Cross-amplification of heterologous microsatellite markers in Piracanjuba
ABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental fo...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Santa Maria
2017-11-01
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| Series: | Ciência Rural |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200402&lng=en&tlng=en |
| _version_ | 1831840968357183488 |
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| author | Pedro Luiz de Castro Ricardo Pereira Ribeiro Silvio Carlos Alves dos Santos Elenice Souza dos Reis Goes Felipe Pinheiro de Souza Angela Rocio Poveda-Parra Lauro Vargas Angela Maria Urrea-Rojas Nelson Mauricio Lopera-Barrero |
| author_facet | Pedro Luiz de Castro Ricardo Pereira Ribeiro Silvio Carlos Alves dos Santos Elenice Souza dos Reis Goes Felipe Pinheiro de Souza Angela Rocio Poveda-Parra Lauro Vargas Angela Maria Urrea-Rojas Nelson Mauricio Lopera-Barrero |
| author_sort | Pedro Luiz de Castro |
| collection | DOAJ |
| description | ABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided good results for cross-amplification with B. orbignyanus, involving (BoM5 and BoM13) of Brycon opalinus, (Bh5, Bh6, Bh8, Bh13 and Bh16) of Brycon hilarii, (Bc48-10) of Brycon insignis and (Par80) of Prochilodus argenteus. Primers of Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus failed to provide amplification or provided non-specificity. Results demonstrated the possibility of using primers of different species and genera of B. orbignyanus, facilitating genetic studies on the species. |
| first_indexed | 2024-12-23T06:20:31Z |
| format | Article |
| id | doaj.art-9685f896856c4706a98ce53a7a18c7e3 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-12-23T06:20:31Z |
| publishDate | 2017-11-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-9685f896856c4706a98ce53a7a18c7e32022-12-21T17:57:12ZengUniversidade Federal de Santa MariaCiência Rural1678-45962017-11-01471210.1590/0103-8478cr20170374S0103-84782017001200402Cross-amplification of heterologous microsatellite markers in PiracanjubaPedro Luiz de CastroRicardo Pereira RibeiroSilvio Carlos Alves dos SantosElenice Souza dos Reis GoesFelipe Pinheiro de SouzaAngela Rocio Poveda-ParraLauro VargasAngela Maria Urrea-RojasNelson Mauricio Lopera-BarreroABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased in number during the last years. In this context, genetic variability studies of natural stocks and in restocking programs are fundamental for the adoption of conservation measures. Current analysis verifies the cross-amplification of heterologous primers in B. orbignyanus. Fifty-two primers of the species Brycon opalinus, Brycon hilarii, Brycon insignis, Prochilodus sp., Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus were tested. Primers with the best reproducibility were applied to a sample of 20 individuals and the genetic parameters were calculated. Nine primers provided good results for cross-amplification with B. orbignyanus, involving (BoM5 and BoM13) of Brycon opalinus, (Bh5, Bh6, Bh8, Bh13 and Bh16) of Brycon hilarii, (Bc48-10) of Brycon insignis and (Par80) of Prochilodus argenteus. Primers of Piaractus mesopotamicus, Colossoma macropomum and Oreochromis niloticus failed to provide amplification or provided non-specificity. Results demonstrated the possibility of using primers of different species and genera of B. orbignyanus, facilitating genetic studies on the species.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200402&lng=en&tlng=enBrycon orbignyanusconservationmolecular markersheterologous primers |
| spellingShingle | Pedro Luiz de Castro Ricardo Pereira Ribeiro Silvio Carlos Alves dos Santos Elenice Souza dos Reis Goes Felipe Pinheiro de Souza Angela Rocio Poveda-Parra Lauro Vargas Angela Maria Urrea-Rojas Nelson Mauricio Lopera-Barrero Cross-amplification of heterologous microsatellite markers in Piracanjuba Ciência Rural Brycon orbignyanus conservation molecular markers heterologous primers |
| title | Cross-amplification of heterologous microsatellite markers in Piracanjuba |
| title_full | Cross-amplification of heterologous microsatellite markers in Piracanjuba |
| title_fullStr | Cross-amplification of heterologous microsatellite markers in Piracanjuba |
| title_full_unstemmed | Cross-amplification of heterologous microsatellite markers in Piracanjuba |
| title_short | Cross-amplification of heterologous microsatellite markers in Piracanjuba |
| title_sort | cross amplification of heterologous microsatellite markers in piracanjuba |
| topic | Brycon orbignyanus conservation molecular markers heterologous primers |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200402&lng=en&tlng=en |
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