Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential
Obese sarcopenia is associated with palmitic acid (PA), an abundant circulating saturated fatty acid. This study examined a non-cytotoxic concentration of PA to provide mechanistic insights into PA-impaired skeletal myogenesis and potential medicinal and dietary interventions through edible brown se...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464621004813 |
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| author | Thilina U. Jayawardena D.P. Nagahawatta Yu-An Lu Hye-Won Yang Jun-Geon Je Seo-Young Kim You-Jin Jeon |
| author_facet | Thilina U. Jayawardena D.P. Nagahawatta Yu-An Lu Hye-Won Yang Jun-Geon Je Seo-Young Kim You-Jin Jeon |
| author_sort | Thilina U. Jayawardena |
| collection | DOAJ |
| description | Obese sarcopenia is associated with palmitic acid (PA), an abundant circulating saturated fatty acid. This study examined a non-cytotoxic concentration of PA to provide mechanistic insights into PA-impaired skeletal myogenesis and potential medicinal and dietary interventions through edible brown seaweed, Ishige okamurae (IO). C2C12 cells were examined for myogenic markers, adipogenic factors, and regenerative capacity through growth regulators against PA interference to assess IO and purified diphloroethohydoxycarmalol (DPHC) as potential treatments. Both IO and DPHC improved myogenic marker (myogenin, MyoD, and MyHC) levels. PA down-regulated myogenic markers while improving adipogenic factors (PPARγ, c/EBPα, A-FABP), DPHC significantly arbitrated the negative effects. DPHC treatment also improved phosphorylation of the growth regulatory PI3K/Akt/mTOR axis over the adverse effects of PA. The results of this study suggested regulatory mechanisms through which the bioactive components IO and DPHC based on the sustainable utilization of I. okamurae inhibited the PA-induced impairment of skeletal myogenesis. |
| first_indexed | 2024-12-13T16:24:59Z |
| format | Article |
| id | doaj.art-96850050689a4790b35615036d0e73fb |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-13T16:24:59Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-96850050689a4790b35615036d0e73fb2022-12-21T23:38:38ZengElsevierJournal of Functional Foods1756-46462021-12-0187104832Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potentialThilina U. Jayawardena0D.P. Nagahawatta1Yu-An Lu2Hye-Won Yang3Jun-Geon Je4Seo-Young Kim5You-Jin Jeon6Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of KoreaDivision of Practical Application, Honam National Institute of Biological Resources, Gohadoan-gil, Mokpo-si, Jeollanam-do 58762, Republic of KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea; Marine Science Institute, Jeju National University, Jeju Self-Governing Province 63333, Republic of Korea; Corresponding author at: Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea.Obese sarcopenia is associated with palmitic acid (PA), an abundant circulating saturated fatty acid. This study examined a non-cytotoxic concentration of PA to provide mechanistic insights into PA-impaired skeletal myogenesis and potential medicinal and dietary interventions through edible brown seaweed, Ishige okamurae (IO). C2C12 cells were examined for myogenic markers, adipogenic factors, and regenerative capacity through growth regulators against PA interference to assess IO and purified diphloroethohydoxycarmalol (DPHC) as potential treatments. Both IO and DPHC improved myogenic marker (myogenin, MyoD, and MyHC) levels. PA down-regulated myogenic markers while improving adipogenic factors (PPARγ, c/EBPα, A-FABP), DPHC significantly arbitrated the negative effects. DPHC treatment also improved phosphorylation of the growth regulatory PI3K/Akt/mTOR axis over the adverse effects of PA. The results of this study suggested regulatory mechanisms through which the bioactive components IO and DPHC based on the sustainable utilization of I. okamurae inhibited the PA-induced impairment of skeletal myogenesis.http://www.sciencedirect.com/science/article/pii/S1756464621004813Palmitic acidSkeletal myogenesisMyogenic markersAdipogenic markersGrowth regulatorsIshige okamurae |
| spellingShingle | Thilina U. Jayawardena D.P. Nagahawatta Yu-An Lu Hye-Won Yang Jun-Geon Je Seo-Young Kim You-Jin Jeon Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential Journal of Functional Foods Palmitic acid Skeletal myogenesis Myogenic markers Adipogenic markers Growth regulators Ishige okamurae |
| title | Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential |
| title_full | Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential |
| title_fullStr | Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential |
| title_full_unstemmed | Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential |
| title_short | Ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid-impaired skeletal myogenesis and improve muscle regenerative potential |
| title_sort | ishige okamurae and diphloroethohydoxycarmalol inhibit palmitic acid impaired skeletal myogenesis and improve muscle regenerative potential |
| topic | Palmitic acid Skeletal myogenesis Myogenic markers Adipogenic markers Growth regulators Ishige okamurae |
| url | http://www.sciencedirect.com/science/article/pii/S1756464621004813 |
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