| Summary: | Excessive inorganic fertilizers are one of the main causes of nitrous oxide (N<sub>2</sub>O) emissions. Organic fertilizers can not only reduce the use of nitrogen (N) fertilizers by increasing soil organic matter but are also safe for the environment. The partial replacement of nitrogen (N) fertilizers with organic fertilizers can potentially reduce N<sub>2</sub>O emissions. To illuminate the best ratio for the nitrogen replacement of inorganic fertilizer, the present experiment was conducted in dryland areas of central Gansu Province and different portions of inorganic N fertilizers (200 kg ha<sup>−1</sup>); i.e., 0, 50, 37.5, 25, and 12.5% were replaced with commercial organic fertilizers to test their effects on soil physicochemical properties, the grain yield of maize, N<sub>2</sub>O emissions, and the diversity of ammonia-oxidizing archaea (AOA) and bacterial (AOB) communities. Results showed that the maximum N<sub>2</sub>O emission was obtained by 100% inorganic fertilizers and the lowest was obtained at the control (no fertilizer). Substituting inorganic fertilizers with organic manure not only reduced N<sub>2</sub>O emissions but also improved soil organic carbon content and soil moisture and typically improves grain yield and biomass. The highest reduction in N<sub>2</sub>O emissions was recorded by 50% substitution. Furthermore, 37.5% and 12.5% substitutions did not reduce the grain yield and biomass compared to 100% inorganic fertilizer, and a 37.5% substitution performed better in improving soil fertility. Organic fertilizer increased the amoA copy number of AOA but decreased that of AOB. Nitrososphaera (AOA) and Nitrosospira (AOB) were the most dominant ammonia-oxidizing communities. Structural equation modeling indicated that AOB contributes more N<sub>2</sub>O emissions than AOA and is more sensitive to changes in pH, moisture, and NO<sub>3</sub><sup>−</sup>−N, and the input of organic fertilizers may affect AOB by influencing soil physicochemical traits. In summary, replacing a reasonable proportion (37.5%) of inorganic fertilizers with organic manure improves soil fertility, reduces N<sub>2</sub>O emissions, and stabilizes production.
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