Microencapsulation in alginate and chitosan microgels to enhance viability of Bifidobacterium longum for oral delivery
Probiotic microorganisms are incorporated into a wide variety of foods, supplements, and pharmaceuticals to promote human health and wellness. However, maintaining bacterial cell viability during storage and gastrointestinal transit remains a challenge. Encapsulation of bifidobacteria within food-gr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-04-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00494/full |
| _version_ | 1819291776921370624 |
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| author | Timothy W. Yeung Elif F. Üçok Kendra A. Tiani Kendra A. Tiani D. Julian McClements D. Julian McClements David A. Sela David A. Sela David A. Sela |
| author_facet | Timothy W. Yeung Elif F. Üçok Kendra A. Tiani Kendra A. Tiani D. Julian McClements D. Julian McClements David A. Sela David A. Sela David A. Sela |
| author_sort | Timothy W. Yeung |
| collection | DOAJ |
| description | Probiotic microorganisms are incorporated into a wide variety of foods, supplements, and pharmaceuticals to promote human health and wellness. However, maintaining bacterial cell viability during storage and gastrointestinal transit remains a challenge. Encapsulation of bifidobacteria within food-grade hydrogel particles potentially mitigates their sensitivity to environmental stresses. In this study, Bifidobacterium longum subspecies and strains were encapsulated in core-shell microgels consisting of an alginate core and a microgel shell. Encapsulated obligate anaerobes Bifidobacterium longum subsp. infantis and Bifidobacterium longum subsp. longum exhibited differences in viability in a strain-dependent manner, without a discernable relationship to subspecies lineage. This includes viability under aerobic storage conditions and modeled gastrointestinal tract conditions. Coating alginate microgels with chitosan did not improve viability compared to cells encapsulated in alginate microgels alone, suggesting that modifying the surface charge alone does not enhance delivery. Thus hydrogel beads have great potential for improving the stability and efficacy of bifidobacterial probiotics in various nutritional interventions. |
| first_indexed | 2024-12-24T03:44:01Z |
| format | Article |
| id | doaj.art-96833850859842dc833fb505b791443c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-24T03:44:01Z |
| publishDate | 2016-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-96833850859842dc833fb505b791443c2022-12-21T17:16:46ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-04-01710.3389/fmicb.2016.00494194860Microencapsulation in alginate and chitosan microgels to enhance viability of Bifidobacterium longum for oral deliveryTimothy W. Yeung0Elif F. Üçok1Kendra A. Tiani2Kendra A. Tiani3D. Julian McClements4D. Julian McClements5David A. Sela6David A. Sela7David A. Sela8University of MassachusettsUniversity of MassachusettsUniversity of MassachusettsUniversity of MassachusettsUniversity of MassachusettsUniversity of MassachusettsUniversity of MassachusettsUniversity of MassachusettsUniversity of Massachusetts Medical SchoolProbiotic microorganisms are incorporated into a wide variety of foods, supplements, and pharmaceuticals to promote human health and wellness. However, maintaining bacterial cell viability during storage and gastrointestinal transit remains a challenge. Encapsulation of bifidobacteria within food-grade hydrogel particles potentially mitigates their sensitivity to environmental stresses. In this study, Bifidobacterium longum subspecies and strains were encapsulated in core-shell microgels consisting of an alginate core and a microgel shell. Encapsulated obligate anaerobes Bifidobacterium longum subsp. infantis and Bifidobacterium longum subsp. longum exhibited differences in viability in a strain-dependent manner, without a discernable relationship to subspecies lineage. This includes viability under aerobic storage conditions and modeled gastrointestinal tract conditions. Coating alginate microgels with chitosan did not improve viability compared to cells encapsulated in alginate microgels alone, suggesting that modifying the surface charge alone does not enhance delivery. Thus hydrogel beads have great potential for improving the stability and efficacy of bifidobacterial probiotics in various nutritional interventions.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00494/fullProbioticsbifidobacteriamicroencapsulationoral deliverySimulated digestion |
| spellingShingle | Timothy W. Yeung Elif F. Üçok Kendra A. Tiani Kendra A. Tiani D. Julian McClements D. Julian McClements David A. Sela David A. Sela David A. Sela Microencapsulation in alginate and chitosan microgels to enhance viability of Bifidobacterium longum for oral delivery Frontiers in Microbiology Probiotics bifidobacteria microencapsulation oral delivery Simulated digestion |
| title | Microencapsulation in alginate and chitosan microgels to enhance viability of Bifidobacterium longum for oral delivery |
| title_full | Microencapsulation in alginate and chitosan microgels to enhance viability of Bifidobacterium longum for oral delivery |
| title_fullStr | Microencapsulation in alginate and chitosan microgels to enhance viability of Bifidobacterium longum for oral delivery |
| title_full_unstemmed | Microencapsulation in alginate and chitosan microgels to enhance viability of Bifidobacterium longum for oral delivery |
| title_short | Microencapsulation in alginate and chitosan microgels to enhance viability of Bifidobacterium longum for oral delivery |
| title_sort | microencapsulation in alginate and chitosan microgels to enhance viability of bifidobacterium longum for oral delivery |
| topic | Probiotics bifidobacteria microencapsulation oral delivery Simulated digestion |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00494/full |
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