANBs per 107 cells for 48 h, a higher release rate and drug efficacy were observed. Therefore, HA-PANBs, effectively targeting early AS foam cells, may represent a novel and optimal gene therapy approach for AS management. |
| first_indexed | 2024-04-09T18:33:11Z |
| format | Article |
| id | doaj.art-967e1417fe864c4fa5e94d165ada7491 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-04-09T18:33:11Z |
| publishDate | 2023-02-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-967e1417fe864c4fa5e94d165ada74912023-04-11T17:07:13ZengDe GruyterOpen Life Sciences2391-54122023-02-011819203310.1515/biol-2022-0564Experimental study on the optimization of ANM33 release in foam cellsYuan Chen0Liu Liyun1Tayier Baihetiya2Ma Ting3Guan Lina4Mu Yuming5Li Yanhong6Department of Echocardiography, First Affiliated Hospital of Xinjiang Medical University, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Urumqi, ChinaDepartment of Echocardiography, First Affiliated Hospital of Xinjiang Medical University, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Urumqi, ChinaDepartment of Echocardiography, First Affiliated Hospital of Xinjiang Medical University, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Urumqi, ChinaDepartment of Echocardiography, First Affiliated Hospital of Xinjiang Medical University, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Urumqi, ChinaDepartment of Echocardiography, First Affiliated Hospital of Xinjiang Medical University, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Urumqi, ChinaDepartment of Echocardiography, First Affiliated Hospital of Xinjiang Medical University, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Urumqi, ChinaDepartment of Echocardiography, First Affiliated Hospital of Xinjiang Medical University, State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Urumqi, ChinaGiven the miR-33’s mechanistic relationships with multiple etiological factors in the pathogenesis of atherosclerosis (AS), we investigated the therapeutic potentials of dual-targeted microbubbles (HA-PANBs) in foam cell-specific release of anti-miR-33 (ANM33) oligonucleotides, resulting in the early prevention of AS progression and severity. The intracellular localization, loading optimization, and therapeutic effects of HA-PANBs were examined in detail in a co-cultured cell model of phagocytosis. Compared with non-targeting nanobubbles (NBs) and single-targeted microbubbles as controls, HA-PANBs efficiently delivered the ANM33 specifically to foam cells via sustained release, exhibiting its clinical value in mediating RNA silencing. Moreover, when used at a dose of 12 µg/mL HA-PANBs per 107 cells for 48 h, a higher release rate and drug efficacy were observed. Therefore, HA-PANBs, effectively targeting early AS foam cells, may represent a novel and optimal gene therapy approach for AS management.https://doi.org/10.1515/biol-2022-0564drug deliverydual targetingfoam cellsatherosclerosis |
| spellingShingle | Yuan Chen Liu Liyun Tayier Baihetiya Ma Ting Guan Lina Mu Yuming Li Yanhong Experimental study on the optimization of ANM33 release in foam cells Open Life Sciences drug delivery dual targeting foam cells atherosclerosis |
| title | Experimental study on the optimization of ANM33 release in foam cells |
| title_full | Experimental study on the optimization of ANM33 release in foam cells |
| title_fullStr | Experimental study on the optimization of ANM33 release in foam cells |
| title_full_unstemmed | Experimental study on the optimization of ANM33 release in foam cells |
| title_short | Experimental study on the optimization of ANM33 release in foam cells |
| title_sort | experimental study on the optimization of anm33 release in foam cells |
| topic | drug delivery dual targeting foam cells atherosclerosis |
| url | https://doi.org/10.1515/biol-2022-0564 |
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