Antiviral activity of Quercus persica L.: High efficacy and low toxicity
Background: Drug-resistant strain of Herpes simplex virus type 1 (HSV-I) has increased the interest in the use of natural substances. Aims: This study was aimed to determine minimum inhibitory concentration of hydroalchoholic extract of a traditionally used herbal plant, Quercus persica L., on HSV-...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2013-01-01
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| Series: | Advanced Biomedical Research |
| Subjects: | |
| Online Access: | http://www.advbiores.net/article.asp?issn=2277-9175;year=2013;volume=2;issue=1;spage=36;epage=36;aulast=Karimi |
| _version_ | 1811268246219784192 |
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| author | Ali Karimi Mohammad-Taghi Moradi Mojtaba Saeedi Sedigheh Asgari Mahmoud Rafieian-kopaei |
| author_facet | Ali Karimi Mohammad-Taghi Moradi Mojtaba Saeedi Sedigheh Asgari Mahmoud Rafieian-kopaei |
| author_sort | Ali Karimi |
| collection | DOAJ |
| description | Background: Drug-resistant strain of Herpes simplex virus type 1 (HSV-I) has increased the interest in the use of natural substances.
Aims: This study was aimed to determine minimum inhibitory concentration of hydroalchoholic extract of a traditionally used herbal plant, Quercus persica L., on HSV-1 replication on baby hamster kidney (BHK) cells.
Setting: The study was conducted in Shahrekord University of Medical Sciences, Iran.
Design: This was an experimental study.
Materials and Methods: BHK cells were grown in monolayer culture with Dulbecco′s modified Eagle′s medium (DMEM) supplemented with 5% fetal calf serum and plated onto 48-well culture plates. Fifty percent cytotoxic concentration (CC50%) of Q. persica L. on BHK cells was determined. Subsequently, 50% inhibitory concentration (IC50%) of the extract on replication of HSV-1 both in interacellular and exteracellular cases was assessed.
Statistical Analysis: Statistic Probit model was used for statistical analysis. The dose-dependent effect of antiviral activity of the extracts was determined by linear regression.
Results: Q. persica L. had no cytotoxic effect on this cell line. There was significant relationship between the concentration of the extract and cell death (P<0.01). IC50s of Q. persica L. on HSV-1, before and after attachment to BHK cells were 1.02 and 0.257 μg/mL, respectively. There was significant relationship between the concentration of this extract and inhibition of cytopathic effect (CPE) (P<0.05). Antioxidant capacity of the extract was 67.5%.
Conclusions: The hydroalchoholic extract of Q. persica L. is potentially an appropriate and promising anti herpetic herbal medicine. |
| first_indexed | 2024-04-12T21:18:20Z |
| format | Article |
| id | doaj.art-96a3e633fd8b420886bc0ead2dffff89 |
| institution | Directory Open Access Journal |
| issn | 2277-9175 2277-9175 |
| language | English |
| last_indexed | 2024-04-12T21:18:20Z |
| publishDate | 2013-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advanced Biomedical Research |
| spelling | doaj.art-96a3e633fd8b420886bc0ead2dffff892022-12-22T03:16:22ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752277-91752013-01-0121363610.4103/2277-9175.109722Antiviral activity of Quercus persica L.: High efficacy and low toxicityAli KarimiMohammad-Taghi MoradiMojtaba SaeediSedigheh AsgariMahmoud Rafieian-kopaeiBackground: Drug-resistant strain of Herpes simplex virus type 1 (HSV-I) has increased the interest in the use of natural substances. Aims: This study was aimed to determine minimum inhibitory concentration of hydroalchoholic extract of a traditionally used herbal plant, Quercus persica L., on HSV-1 replication on baby hamster kidney (BHK) cells. Setting: The study was conducted in Shahrekord University of Medical Sciences, Iran. Design: This was an experimental study. Materials and Methods: BHK cells were grown in monolayer culture with Dulbecco′s modified Eagle′s medium (DMEM) supplemented with 5% fetal calf serum and plated onto 48-well culture plates. Fifty percent cytotoxic concentration (CC50%) of Q. persica L. on BHK cells was determined. Subsequently, 50% inhibitory concentration (IC50%) of the extract on replication of HSV-1 both in interacellular and exteracellular cases was assessed. Statistical Analysis: Statistic Probit model was used for statistical analysis. The dose-dependent effect of antiviral activity of the extracts was determined by linear regression. Results: Q. persica L. had no cytotoxic effect on this cell line. There was significant relationship between the concentration of the extract and cell death (P<0.01). IC50s of Q. persica L. on HSV-1, before and after attachment to BHK cells were 1.02 and 0.257 μg/mL, respectively. There was significant relationship between the concentration of this extract and inhibition of cytopathic effect (CPE) (P<0.05). Antioxidant capacity of the extract was 67.5%. Conclusions: The hydroalchoholic extract of Q. persica L. is potentially an appropriate and promising anti herpetic herbal medicine.http://www.advbiores.net/article.asp?issn=2277-9175;year=2013;volume=2;issue=1;spage=36;epage=36;aulast=KarimiAcornantiviralcytopathic effectherbal medicineinhibitory concentrationmode of action |
| spellingShingle | Ali Karimi Mohammad-Taghi Moradi Mojtaba Saeedi Sedigheh Asgari Mahmoud Rafieian-kopaei Antiviral activity of Quercus persica L.: High efficacy and low toxicity Advanced Biomedical Research Acorn antiviral cytopathic effect herbal medicine inhibitory concentration mode of action |
| title | Antiviral activity of Quercus persica L.: High efficacy and low toxicity |
| title_full | Antiviral activity of Quercus persica L.: High efficacy and low toxicity |
| title_fullStr | Antiviral activity of Quercus persica L.: High efficacy and low toxicity |
| title_full_unstemmed | Antiviral activity of Quercus persica L.: High efficacy and low toxicity |
| title_short | Antiviral activity of Quercus persica L.: High efficacy and low toxicity |
| title_sort | antiviral activity of quercus persica l high efficacy and low toxicity |
| topic | Acorn antiviral cytopathic effect herbal medicine inhibitory concentration mode of action |
| url | http://www.advbiores.net/article.asp?issn=2277-9175;year=2013;volume=2;issue=1;spage=36;epage=36;aulast=Karimi |
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