Kunming mouse strain is less susceptible to elastase‐induced abdominal aortic aneurysms
Abstract Background Porcine pancreatic elastase (PPE) is successfully used to induce abdominal aortic aneurysm (AAA) in mice. However, differences between mouse strains in susceptibility to PPE induction have been reported. Kunming mouse is one of the most frequently used strains in China but whethe...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-02-01
|
| Series: | Animal Models and Experimental Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ame2.12197 |
| _version_ | 1818986834371280896 |
|---|---|
| author | Haole Liu Kangli Tian Congcong Xia Panpan Wei Boyu Xu Weilai Fu Yankui Li Yafeng Li Liang Bai Rong Wang Weirong Wang Baohui Xu Enqi Liu Sihai Zhao |
| author_facet | Haole Liu Kangli Tian Congcong Xia Panpan Wei Boyu Xu Weilai Fu Yankui Li Yafeng Li Liang Bai Rong Wang Weirong Wang Baohui Xu Enqi Liu Sihai Zhao |
| author_sort | Haole Liu |
| collection | DOAJ |
| description | Abstract Background Porcine pancreatic elastase (PPE) is successfully used to induce abdominal aortic aneurysm (AAA) in mice. However, differences between mouse strains in susceptibility to PPE induction have been reported. Kunming mouse is one of the most frequently used strains in China but whether it is suitable for induction of AAA by PPE application remains unclear. Methods PPE infusion (1.5 units/ml) in temporary controlled aorta was performed to induce AAAs in both C57BL/6J and Kunming mice. Phosphate‐buffered saline (PBS) application was used as vehicle control. The aorta diameters of all mice were measured at days 0 and 14 after surgery to evaluate the AAA formation. Results After 14 days of PPE or PBS infusion, all mice were sacrificed and aorta tissues were collected for histological staining analysis. At the 14th day after infusion, PPE successfully induced aortic dilation in Kunming mice and typical AAA in C57BL/6J mice. The aorta diameter increased by 0.23 mm in Kunming mice after PPE infusion, while it was 0.72 mm in the C57BL/6J strain. PPE induced mild elastin degradation, smooth muscle cell (SMC) depletion and mural leucocyte infiltration in Kunming mice, but in PPE‐sensitive C57BL/6J mice, it induced total loss of SMCs, elastin disappearance and diffused infiltrated leucocytes in aortic aneurysmal segments. The effects of PPE in inducing angiogenesis and upregulating matrix metalloproteinase 2 and 9 expression in Kunming mice were also weaker than that in C57BL/6J mice. Conclusion At the reported dose of PPE, Kunming mouse is not as susceptible to AAA formation as C57BL/6J mice. The failure of PPE to induce AAA formation in Kunming mice may be associated to its inability to boost a strong inflammatory response. |
| first_indexed | 2024-12-20T18:57:05Z |
| format | Article |
| id | doaj.art-969fdb3f2e10441f87efc0c98c93d1d4 |
| institution | Directory Open Access Journal |
| issn | 2576-2095 |
| language | English |
| last_indexed | 2024-12-20T18:57:05Z |
| publishDate | 2022-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Animal Models and Experimental Medicine |
| spelling | doaj.art-969fdb3f2e10441f87efc0c98c93d1d42022-12-21T19:29:29ZengWileyAnimal Models and Experimental Medicine2576-20952022-02-0151728010.1002/ame2.12197Kunming mouse strain is less susceptible to elastase‐induced abdominal aortic aneurysmsHaole Liu0Kangli Tian1Congcong Xia2Panpan Wei3Boyu Xu4Weilai Fu5Yankui Li6Yafeng Li7Liang Bai8Rong Wang9Weirong Wang10Baohui Xu11Enqi Liu12Sihai Zhao13Institute of Cardiovascular Science School of Basic Medical Sciences Xi'an Jiaotong University Health Science Center Xi'an ChinaLaboratory Animal Center Xi'an Jiaotong University Xi'an ChinaLaboratory Animal Center Xi'an Jiaotong University Xi'an ChinaLaboratory Animal Center Xi'an Jiaotong University Xi'an ChinaLaboratory Animal Center Xi'an Jiaotong University Xi'an ChinaDepartment of Vascular Surgery The Second Hospital of Tianjin Medical University Tianjin ChinaDepartment of Vascular Surgery The Second Hospital of Tianjin Medical University Tianjin ChinaPain Rehabilitation Department of TCM Orthopedic Center Xi'an Honghui Hospital Xi'an ChinaInstitute of Cardiovascular Science School of Basic Medical Sciences Xi'an Jiaotong University Health Science Center Xi'an ChinaInstitute of Cardiovascular Science School of Basic Medical Sciences Xi'an Jiaotong University Health Science Center Xi'an ChinaInstitute of Cardiovascular Science School of Basic Medical Sciences Xi'an Jiaotong University Health Science Center Xi'an ChinaDepartment of Vascular Surgery Stanford University School of Medicine Stanford California USAInstitute of Cardiovascular Science School of Basic Medical Sciences Xi'an Jiaotong University Health Science Center Xi'an ChinaInstitute of Cardiovascular Science School of Basic Medical Sciences Xi'an Jiaotong University Health Science Center Xi'an ChinaAbstract Background Porcine pancreatic elastase (PPE) is successfully used to induce abdominal aortic aneurysm (AAA) in mice. However, differences between mouse strains in susceptibility to PPE induction have been reported. Kunming mouse is one of the most frequently used strains in China but whether it is suitable for induction of AAA by PPE application remains unclear. Methods PPE infusion (1.5 units/ml) in temporary controlled aorta was performed to induce AAAs in both C57BL/6J and Kunming mice. Phosphate‐buffered saline (PBS) application was used as vehicle control. The aorta diameters of all mice were measured at days 0 and 14 after surgery to evaluate the AAA formation. Results After 14 days of PPE or PBS infusion, all mice were sacrificed and aorta tissues were collected for histological staining analysis. At the 14th day after infusion, PPE successfully induced aortic dilation in Kunming mice and typical AAA in C57BL/6J mice. The aorta diameter increased by 0.23 mm in Kunming mice after PPE infusion, while it was 0.72 mm in the C57BL/6J strain. PPE induced mild elastin degradation, smooth muscle cell (SMC) depletion and mural leucocyte infiltration in Kunming mice, but in PPE‐sensitive C57BL/6J mice, it induced total loss of SMCs, elastin disappearance and diffused infiltrated leucocytes in aortic aneurysmal segments. The effects of PPE in inducing angiogenesis and upregulating matrix metalloproteinase 2 and 9 expression in Kunming mice were also weaker than that in C57BL/6J mice. Conclusion At the reported dose of PPE, Kunming mouse is not as susceptible to AAA formation as C57BL/6J mice. The failure of PPE to induce AAA formation in Kunming mice may be associated to its inability to boost a strong inflammatory response.https://doi.org/10.1002/ame2.12197abdominal aortic aneurysmsC57BL/6JhistologyKunmingstrain |
| spellingShingle | Haole Liu Kangli Tian Congcong Xia Panpan Wei Boyu Xu Weilai Fu Yankui Li Yafeng Li Liang Bai Rong Wang Weirong Wang Baohui Xu Enqi Liu Sihai Zhao Kunming mouse strain is less susceptible to elastase‐induced abdominal aortic aneurysms Animal Models and Experimental Medicine abdominal aortic aneurysms C57BL/6J histology Kunming strain |
| title | Kunming mouse strain is less susceptible to elastase‐induced abdominal aortic aneurysms |
| title_full | Kunming mouse strain is less susceptible to elastase‐induced abdominal aortic aneurysms |
| title_fullStr | Kunming mouse strain is less susceptible to elastase‐induced abdominal aortic aneurysms |
| title_full_unstemmed | Kunming mouse strain is less susceptible to elastase‐induced abdominal aortic aneurysms |
| title_short | Kunming mouse strain is less susceptible to elastase‐induced abdominal aortic aneurysms |
| title_sort | kunming mouse strain is less susceptible to elastase induced abdominal aortic aneurysms |
| topic | abdominal aortic aneurysms C57BL/6J histology Kunming strain |
| url | https://doi.org/10.1002/ame2.12197 |
| work_keys_str_mv | AT haoleliu kunmingmousestrainislesssusceptibletoelastaseinducedabdominalaorticaneurysms AT kanglitian kunmingmousestrainislesssusceptibletoelastaseinducedabdominalaorticaneurysms AT congcongxia kunmingmousestrainislesssusceptibletoelastaseinducedabdominalaorticaneurysms AT panpanwei kunmingmousestrainislesssusceptibletoelastaseinducedabdominalaorticaneurysms AT boyuxu kunmingmousestrainislesssusceptibletoelastaseinducedabdominalaorticaneurysms AT weilaifu kunmingmousestrainislesssusceptibletoelastaseinducedabdominalaorticaneurysms AT yankuili kunmingmousestrainislesssusceptibletoelastaseinducedabdominalaorticaneurysms AT yafengli kunmingmousestrainislesssusceptibletoelastaseinducedabdominalaorticaneurysms AT liangbai kunmingmousestrainislesssusceptibletoelastaseinducedabdominalaorticaneurysms AT rongwang kunmingmousestrainislesssusceptibletoelastaseinducedabdominalaorticaneurysms AT weirongwang kunmingmousestrainislesssusceptibletoelastaseinducedabdominalaorticaneurysms AT baohuixu kunmingmousestrainislesssusceptibletoelastaseinducedabdominalaorticaneurysms AT enqiliu kunmingmousestrainislesssusceptibletoelastaseinducedabdominalaorticaneurysms AT sihaizhao kunmingmousestrainislesssusceptibletoelastaseinducedabdominalaorticaneurysms |