Involvement of three chemosensory proteins in perception of host plant volatiles in the tea green leafhopper, Empoasca onukii
Chemosensory proteins (CSPs) can bind and transport odorant molecules, which are believed to be involved in insect chemoreception. Here, we investigated three CSPs in perception of volatiles in Empoasca onukii. Expression profiles showed that although EonuCSP4, EonuCSP 6-1 and EonuCSP6-2 were ubiqui...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.1068543/full |
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| author | Liwen Zhang Liwen Zhang Mingxian Zhao Mingxian Zhao Feiruoran Aikeremu Feiruoran Aikeremu Huoshui Huang Minsheng You Minsheng You Minsheng You Qian Zhao Qian Zhao Qian Zhao |
| author_facet | Liwen Zhang Liwen Zhang Mingxian Zhao Mingxian Zhao Feiruoran Aikeremu Feiruoran Aikeremu Huoshui Huang Minsheng You Minsheng You Minsheng You Qian Zhao Qian Zhao Qian Zhao |
| author_sort | Liwen Zhang |
| collection | DOAJ |
| description | Chemosensory proteins (CSPs) can bind and transport odorant molecules, which are believed to be involved in insect chemoreception. Here, we investigated three CSPs in perception of volatiles in Empoasca onukii. Expression profiles showed that although EonuCSP4, EonuCSP 6-1 and EonuCSP6-2 were ubiquitously expressed in heads, legs, thoraxes and abdomen, they were all highly expressed in the antennae of E. onukii. Further, fluorescence competitive binding assays revealed that EonuCSP4 and 6-1 had binding affinities for three plant volatiles, suggesting their possible involvement in the chemosensory process. Among them, EonuCSP6-1 showed relatively high binding affinities for benzaldehyde. Behavioral assays revealed that the adults of E. onukii showed a significant preference for two compounds including benzaldehyde. The predicted three-dimensional (3D) structures of these 3 CSP have the typical six α-helices, which form the hydrophobic ligand-binding pocket. We therefore suggest that Eoun6-1 might be involved in the chemoreception of the host-related volatiles for E. onukii. Our data may provide a chance of finding a suitable antagonist of alternative control strategies which block the perception of chemosensory signals in pest, preventing the food- orientation behaviors. |
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| issn | 1664-042X |
| language | English |
| last_indexed | 2024-04-11T01:05:24Z |
| publishDate | 2023-01-01 |
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| series | Frontiers in Physiology |
| spelling | doaj.art-96a51b68efc24adda14e3166cd5a3dea2023-01-04T12:49:36ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-01-011310.3389/fphys.2022.10685431068543Involvement of three chemosensory proteins in perception of host plant volatiles in the tea green leafhopper, Empoasca onukiiLiwen Zhang0Liwen Zhang1Mingxian Zhao2Mingxian Zhao3Feiruoran Aikeremu4Feiruoran Aikeremu5Huoshui Huang6Minsheng You7Minsheng You8Minsheng You9Qian Zhao10Qian Zhao11Qian Zhao12State Key Laboratory for Ecological Pest Control of Fujian/Taiwan Crops and College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaKey Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaState Key Laboratory for Ecological Pest Control of Fujian/Taiwan Crops and College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaKey Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaKey Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaInternational Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, ChinaComprehensive Technology Service Center of Quanzhou Customs, Quanzhou, ChinaState Key Laboratory for Ecological Pest Control of Fujian/Taiwan Crops and College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaKey Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaInternational Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, ChinaState Key Laboratory for Ecological Pest Control of Fujian/Taiwan Crops and College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, ChinaKey Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture and Rural Affairs, Fuzhou, ChinaInternational Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, ChinaChemosensory proteins (CSPs) can bind and transport odorant molecules, which are believed to be involved in insect chemoreception. Here, we investigated three CSPs in perception of volatiles in Empoasca onukii. Expression profiles showed that although EonuCSP4, EonuCSP 6-1 and EonuCSP6-2 were ubiquitously expressed in heads, legs, thoraxes and abdomen, they were all highly expressed in the antennae of E. onukii. Further, fluorescence competitive binding assays revealed that EonuCSP4 and 6-1 had binding affinities for three plant volatiles, suggesting their possible involvement in the chemosensory process. Among them, EonuCSP6-1 showed relatively high binding affinities for benzaldehyde. Behavioral assays revealed that the adults of E. onukii showed a significant preference for two compounds including benzaldehyde. The predicted three-dimensional (3D) structures of these 3 CSP have the typical six α-helices, which form the hydrophobic ligand-binding pocket. We therefore suggest that Eoun6-1 might be involved in the chemoreception of the host-related volatiles for E. onukii. Our data may provide a chance of finding a suitable antagonist of alternative control strategies which block the perception of chemosensory signals in pest, preventing the food- orientation behaviors.https://www.frontiersin.org/articles/10.3389/fphys.2022.1068543/fullchemosensory proteinsEmpoasca onukiiinsect chemoreceptionplant volatilesbenzaldehyde |
| spellingShingle | Liwen Zhang Liwen Zhang Mingxian Zhao Mingxian Zhao Feiruoran Aikeremu Feiruoran Aikeremu Huoshui Huang Minsheng You Minsheng You Minsheng You Qian Zhao Qian Zhao Qian Zhao Involvement of three chemosensory proteins in perception of host plant volatiles in the tea green leafhopper, Empoasca onukii Frontiers in Physiology chemosensory proteins Empoasca onukii insect chemoreception plant volatiles benzaldehyde |
| title | Involvement of three chemosensory proteins in perception of host plant volatiles in the tea green leafhopper, Empoasca onukii |
| title_full | Involvement of three chemosensory proteins in perception of host plant volatiles in the tea green leafhopper, Empoasca onukii |
| title_fullStr | Involvement of three chemosensory proteins in perception of host plant volatiles in the tea green leafhopper, Empoasca onukii |
| title_full_unstemmed | Involvement of three chemosensory proteins in perception of host plant volatiles in the tea green leafhopper, Empoasca onukii |
| title_short | Involvement of three chemosensory proteins in perception of host plant volatiles in the tea green leafhopper, Empoasca onukii |
| title_sort | involvement of three chemosensory proteins in perception of host plant volatiles in the tea green leafhopper empoasca onukii |
| topic | chemosensory proteins Empoasca onukii insect chemoreception plant volatiles benzaldehyde |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.1068543/full |
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