Computation of Pump-Leak Flux Balance in Animal Cells
Background/Aims: Many vital processes in animal cells depend on monovalent ion transport across the plasma membrane via specific pathways. Their operation is described by a set of nonlinear and transcendental equations that cannot be solved analytically. Previous computations had been optimized for...
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2014-11-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | http://www.karger.com/Article/FullText/366382 |
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| author | Igor A. Vereninov Valentina E. Yurinskaya Michael A. Model Florian Lang Alexey A. Vereninov |
| author_facet | Igor A. Vereninov Valentina E. Yurinskaya Michael A. Model Florian Lang Alexey A. Vereninov |
| author_sort | Igor A. Vereninov |
| collection | DOAJ |
| description | Background/Aims: Many vital processes in animal cells depend on monovalent ion transport across the plasma membrane via specific pathways. Their operation is described by a set of nonlinear and transcendental equations that cannot be solved analytically. Previous computations had been optimized for certain cell types and included parameters whose experimental determination can be challenging. Methods: We have developed a simpler and a more universal computational approach by using fewer kinetic parameters derived from the data related to cell balanced state. A file is provided for calculating unidirectional Na+, K+, and Cl- fluxes via all major pathways (i.e. the Na/K pump, Na+, K+, Cl- channels, and NKCC, KC and NC cotransporters) under a balanced state and during transient processes. Results: The data on the Na+, K+, and Cl- distribution and the pump flux of K+ (Rb+) are obtained on U937 cells before and after inhibiting the pump with ouabain. There was a good match between the results of calculations and the experimentally measured dynamics of ion redistribution caused by blocking the pump. Conclusion: The presented approach can serve as an effective tool for analyzing monovalent ion transport in the whole cell, determination of the rate coefficients for ion transfer via major pathways and studying their alteration under various conditions. |
| first_indexed | 2024-12-20T10:49:10Z |
| format | Article |
| id | doaj.art-96817779b42e44a2b43621a16e9cf145 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-20T10:49:10Z |
| publishDate | 2014-11-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-96817779b42e44a2b43621a16e9cf1452022-12-21T19:43:20ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782014-11-013451812182310.1159/000366382366382Computation of Pump-Leak Flux Balance in Animal CellsIgor A. VereninovValentina E. YurinskayaMichael A. ModelFlorian LangAlexey A. VereninovBackground/Aims: Many vital processes in animal cells depend on monovalent ion transport across the plasma membrane via specific pathways. Their operation is described by a set of nonlinear and transcendental equations that cannot be solved analytically. Previous computations had been optimized for certain cell types and included parameters whose experimental determination can be challenging. Methods: We have developed a simpler and a more universal computational approach by using fewer kinetic parameters derived from the data related to cell balanced state. A file is provided for calculating unidirectional Na+, K+, and Cl- fluxes via all major pathways (i.e. the Na/K pump, Na+, K+, Cl- channels, and NKCC, KC and NC cotransporters) under a balanced state and during transient processes. Results: The data on the Na+, K+, and Cl- distribution and the pump flux of K+ (Rb+) are obtained on U937 cells before and after inhibiting the pump with ouabain. There was a good match between the results of calculations and the experimentally measured dynamics of ion redistribution caused by blocking the pump. Conclusion: The presented approach can serve as an effective tool for analyzing monovalent ion transport in the whole cell, determination of the rate coefficients for ion transfer via major pathways and studying their alteration under various conditions.http://www.karger.com/Article/FullText/366382ChannelsIon fluxesCell ion balanceCell water balanceIon transportCotransportSodium pump |
| spellingShingle | Igor A. Vereninov Valentina E. Yurinskaya Michael A. Model Florian Lang Alexey A. Vereninov Computation of Pump-Leak Flux Balance in Animal Cells Cellular Physiology and Biochemistry Channels Ion fluxes Cell ion balance Cell water balance Ion transport Cotransport Sodium pump |
| title | Computation of Pump-Leak Flux Balance in Animal Cells |
| title_full | Computation of Pump-Leak Flux Balance in Animal Cells |
| title_fullStr | Computation of Pump-Leak Flux Balance in Animal Cells |
| title_full_unstemmed | Computation of Pump-Leak Flux Balance in Animal Cells |
| title_short | Computation of Pump-Leak Flux Balance in Animal Cells |
| title_sort | computation of pump leak flux balance in animal cells |
| topic | Channels Ion fluxes Cell ion balance Cell water balance Ion transport Cotransport Sodium pump |
| url | http://www.karger.com/Article/FullText/366382 |
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