Investigation of the Light Intensity Effect on Growth, Molting, Hemolymph Lipid, and Antioxidant Capacity of Juvenile Swimming Crab Portunus trituberculatus
An eight-week experiment was conducted to evaluate the effects of eight light intensities (0, 0.83, 1.61, 8.17, 15.89, 31.38, 63.69, and 124.31 μmol m-2 s-1) on the survival, growth, antioxidant, molting-related and apoptosis-related gene expression of juvenile swimming crab Portunus trituberculatus...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.922021/full |
| _version_ | 1811256168164622336 |
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| author | Hanying Xu Hanying Xu Juan Dou Juan Dou Qingyang Wu Qingyang Wu Yangfang Ye Yangfang Ye Changbin Song Changkao Mu Changkao Mu Chunlin Wang Chunlin Wang Zhiming Ren Zhiming Ren Ce Shi Ce Shi Ce Shi |
| author_facet | Hanying Xu Hanying Xu Juan Dou Juan Dou Qingyang Wu Qingyang Wu Yangfang Ye Yangfang Ye Changbin Song Changkao Mu Changkao Mu Chunlin Wang Chunlin Wang Zhiming Ren Zhiming Ren Ce Shi Ce Shi Ce Shi |
| author_sort | Hanying Xu |
| collection | DOAJ |
| description | An eight-week experiment was conducted to evaluate the effects of eight light intensities (0, 0.83, 1.61, 8.17, 15.89, 31.38, 63.69, and 124.31 μmol m-2 s-1) on the survival, growth, antioxidant, molting-related and apoptosis-related gene expression of juvenile swimming crab Portunus trituberculatus. The results showed that the survival rate of crabs was the highest under the light intensities of 8.17 and 15.89 μmol m-2 s-1. Under this light intensity, the crabs had the lowest hemolymph glucose (GLU) but the highest triglyceride (TG) content. The crabs in these treatments also had the highest total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT) activities, and the lowest malondialdehyde (MDA) content. The growth performance (including weight gain and specific growth rate) and molting frequency of crabs increased with increasing light intensity. The improved growth performance and molting of 8.17 and 15.89 μmol m-2 s-1 groups were also accompanied by up-regulated of the retinoid-X receptor (rxr), ecdysone receptor (ecr), nuclear receptor E75 (e75) gene expression, and down-regulated molt-inhibiting hormone (mih) gene expression. In addition, suboptimal light intensity (0, 0.83, 63.69, and 124.31 μmol m-2 s-1) significantly up-regulated the expression of apoptosis-related genes including c-Jun N-terminal kinase (jnk), tumor suppressor p53 (p53), and B-cell lymphoma-2 (bcl-2). Taken together, the suitable light intensity for the juvenile P. trituberculatus was estimated to be 9.96 μmol m-2 s-1. |
| first_indexed | 2024-04-12T17:36:00Z |
| format | Article |
| id | doaj.art-968cc698fb1f478b8c0221076ee2880a |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-12T17:36:00Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-968cc698fb1f478b8c0221076ee2880a2022-12-22T03:22:58ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-05-01910.3389/fmars.2022.922021922021Investigation of the Light Intensity Effect on Growth, Molting, Hemolymph Lipid, and Antioxidant Capacity of Juvenile Swimming Crab Portunus trituberculatusHanying Xu0Hanying Xu1Juan Dou2Juan Dou3Qingyang Wu4Qingyang Wu5Yangfang Ye6Yangfang Ye7Changbin Song8Changkao Mu9Changkao Mu10Chunlin Wang11Chunlin Wang12Zhiming Ren13Zhiming Ren14Ce Shi15Ce Shi16Ce Shi17Key Laboratory of Aquacultural Biotechnology Ministry of Education, Ningbo University, Ningbo, ChinaCollaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo, ChinaKey Laboratory of Aquacultural Biotechnology Ministry of Education, Ningbo University, Ningbo, ChinaCollaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo, ChinaKey Laboratory of Aquacultural Biotechnology Ministry of Education, Ningbo University, Ningbo, ChinaCollaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo, ChinaKey Laboratory of Aquacultural Biotechnology Ministry of Education, Ningbo University, Ningbo, ChinaCollaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo, ChinaInstitute of Semiconductors, Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Aquacultural Biotechnology Ministry of Education, Ningbo University, Ningbo, ChinaCollaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo, ChinaKey Laboratory of Aquacultural Biotechnology Ministry of Education, Ningbo University, Ningbo, ChinaCollaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo, ChinaKey Laboratory of Aquacultural Biotechnology Ministry of Education, Ningbo University, Ningbo, ChinaCollaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo, ChinaKey Laboratory of Aquacultural Biotechnology Ministry of Education, Ningbo University, Ningbo, ChinaCollaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo, ChinaMarine Economic Research Center, Dong Hai Strategic Research Institute, Ningbo University, Ningbo University, Ningbo, ChinaAn eight-week experiment was conducted to evaluate the effects of eight light intensities (0, 0.83, 1.61, 8.17, 15.89, 31.38, 63.69, and 124.31 μmol m-2 s-1) on the survival, growth, antioxidant, molting-related and apoptosis-related gene expression of juvenile swimming crab Portunus trituberculatus. The results showed that the survival rate of crabs was the highest under the light intensities of 8.17 and 15.89 μmol m-2 s-1. Under this light intensity, the crabs had the lowest hemolymph glucose (GLU) but the highest triglyceride (TG) content. The crabs in these treatments also had the highest total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT) activities, and the lowest malondialdehyde (MDA) content. The growth performance (including weight gain and specific growth rate) and molting frequency of crabs increased with increasing light intensity. The improved growth performance and molting of 8.17 and 15.89 μmol m-2 s-1 groups were also accompanied by up-regulated of the retinoid-X receptor (rxr), ecdysone receptor (ecr), nuclear receptor E75 (e75) gene expression, and down-regulated molt-inhibiting hormone (mih) gene expression. In addition, suboptimal light intensity (0, 0.83, 63.69, and 124.31 μmol m-2 s-1) significantly up-regulated the expression of apoptosis-related genes including c-Jun N-terminal kinase (jnk), tumor suppressor p53 (p53), and B-cell lymphoma-2 (bcl-2). Taken together, the suitable light intensity for the juvenile P. trituberculatus was estimated to be 9.96 μmol m-2 s-1.https://www.frontiersin.org/articles/10.3389/fmars.2022.922021/fullLEDmoltingantioxidantapoptosisswimming crab |
| spellingShingle | Hanying Xu Hanying Xu Juan Dou Juan Dou Qingyang Wu Qingyang Wu Yangfang Ye Yangfang Ye Changbin Song Changkao Mu Changkao Mu Chunlin Wang Chunlin Wang Zhiming Ren Zhiming Ren Ce Shi Ce Shi Ce Shi Investigation of the Light Intensity Effect on Growth, Molting, Hemolymph Lipid, and Antioxidant Capacity of Juvenile Swimming Crab Portunus trituberculatus Frontiers in Marine Science LED molting antioxidant apoptosis swimming crab |
| title | Investigation of the Light Intensity Effect on Growth, Molting, Hemolymph Lipid, and Antioxidant Capacity of Juvenile Swimming Crab Portunus trituberculatus |
| title_full | Investigation of the Light Intensity Effect on Growth, Molting, Hemolymph Lipid, and Antioxidant Capacity of Juvenile Swimming Crab Portunus trituberculatus |
| title_fullStr | Investigation of the Light Intensity Effect on Growth, Molting, Hemolymph Lipid, and Antioxidant Capacity of Juvenile Swimming Crab Portunus trituberculatus |
| title_full_unstemmed | Investigation of the Light Intensity Effect on Growth, Molting, Hemolymph Lipid, and Antioxidant Capacity of Juvenile Swimming Crab Portunus trituberculatus |
| title_short | Investigation of the Light Intensity Effect on Growth, Molting, Hemolymph Lipid, and Antioxidant Capacity of Juvenile Swimming Crab Portunus trituberculatus |
| title_sort | investigation of the light intensity effect on growth molting hemolymph lipid and antioxidant capacity of juvenile swimming crab portunus trituberculatus |
| topic | LED molting antioxidant apoptosis swimming crab |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.922021/full |
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