Point‐of‐care multiplexed single‐cell protein secretion analysis based on tyramide signal amplification
Abstract Secreted proteins are critical mediators in various biological systems, including the immune system, nervous system, and tumor microenvironment. Understanding profound cellular functional heterogeneity in secretion requires multiplexed secreted protein analysis at the single‐cell level. How...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-02-01
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| Series: | View |
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| Online Access: | https://doi.org/10.1002/VIW.20220033 |
| _version_ | 1797901048847794176 |
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| author | Fengjiao Zhu Songnan Liu Xue Bai Xianming Liu Bingcheng Lin Yao Lu |
| author_facet | Fengjiao Zhu Songnan Liu Xue Bai Xianming Liu Bingcheng Lin Yao Lu |
| author_sort | Fengjiao Zhu |
| collection | DOAJ |
| description | Abstract Secreted proteins are critical mediators in various biological systems, including the immune system, nervous system, and tumor microenvironment. Understanding profound cellular functional heterogeneity in secretion requires multiplexed secreted protein analysis at the single‐cell level. However, most single‐cell protein secretion analysis technologies rely on bulky, expensive instruments, preventing widespread use. Herein, we proposed using a portable and low‐cost desktop scanner to detect multiplexed proteins secreted from single cells. Gold nanoparticle‐enhanced silver staining and tyramide signal amplification are combined to construct a two‐step amplification for single‐cell secretion assay detection. We used this approach to investigate the heterogeneous immune response of macrophages at the single‐cell level, which revealed that only a minority of cells could secrete 10‐fold more MCP‐1 and TNF‐α than the averaged secretion. The reported platform eliminates bulky equipment for single‐cell secretion analysis, making it affordable for basic and clinical research. |
| first_indexed | 2024-04-10T08:55:49Z |
| format | Article |
| id | doaj.art-96a0780ffbba42e4b0fb8043921c445e |
| institution | Directory Open Access Journal |
| issn | 2688-3988 2688-268X |
| language | English |
| last_indexed | 2024-04-10T08:55:49Z |
| publishDate | 2023-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | View |
| spelling | doaj.art-96a0780ffbba42e4b0fb8043921c445e2023-02-21T16:13:55ZengWileyView2688-39882688-268X2023-02-0141n/an/a10.1002/VIW.20220033Point‐of‐care multiplexed single‐cell protein secretion analysis based on tyramide signal amplificationFengjiao Zhu0Songnan Liu1Xue Bai2Xianming Liu3Bingcheng Lin4Yao Lu5Department of Biotechnology Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian ChinaDepartment of Biotechnology Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian ChinaDepartment of Biotechnology Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian ChinaDepartment of Biotechnology Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian ChinaDepartment of Biotechnology Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian ChinaDepartment of Biotechnology Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian ChinaAbstract Secreted proteins are critical mediators in various biological systems, including the immune system, nervous system, and tumor microenvironment. Understanding profound cellular functional heterogeneity in secretion requires multiplexed secreted protein analysis at the single‐cell level. However, most single‐cell protein secretion analysis technologies rely on bulky, expensive instruments, preventing widespread use. Herein, we proposed using a portable and low‐cost desktop scanner to detect multiplexed proteins secreted from single cells. Gold nanoparticle‐enhanced silver staining and tyramide signal amplification are combined to construct a two‐step amplification for single‐cell secretion assay detection. We used this approach to investigate the heterogeneous immune response of macrophages at the single‐cell level, which revealed that only a minority of cells could secrete 10‐fold more MCP‐1 and TNF‐α than the averaged secretion. The reported platform eliminates bulky equipment for single‐cell secretion analysis, making it affordable for basic and clinical research.https://doi.org/10.1002/VIW.20220033desktop scannerlow costsecretion analysissingle‐cell analysisTSA |
| spellingShingle | Fengjiao Zhu Songnan Liu Xue Bai Xianming Liu Bingcheng Lin Yao Lu Point‐of‐care multiplexed single‐cell protein secretion analysis based on tyramide signal amplification View desktop scanner low cost secretion analysis single‐cell analysis TSA |
| title | Point‐of‐care multiplexed single‐cell protein secretion analysis based on tyramide signal amplification |
| title_full | Point‐of‐care multiplexed single‐cell protein secretion analysis based on tyramide signal amplification |
| title_fullStr | Point‐of‐care multiplexed single‐cell protein secretion analysis based on tyramide signal amplification |
| title_full_unstemmed | Point‐of‐care multiplexed single‐cell protein secretion analysis based on tyramide signal amplification |
| title_short | Point‐of‐care multiplexed single‐cell protein secretion analysis based on tyramide signal amplification |
| title_sort | point of care multiplexed single cell protein secretion analysis based on tyramide signal amplification |
| topic | desktop scanner low cost secretion analysis single‐cell analysis TSA |
| url | https://doi.org/10.1002/VIW.20220033 |
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