Isolation of Pro-Osteogenic Compounds from <i>Euptelea polyandra</i> That Reciprocally Regulate Osteoblast and Osteoclast Differentiation
Plants contain a large number of small-molecule compounds that are useful for targeting human health and in drug discovery. Healthy bone metabolism depends on the balance between bone-forming osteoblast activity and bone-resorbing osteoclast activity. In an ongoing study searching for 22 plant extra...
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/24/17479 |
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| author | Ryuichiro Suzuki Yoshiaki Shirataki Akito Tomomura Kenjiro Bandow Hiroshi Sakagami Mineko Tomomura |
| author_facet | Ryuichiro Suzuki Yoshiaki Shirataki Akito Tomomura Kenjiro Bandow Hiroshi Sakagami Mineko Tomomura |
| author_sort | Ryuichiro Suzuki |
| collection | DOAJ |
| description | Plants contain a large number of small-molecule compounds that are useful for targeting human health and in drug discovery. Healthy bone metabolism depends on the balance between bone-forming osteoblast activity and bone-resorbing osteoclast activity. In an ongoing study searching for 22 plant extracts effective against osteoporosis, we found that the crude extract of <i>Euptelea polyandra</i> Sieb. et Zucc (<i>E. polyandra</i>) had osteogenic bioactivity. In this study, we isolated two compounds, isoquercitrin (<b>1</b>) and astragalin (<b>2</b>), responsible for osteogenic bioactivity in osteoblastic MC3T3-E1 cells from the leaf of <i>E. polyandra</i> using column chromatography and the spectroscopic technique. This is the first report to isolate astragalin from <i>E. polyandra</i>. Compounds (<b>1</b>) and (<b>2</b>) promoted osteoblast differentiation by increasing alkaline phosphatase (ALP) activity and alizarin red S stain-positive calcium deposition, while simultaneously suppressing tartrate-resistant acid phosphatase (TRAP)-positive osteoclast differentiation in RAW264.7 cells at non-cytotoxic concentrations. Isoquercitrin (<b>1</b>) and astragalin (<b>2</b>) increased the expression of osteoblastic differentiation genes, Osterix, ALP, and Osteoprotegerin in the MC3T3-E1 cells, while suppressing osteoclast differentiation genes, TRAP, Cathepsin K, and MMP 9 in the RAW264.7 cells. These compounds may be ideal targets for the treatment of osteoporosis due to their dual function of promoting bone formation and inhibiting bone resorption. |
| first_indexed | 2024-03-08T20:40:59Z |
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| language | English |
| last_indexed | 2024-03-08T20:40:59Z |
| publishDate | 2023-12-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9687e507a90748cab31de01442d0f1872023-12-22T14:14:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124241747910.3390/ijms242417479Isolation of Pro-Osteogenic Compounds from <i>Euptelea polyandra</i> That Reciprocally Regulate Osteoblast and Osteoclast DifferentiationRyuichiro Suzuki0Yoshiaki Shirataki1Akito Tomomura2Kenjiro Bandow3Hiroshi Sakagami4Mineko Tomomura5Department of Pharmaceutical Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado 350-0295, Saitama, JapanDepartment of Pharmaceutical Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado 350-0295, Saitama, JapanDivision of Biochemistry, Department of Oral Biology & Tissue Engineering, Meikai University School of Dentistry, Sakado 350-0283, Saitama, JapanDivision of Biochemistry, Department of Oral Biology & Tissue Engineering, Meikai University School of Dentistry, Sakado 350-0283, Saitama, JapanMeikai University Research Institute of Odontology (M-RIO), Sakado 350-0283, Saitama, JapanDepartment of Pharmaceutical Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado 350-0295, Saitama, JapanPlants contain a large number of small-molecule compounds that are useful for targeting human health and in drug discovery. Healthy bone metabolism depends on the balance between bone-forming osteoblast activity and bone-resorbing osteoclast activity. In an ongoing study searching for 22 plant extracts effective against osteoporosis, we found that the crude extract of <i>Euptelea polyandra</i> Sieb. et Zucc (<i>E. polyandra</i>) had osteogenic bioactivity. In this study, we isolated two compounds, isoquercitrin (<b>1</b>) and astragalin (<b>2</b>), responsible for osteogenic bioactivity in osteoblastic MC3T3-E1 cells from the leaf of <i>E. polyandra</i> using column chromatography and the spectroscopic technique. This is the first report to isolate astragalin from <i>E. polyandra</i>. Compounds (<b>1</b>) and (<b>2</b>) promoted osteoblast differentiation by increasing alkaline phosphatase (ALP) activity and alizarin red S stain-positive calcium deposition, while simultaneously suppressing tartrate-resistant acid phosphatase (TRAP)-positive osteoclast differentiation in RAW264.7 cells at non-cytotoxic concentrations. Isoquercitrin (<b>1</b>) and astragalin (<b>2</b>) increased the expression of osteoblastic differentiation genes, Osterix, ALP, and Osteoprotegerin in the MC3T3-E1 cells, while suppressing osteoclast differentiation genes, TRAP, Cathepsin K, and MMP 9 in the RAW264.7 cells. These compounds may be ideal targets for the treatment of osteoporosis due to their dual function of promoting bone formation and inhibiting bone resorption.https://www.mdpi.com/1422-0067/24/24/17479<i>Euptelea polyandra</i> Sieb. et Zuccisoquercitrinastragalinosteoblastosteoclastosteoporosis |
| spellingShingle | Ryuichiro Suzuki Yoshiaki Shirataki Akito Tomomura Kenjiro Bandow Hiroshi Sakagami Mineko Tomomura Isolation of Pro-Osteogenic Compounds from <i>Euptelea polyandra</i> That Reciprocally Regulate Osteoblast and Osteoclast Differentiation International Journal of Molecular Sciences <i>Euptelea polyandra</i> Sieb. et Zucc isoquercitrin astragalin osteoblast osteoclast osteoporosis |
| title | Isolation of Pro-Osteogenic Compounds from <i>Euptelea polyandra</i> That Reciprocally Regulate Osteoblast and Osteoclast Differentiation |
| title_full | Isolation of Pro-Osteogenic Compounds from <i>Euptelea polyandra</i> That Reciprocally Regulate Osteoblast and Osteoclast Differentiation |
| title_fullStr | Isolation of Pro-Osteogenic Compounds from <i>Euptelea polyandra</i> That Reciprocally Regulate Osteoblast and Osteoclast Differentiation |
| title_full_unstemmed | Isolation of Pro-Osteogenic Compounds from <i>Euptelea polyandra</i> That Reciprocally Regulate Osteoblast and Osteoclast Differentiation |
| title_short | Isolation of Pro-Osteogenic Compounds from <i>Euptelea polyandra</i> That Reciprocally Regulate Osteoblast and Osteoclast Differentiation |
| title_sort | isolation of pro osteogenic compounds from i euptelea polyandra i that reciprocally regulate osteoblast and osteoclast differentiation |
| topic | <i>Euptelea polyandra</i> Sieb. et Zucc isoquercitrin astragalin osteoblast osteoclast osteoporosis |
| url | https://www.mdpi.com/1422-0067/24/24/17479 |
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