Effect of Dioscorea Opposite Waste Supplementation on Antioxidant Capacity, Immune Response and Rumen Microbiome in Weaned Lambs
Dioscorea opposite waste (DOW) has been shown to improve the gastrointestinal microbiome, antioxidation capacity, and immune activity, indicating it is a potential feed resource to improve the physiological health and rumen function of weaned lambs. In the present study, the responses of rumen micro...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/9/3/256 |
| _version_ | 1827749835468439552 |
|---|---|
| author | Ruochen Yang Yunxia Guo Shuo Zhang Qinghong Hao Chunhui Duan Yong Wang Shoukun Ji Hui Yan Yingjie Zhang Yueqin Liu |
| author_facet | Ruochen Yang Yunxia Guo Shuo Zhang Qinghong Hao Chunhui Duan Yong Wang Shoukun Ji Hui Yan Yingjie Zhang Yueqin Liu |
| author_sort | Ruochen Yang |
| collection | DOAJ |
| description | Dioscorea opposite waste (DOW) has been shown to improve the gastrointestinal microbiome, antioxidation capacity, and immune activity, indicating it is a potential feed resource to improve the physiological health and rumen function of weaned lambs. In the present study, the responses of rumen microbiome to DOW supplementation in diet were profiled using metagenome sequencing. In addition, the potential of DOW to regulate plasma parameters in weaned lambs and its possible mechanisms were investigated. Sixty healthy male small tail Han lambs (22.68 ± 2.56 kg) were selected and equally assigned to four dietary treatments: (1) DOW-free diet (CON), (2) addition of 10% DOW diet (DOW1), (3) addition of 15% DOW diet (DOW2), and (4) addition of 20% DOW diet (DOW3). Experimental lambs were fed a corresponding diet for 62 days. Rumen microbiome and plasma parameters were determined at the end of the experiment. The results showed that dietary supplementation with DOW linearly increased the concentration of aspartate aminotransferase, alkaline phosphatase, Immunoglobulin A, Immunoglobulin M, Immunoglobulin G, Glutathione peroxidase, Superoxide dismutase, and total antioxidant capacity in the plasma of weaned lambs, but an opposite trend was observed in Interleukin-1β, Interleukin-6, tumor necrosis factor-α, and Malondialdehyde between the DOW-supplemented group and the CON group. Sequencing of rumen metagenome revealed that dietary supplementation with 20% DOW significantly affected the microbial composition and function and increased the richness and diversity of rumen microbiota and relative abundance of phylum <i>Verrucomicrobia, Planctomycetes, Fibrobacteres, Chloroflexi, Actinobacteria,</i> and <i>Acidobacteria</i> and species <i>Ruminococcaceae_bacterium, Clostridiales_bacterium_NK3B98, Clostridiales_bacterium,</i> and <i>Clostridia_bacterium</i>. It was concluded that supplementing the weaned lamb’s ration with DOW increased the immune response and antioxidant capacity in a dose-dependent manner. Meanwhile, dietary supplementation with 20% DOW modulated the composition of rumen microbiome function by increasing <i>Ruminococcaceae_bacterium</i> and <i>Clostridiales_bacterium</i> with improving the polysaccharide hydrolase activity in the rumen. |
| first_indexed | 2024-03-11T06:34:07Z |
| format | Article |
| id | doaj.art-968d4906398d4dc8a7c815e8961e776f |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T06:34:07Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-968d4906398d4dc8a7c815e8961e776f2023-11-17T11:02:09ZengMDPI AGFermentation2311-56372023-03-019325610.3390/fermentation9030256Effect of Dioscorea Opposite Waste Supplementation on Antioxidant Capacity, Immune Response and Rumen Microbiome in Weaned LambsRuochen Yang0Yunxia Guo1Shuo Zhang2Qinghong Hao3Chunhui Duan4Yong Wang5Shoukun Ji6Hui Yan7Yingjie Zhang8Yueqin Liu9College of Animal Science and Technology, Hebei Agricultural University, Lokai South Street, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Lokai South Street, Baoding 071000, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, 2 Yuan Ming Yuan West Road, Haidian District, Beijing 100193, ChinaCollege of Life Sciences, Hebei Agricultural University, Lokai South Street, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Lokai South Street, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Lokai South Street, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Lokai South Street, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Lokai South Street, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Lokai South Street, Baoding 071000, ChinaCollege of Animal Science and Technology, Hebei Agricultural University, Lokai South Street, Baoding 071000, ChinaDioscorea opposite waste (DOW) has been shown to improve the gastrointestinal microbiome, antioxidation capacity, and immune activity, indicating it is a potential feed resource to improve the physiological health and rumen function of weaned lambs. In the present study, the responses of rumen microbiome to DOW supplementation in diet were profiled using metagenome sequencing. In addition, the potential of DOW to regulate plasma parameters in weaned lambs and its possible mechanisms were investigated. Sixty healthy male small tail Han lambs (22.68 ± 2.56 kg) were selected and equally assigned to four dietary treatments: (1) DOW-free diet (CON), (2) addition of 10% DOW diet (DOW1), (3) addition of 15% DOW diet (DOW2), and (4) addition of 20% DOW diet (DOW3). Experimental lambs were fed a corresponding diet for 62 days. Rumen microbiome and plasma parameters were determined at the end of the experiment. The results showed that dietary supplementation with DOW linearly increased the concentration of aspartate aminotransferase, alkaline phosphatase, Immunoglobulin A, Immunoglobulin M, Immunoglobulin G, Glutathione peroxidase, Superoxide dismutase, and total antioxidant capacity in the plasma of weaned lambs, but an opposite trend was observed in Interleukin-1β, Interleukin-6, tumor necrosis factor-α, and Malondialdehyde between the DOW-supplemented group and the CON group. Sequencing of rumen metagenome revealed that dietary supplementation with 20% DOW significantly affected the microbial composition and function and increased the richness and diversity of rumen microbiota and relative abundance of phylum <i>Verrucomicrobia, Planctomycetes, Fibrobacteres, Chloroflexi, Actinobacteria,</i> and <i>Acidobacteria</i> and species <i>Ruminococcaceae_bacterium, Clostridiales_bacterium_NK3B98, Clostridiales_bacterium,</i> and <i>Clostridia_bacterium</i>. It was concluded that supplementing the weaned lamb’s ration with DOW increased the immune response and antioxidant capacity in a dose-dependent manner. Meanwhile, dietary supplementation with 20% DOW modulated the composition of rumen microbiome function by increasing <i>Ruminococcaceae_bacterium</i> and <i>Clostridiales_bacterium</i> with improving the polysaccharide hydrolase activity in the rumen.https://www.mdpi.com/2311-5637/9/3/256Dioscorea opposite wasteweaned lambmetagenomesrumen microbiotablood metabolites |
| spellingShingle | Ruochen Yang Yunxia Guo Shuo Zhang Qinghong Hao Chunhui Duan Yong Wang Shoukun Ji Hui Yan Yingjie Zhang Yueqin Liu Effect of Dioscorea Opposite Waste Supplementation on Antioxidant Capacity, Immune Response and Rumen Microbiome in Weaned Lambs Fermentation Dioscorea opposite waste weaned lamb metagenomes rumen microbiota blood metabolites |
| title | Effect of Dioscorea Opposite Waste Supplementation on Antioxidant Capacity, Immune Response and Rumen Microbiome in Weaned Lambs |
| title_full | Effect of Dioscorea Opposite Waste Supplementation on Antioxidant Capacity, Immune Response and Rumen Microbiome in Weaned Lambs |
| title_fullStr | Effect of Dioscorea Opposite Waste Supplementation on Antioxidant Capacity, Immune Response and Rumen Microbiome in Weaned Lambs |
| title_full_unstemmed | Effect of Dioscorea Opposite Waste Supplementation on Antioxidant Capacity, Immune Response and Rumen Microbiome in Weaned Lambs |
| title_short | Effect of Dioscorea Opposite Waste Supplementation on Antioxidant Capacity, Immune Response and Rumen Microbiome in Weaned Lambs |
| title_sort | effect of dioscorea opposite waste supplementation on antioxidant capacity immune response and rumen microbiome in weaned lambs |
| topic | Dioscorea opposite waste weaned lamb metagenomes rumen microbiota blood metabolites |
| url | https://www.mdpi.com/2311-5637/9/3/256 |
| work_keys_str_mv | AT ruochenyang effectofdioscoreaoppositewastesupplementationonantioxidantcapacityimmuneresponseandrumenmicrobiomeinweanedlambs AT yunxiaguo effectofdioscoreaoppositewastesupplementationonantioxidantcapacityimmuneresponseandrumenmicrobiomeinweanedlambs AT shuozhang effectofdioscoreaoppositewastesupplementationonantioxidantcapacityimmuneresponseandrumenmicrobiomeinweanedlambs AT qinghonghao effectofdioscoreaoppositewastesupplementationonantioxidantcapacityimmuneresponseandrumenmicrobiomeinweanedlambs AT chunhuiduan effectofdioscoreaoppositewastesupplementationonantioxidantcapacityimmuneresponseandrumenmicrobiomeinweanedlambs AT yongwang effectofdioscoreaoppositewastesupplementationonantioxidantcapacityimmuneresponseandrumenmicrobiomeinweanedlambs AT shoukunji effectofdioscoreaoppositewastesupplementationonantioxidantcapacityimmuneresponseandrumenmicrobiomeinweanedlambs AT huiyan effectofdioscoreaoppositewastesupplementationonantioxidantcapacityimmuneresponseandrumenmicrobiomeinweanedlambs AT yingjiezhang effectofdioscoreaoppositewastesupplementationonantioxidantcapacityimmuneresponseandrumenmicrobiomeinweanedlambs AT yueqinliu effectofdioscoreaoppositewastesupplementationonantioxidantcapacityimmuneresponseandrumenmicrobiomeinweanedlambs |