Polysaccharide Derived from <i>Nelumbo nucifera</i> Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells
Polysaccharides are key bioactive compounds in lotus plumule tea, but their anti-diabetes activities remain unclear. The purpose of this study was to investigate the prebiotic activities of a novel polysaccharide fraction from the <i>Nelumbo nucifera</i> lotus plumule, and to examine its...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/11/1780 |
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| author | Bao Le Pham-Thi-Ngoc Anh Seung-Hwan Yang |
| author_facet | Bao Le Pham-Thi-Ngoc Anh Seung-Hwan Yang |
| author_sort | Bao Le |
| collection | DOAJ |
| description | Polysaccharides are key bioactive compounds in lotus plumule tea, but their anti-diabetes activities remain unclear. The purpose of this study was to investigate the prebiotic activities of a novel polysaccharide fraction from the <i>Nelumbo nucifera</i> lotus plumule, and to examine its regulation of glucose metabolism in insulin-resistant HepG2 cells. The <i>N. nucifera</i> polysaccharide (NNP) was purified after discoloration, hot water extraction, ethanol precipitation, and DEAE-cellulose chromatography to obtain purified polysaccharide fractions (NNP-2). Fourier transform infrared spectroscopy was used to analyze the main structural characteristics and functional group of NNP-2. Physicochemical characterization indicated that NNP-2 had a molecular weight of 110.47 kDa and consisted of xylose, glucose, fructose, galactose, and fucose in a molar ratio of 33.4:25.7:22.0:10.5:8.1. The prebiotic activity of NNP-2 was demonstrated in vitro using <i>Lactobacillus</i> and <i>Bifidobacterium</i>. Furthermore, NNP-2 showed bioactivity against α-glucosidase (IC<sub>50</sub> = 97.32 µg/mL). High glucose-induced insulin-resistant HepG2 cells were used to study the effect of NNP-2 on glucose consumption, and the molecular mechanism of the insulin transduction pathway was studied using RT-qPCR. NNP-2 could improve insulin resistance by modulating the IRS1/PI3K/Akt pathway in insulin-resistant HepG2 cells. Our data demonstrated that the <i>Nelumbo nucifera</i> polysaccharides are potential sources for nutraceuticals, and we propose functional food developments from the bioactive polysaccharides of <i>N. nucifera</i> for the management of diabetes. |
| first_indexed | 2024-03-10T10:55:48Z |
| format | Article |
| id | doaj.art-968e10f25b0648469b162f0093315cc6 |
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| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T10:55:48Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-968e10f25b0648469b162f0093315cc62023-11-21T21:54:42ZengMDPI AGPolymers2073-43602021-05-011311178010.3390/polym13111780Polysaccharide Derived from <i>Nelumbo nucifera</i> Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 CellsBao Le0Pham-Thi-Ngoc Anh1Seung-Hwan Yang2Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, VietnamDepartment of Biotechnology, Chonnam National University, Yeosu 59626, KoreaDepartment of Biotechnology, Chonnam National University, Yeosu 59626, KoreaPolysaccharides are key bioactive compounds in lotus plumule tea, but their anti-diabetes activities remain unclear. The purpose of this study was to investigate the prebiotic activities of a novel polysaccharide fraction from the <i>Nelumbo nucifera</i> lotus plumule, and to examine its regulation of glucose metabolism in insulin-resistant HepG2 cells. The <i>N. nucifera</i> polysaccharide (NNP) was purified after discoloration, hot water extraction, ethanol precipitation, and DEAE-cellulose chromatography to obtain purified polysaccharide fractions (NNP-2). Fourier transform infrared spectroscopy was used to analyze the main structural characteristics and functional group of NNP-2. Physicochemical characterization indicated that NNP-2 had a molecular weight of 110.47 kDa and consisted of xylose, glucose, fructose, galactose, and fucose in a molar ratio of 33.4:25.7:22.0:10.5:8.1. The prebiotic activity of NNP-2 was demonstrated in vitro using <i>Lactobacillus</i> and <i>Bifidobacterium</i>. Furthermore, NNP-2 showed bioactivity against α-glucosidase (IC<sub>50</sub> = 97.32 µg/mL). High glucose-induced insulin-resistant HepG2 cells were used to study the effect of NNP-2 on glucose consumption, and the molecular mechanism of the insulin transduction pathway was studied using RT-qPCR. NNP-2 could improve insulin resistance by modulating the IRS1/PI3K/Akt pathway in insulin-resistant HepG2 cells. Our data demonstrated that the <i>Nelumbo nucifera</i> polysaccharides are potential sources for nutraceuticals, and we propose functional food developments from the bioactive polysaccharides of <i>N. nucifera</i> for the management of diabetes.https://www.mdpi.com/2073-4360/13/11/1780HepG2diabetesIRS1/PI3K/Akt<i>Nelumbo nucifera</i>polysaccharide |
| spellingShingle | Bao Le Pham-Thi-Ngoc Anh Seung-Hwan Yang Polysaccharide Derived from <i>Nelumbo nucifera</i> Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells Polymers HepG2 diabetes IRS1/PI3K/Akt <i>Nelumbo nucifera</i> polysaccharide |
| title | Polysaccharide Derived from <i>Nelumbo nucifera</i> Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells |
| title_full | Polysaccharide Derived from <i>Nelumbo nucifera</i> Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells |
| title_fullStr | Polysaccharide Derived from <i>Nelumbo nucifera</i> Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells |
| title_full_unstemmed | Polysaccharide Derived from <i>Nelumbo nucifera</i> Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells |
| title_short | Polysaccharide Derived from <i>Nelumbo nucifera</i> Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells |
| title_sort | polysaccharide derived from i nelumbo nucifera i lotus plumule shows potential prebiotic activity and ameliorates insulin resistance in hepg2 cells |
| topic | HepG2 diabetes IRS1/PI3K/Akt <i>Nelumbo nucifera</i> polysaccharide |
| url | https://www.mdpi.com/2073-4360/13/11/1780 |
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