Cytocompatibility of titanium and poly(etheretherketone) surfaces after O2 non-thermal plasma sterilization
The sterilization of medical devices is paramount to achieve an acceptable level of sterility assurance and to prevent hospital-acquired infections. However, some medical devices cannot be sterilized by usual processes such as autoclave (AC) and gamma-ray irradiation (GI). A new non-thermal plasma (...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10468041/?tool=EBI |
| _version_ | 1797692812169314304 |
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| author | Christina Maillet Florence M. Klein Florian Le Bras Frederic Velard Christine Guillaume Sophie C. Gangloff Marie-Paule Gelle |
| author_facet | Christina Maillet Florence M. Klein Florian Le Bras Frederic Velard Christine Guillaume Sophie C. Gangloff Marie-Paule Gelle |
| author_sort | Christina Maillet |
| collection | DOAJ |
| description | The sterilization of medical devices is paramount to achieve an acceptable level of sterility assurance and to prevent hospital-acquired infections. However, some medical devices cannot be sterilized by usual processes such as autoclave (AC) and gamma-ray irradiation (GI). A new non-thermal plasma (NTP) process using sealed bag that preserves the sterile state of the devices could be used as an alternative sterilization method. The aim of the study was to assess the cytocompatibility of titanium and poly(etheretherketone) (PEEK) surfaces after O2-NTP sterilization compared to GI and AC. MG-63 osteoblast-like cells were seeded on titanium (TA6V) and PEEK disks sterilized by AC, GI and O2-NTP. The cells’ viability and proliferation, determined by WST-1 and DNA quantification respectively, were enhanced whatever the material types from 3 to 10 days. When seeded on titanium, MG-63 cells showed a higher viability and proliferation after GI and O2-NTP treatment compared to AC treatment. When cultured on PEEK, MG-63 cells showed a higher viability after O2-NTP treatment. No difference of proliferation was observed whatever the sterilization processes. The cell colonization of the materials’ surface was confirmed by scanning electron microscopy. Lactate dehydrogenase (LDH) assay revealed no cytotoxicity. Thus, O2-NTP led to similar cell responses to AC and GI and could be a cost-effective alternative process to the usual sterilization methods for fragile medical devices. |
| first_indexed | 2024-03-12T02:32:59Z |
| format | Article |
| id | doaj.art-969400fdfde1478e88df311ed82e46d9 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-12T02:32:59Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-969400fdfde1478e88df311ed82e46d92023-09-05T05:31:46ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01188Cytocompatibility of titanium and poly(etheretherketone) surfaces after O2 non-thermal plasma sterilizationChristina MailletFlorence M. KleinFlorian Le BrasFrederic VelardChristine GuillaumeSophie C. GangloffMarie-Paule GelleThe sterilization of medical devices is paramount to achieve an acceptable level of sterility assurance and to prevent hospital-acquired infections. However, some medical devices cannot be sterilized by usual processes such as autoclave (AC) and gamma-ray irradiation (GI). A new non-thermal plasma (NTP) process using sealed bag that preserves the sterile state of the devices could be used as an alternative sterilization method. The aim of the study was to assess the cytocompatibility of titanium and poly(etheretherketone) (PEEK) surfaces after O2-NTP sterilization compared to GI and AC. MG-63 osteoblast-like cells were seeded on titanium (TA6V) and PEEK disks sterilized by AC, GI and O2-NTP. The cells’ viability and proliferation, determined by WST-1 and DNA quantification respectively, were enhanced whatever the material types from 3 to 10 days. When seeded on titanium, MG-63 cells showed a higher viability and proliferation after GI and O2-NTP treatment compared to AC treatment. When cultured on PEEK, MG-63 cells showed a higher viability after O2-NTP treatment. No difference of proliferation was observed whatever the sterilization processes. The cell colonization of the materials’ surface was confirmed by scanning electron microscopy. Lactate dehydrogenase (LDH) assay revealed no cytotoxicity. Thus, O2-NTP led to similar cell responses to AC and GI and could be a cost-effective alternative process to the usual sterilization methods for fragile medical devices.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10468041/?tool=EBI |
| spellingShingle | Christina Maillet Florence M. Klein Florian Le Bras Frederic Velard Christine Guillaume Sophie C. Gangloff Marie-Paule Gelle Cytocompatibility of titanium and poly(etheretherketone) surfaces after O2 non-thermal plasma sterilization PLoS ONE |
| title | Cytocompatibility of titanium and poly(etheretherketone) surfaces after O2 non-thermal plasma sterilization |
| title_full | Cytocompatibility of titanium and poly(etheretherketone) surfaces after O2 non-thermal plasma sterilization |
| title_fullStr | Cytocompatibility of titanium and poly(etheretherketone) surfaces after O2 non-thermal plasma sterilization |
| title_full_unstemmed | Cytocompatibility of titanium and poly(etheretherketone) surfaces after O2 non-thermal plasma sterilization |
| title_short | Cytocompatibility of titanium and poly(etheretherketone) surfaces after O2 non-thermal plasma sterilization |
| title_sort | cytocompatibility of titanium and poly etheretherketone surfaces after o2 non thermal plasma sterilization |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10468041/?tool=EBI |
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