Association of clinical outcomes and the predictive value of T lymphocyte subsets within colorectal cancer patients
IntroductionTumor immunity is a hot topic in tumor research today, and human immunity is closely related to tumor progression. T lymphocyte is an important component of human immune system, and the changes in their subsets may influence the progression of colorectal cancer (CRC) to some extent. This...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Surgery |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsurg.2023.1102545/full |
| _version_ | 1797834751863685120 |
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| author | Chaofeng Yuan Jiannan Huang Haitao Li Rongnan Zhai Jinjing Zhai Xuedong Fang Yuanyu Wu |
| author_facet | Chaofeng Yuan Jiannan Huang Haitao Li Rongnan Zhai Jinjing Zhai Xuedong Fang Yuanyu Wu |
| author_sort | Chaofeng Yuan |
| collection | DOAJ |
| description | IntroductionTumor immunity is a hot topic in tumor research today, and human immunity is closely related to tumor progression. T lymphocyte is an important component of human immune system, and the changes in their subsets may influence the progression of colorectal cancer (CRC) to some extent. This clinical study systematically describes and analyzes the association of CD4+ and CD8+ T-lymphocyte content and CD4+/CD8+ T-lymphocyte ratio with CRC differentiation, clinical pathological stage, Ki67 expression, T-stage, N-stage, carcinoembryonic antigen (CEA) content, nerve and vascular infiltration, and other clinical features, as well as preoperative and postoperative trends. Furthermore, a predictive model is constructed to evaluate the predictive value of T-lymphocyte subsets for CRC clinical features.MethodsStrict inclusion and exclusion criterion were formulated to screen patients, preoperative and postoperative flow cytometry and postoperative pathology reports from standard laparoscopic surgery were assessed. PASS and SPSS software, R packages were invoked to calculate and analyze.ResultsWe found that a high CD4+ T-lymphocyte content in peripheral blood and a high CD4+/CD8+ ratio were associated with better tumor differentiation, an earlier clinical pathological stage, lower Ki67 expression, shallower tumor infiltration, a smaller number of lymph node metastases, a lower CEA content, and a lower likelihood of nerve or vascular infiltration (P < 0.05). However, a high CD8+ T-lymphocyte content indicated an unpromising clinical profile. After effective surgical treatment, the CD4+ T-lymphocyte content and CD4+/CD8+ ratio increased significantly (P < 0.05), while the CD8+ T-lymphocyte content decreased significantly (P < 0.05). Further, we comprehensively compared the merits of CD4+ T-lymphocyte content, CD8+ T-lymphocyte content, and CD4+/CD8+ ratio in predicting the clinical features of CRC. We then combined the CD4+ and CD8+ T-lymphocyte content to build models and predict major clinical characteristics. We compared these models with the CD4+/CD8+ ratio to explore their advantages and disadvantages in predicting the clinical features of CRC.DiscussionOur results provide a theoretical basis for the future screening of effective markers in reflecting and predicting the progression of CRC. Changes in T lymphocyte subsets affect the progression of CRC to a certain extent, while their changes also reflect variations in the human immune system. |
| first_indexed | 2024-04-09T14:42:30Z |
| format | Article |
| id | doaj.art-967e424cf48e42808694bbcba26241fb |
| institution | Directory Open Access Journal |
| issn | 2296-875X |
| language | English |
| last_indexed | 2024-04-09T14:42:30Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Surgery |
| spelling | doaj.art-967e424cf48e42808694bbcba26241fb2023-05-03T04:55:08ZengFrontiers Media S.A.Frontiers in Surgery2296-875X2023-05-011010.3389/fsurg.2023.11025451102545Association of clinical outcomes and the predictive value of T lymphocyte subsets within colorectal cancer patientsChaofeng Yuan0Jiannan Huang1Haitao Li2Rongnan Zhai3Jinjing Zhai4Xuedong Fang5Yuanyu Wu6Department of Gastrointestinal Colorectal Surgery, China-Japan Union Hospital of Jilin University, Changchun, ChinaDepartment of Gastrointestinal Colorectal Surgery, China-Japan Union Hospital of Jilin University, Changchun, ChinaDepartment of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, ChinaDepartment of Gastrointestinal Colorectal Surgery, China-Japan Union Hospital of Jilin University, Changchun, ChinaDepartment of Gastrointestinal Colorectal Surgery, China-Japan Union Hospital of Jilin University, Changchun, ChinaDepartment of Gastrointestinal Colorectal Surgery, China-Japan Union Hospital of Jilin University, Changchun, ChinaDepartment of Gastrointestinal Colorectal Surgery, China-Japan Union Hospital of Jilin University, Changchun, ChinaIntroductionTumor immunity is a hot topic in tumor research today, and human immunity is closely related to tumor progression. T lymphocyte is an important component of human immune system, and the changes in their subsets may influence the progression of colorectal cancer (CRC) to some extent. This clinical study systematically describes and analyzes the association of CD4+ and CD8+ T-lymphocyte content and CD4+/CD8+ T-lymphocyte ratio with CRC differentiation, clinical pathological stage, Ki67 expression, T-stage, N-stage, carcinoembryonic antigen (CEA) content, nerve and vascular infiltration, and other clinical features, as well as preoperative and postoperative trends. Furthermore, a predictive model is constructed to evaluate the predictive value of T-lymphocyte subsets for CRC clinical features.MethodsStrict inclusion and exclusion criterion were formulated to screen patients, preoperative and postoperative flow cytometry and postoperative pathology reports from standard laparoscopic surgery were assessed. PASS and SPSS software, R packages were invoked to calculate and analyze.ResultsWe found that a high CD4+ T-lymphocyte content in peripheral blood and a high CD4+/CD8+ ratio were associated with better tumor differentiation, an earlier clinical pathological stage, lower Ki67 expression, shallower tumor infiltration, a smaller number of lymph node metastases, a lower CEA content, and a lower likelihood of nerve or vascular infiltration (P < 0.05). However, a high CD8+ T-lymphocyte content indicated an unpromising clinical profile. After effective surgical treatment, the CD4+ T-lymphocyte content and CD4+/CD8+ ratio increased significantly (P < 0.05), while the CD8+ T-lymphocyte content decreased significantly (P < 0.05). Further, we comprehensively compared the merits of CD4+ T-lymphocyte content, CD8+ T-lymphocyte content, and CD4+/CD8+ ratio in predicting the clinical features of CRC. We then combined the CD4+ and CD8+ T-lymphocyte content to build models and predict major clinical characteristics. We compared these models with the CD4+/CD8+ ratio to explore their advantages and disadvantages in predicting the clinical features of CRC.DiscussionOur results provide a theoretical basis for the future screening of effective markers in reflecting and predicting the progression of CRC. Changes in T lymphocyte subsets affect the progression of CRC to a certain extent, while their changes also reflect variations in the human immune system.https://www.frontiersin.org/articles/10.3389/fsurg.2023.1102545/fullcolorectal cancerflow cytometrylaparoscopytumor markersimmunity |
| spellingShingle | Chaofeng Yuan Jiannan Huang Haitao Li Rongnan Zhai Jinjing Zhai Xuedong Fang Yuanyu Wu Association of clinical outcomes and the predictive value of T lymphocyte subsets within colorectal cancer patients Frontiers in Surgery colorectal cancer flow cytometry laparoscopy tumor markers immunity |
| title | Association of clinical outcomes and the predictive value of T lymphocyte subsets within colorectal cancer patients |
| title_full | Association of clinical outcomes and the predictive value of T lymphocyte subsets within colorectal cancer patients |
| title_fullStr | Association of clinical outcomes and the predictive value of T lymphocyte subsets within colorectal cancer patients |
| title_full_unstemmed | Association of clinical outcomes and the predictive value of T lymphocyte subsets within colorectal cancer patients |
| title_short | Association of clinical outcomes and the predictive value of T lymphocyte subsets within colorectal cancer patients |
| title_sort | association of clinical outcomes and the predictive value of t lymphocyte subsets within colorectal cancer patients |
| topic | colorectal cancer flow cytometry laparoscopy tumor markers immunity |
| url | https://www.frontiersin.org/articles/10.3389/fsurg.2023.1102545/full |
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