DNA barcoding evaluation and its taxonomic implications in the species-rich genus Primula L. in China.
The genus Primula is extremely diverse in the east Himalaya-Hengduan Mountains (HHM) in China as a result of rapid radiation. In order to overcome the difficulty of morphological classification of this genus, we surveyed three plastid regions (rbcL, matK, and trnH-psbA) and two nuclear markers (ITS...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4395239?pdf=render |
| _version_ | 1818313519694610432 |
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| author | Hai-Fei Yan Yun-Jiao Liu Xiu-Feng Xie Cai-Yun Zhang Chi-Ming Hu Gang Hao Xue-Jun Ge |
| author_facet | Hai-Fei Yan Yun-Jiao Liu Xiu-Feng Xie Cai-Yun Zhang Chi-Ming Hu Gang Hao Xue-Jun Ge |
| author_sort | Hai-Fei Yan |
| collection | DOAJ |
| description | The genus Primula is extremely diverse in the east Himalaya-Hengduan Mountains (HHM) in China as a result of rapid radiation. In order to overcome the difficulty of morphological classification of this genus, we surveyed three plastid regions (rbcL, matK, and trnH-psbA) and two nuclear markers (ITS and ITS2) from 227 accessions representing 66 Primula species across 18 sections, to assess their discriminatory power as barcodes. We found that ITS alone or combined with plastid regions showed the best discrimination across different infrageneric ranks and at species level. We suggest rbcL + matK + ITS as the first choice at present to barcode Primula plants. Although the present barcoding combination performed poorly in many closely related species of Primula, it still provided many new insights into current Primula taxonomy, such as the underlying presence of cryptic species, and several potential improper taxonomic treatments. DNA barcoding is one useful technique in the integrative taxonomy of the genus Primula, but it still requires further efforts to improve its effectiveness in some taxonomically challenging groups. |
| first_indexed | 2024-12-13T08:35:02Z |
| format | Article |
| id | doaj.art-9691fe1f3f8c4f439406d7ba8e621205 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T08:35:02Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-9691fe1f3f8c4f439406d7ba8e6212052022-12-21T23:53:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e012290310.1371/journal.pone.0122903DNA barcoding evaluation and its taxonomic implications in the species-rich genus Primula L. in China.Hai-Fei YanYun-Jiao LiuXiu-Feng XieCai-Yun ZhangChi-Ming HuGang HaoXue-Jun GeThe genus Primula is extremely diverse in the east Himalaya-Hengduan Mountains (HHM) in China as a result of rapid radiation. In order to overcome the difficulty of morphological classification of this genus, we surveyed three plastid regions (rbcL, matK, and trnH-psbA) and two nuclear markers (ITS and ITS2) from 227 accessions representing 66 Primula species across 18 sections, to assess their discriminatory power as barcodes. We found that ITS alone or combined with plastid regions showed the best discrimination across different infrageneric ranks and at species level. We suggest rbcL + matK + ITS as the first choice at present to barcode Primula plants. Although the present barcoding combination performed poorly in many closely related species of Primula, it still provided many new insights into current Primula taxonomy, such as the underlying presence of cryptic species, and several potential improper taxonomic treatments. DNA barcoding is one useful technique in the integrative taxonomy of the genus Primula, but it still requires further efforts to improve its effectiveness in some taxonomically challenging groups.http://europepmc.org/articles/PMC4395239?pdf=render |
| spellingShingle | Hai-Fei Yan Yun-Jiao Liu Xiu-Feng Xie Cai-Yun Zhang Chi-Ming Hu Gang Hao Xue-Jun Ge DNA barcoding evaluation and its taxonomic implications in the species-rich genus Primula L. in China. PLoS ONE |
| title | DNA barcoding evaluation and its taxonomic implications in the species-rich genus Primula L. in China. |
| title_full | DNA barcoding evaluation and its taxonomic implications in the species-rich genus Primula L. in China. |
| title_fullStr | DNA barcoding evaluation and its taxonomic implications in the species-rich genus Primula L. in China. |
| title_full_unstemmed | DNA barcoding evaluation and its taxonomic implications in the species-rich genus Primula L. in China. |
| title_short | DNA barcoding evaluation and its taxonomic implications in the species-rich genus Primula L. in China. |
| title_sort | dna barcoding evaluation and its taxonomic implications in the species rich genus primula l in china |
| url | http://europepmc.org/articles/PMC4395239?pdf=render |
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