Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of <i>Pseudomonas</i> sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis
Bovine mastitis (BM) is the most prevalent bacterial infection in the livestock sector, affecting the dairy industry greatly. The prevention and treatment of this disease is mainly made via antibiotics, but the increasing antimicrobial resistance of pathogens has affected the efficiency of conventio...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/9/551 |
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| author | Paula Rivera Aguayo Tamara Bruna Larenas Carlos Alarcón Godoy Bernardita Cayupe Rivas Jorge González-Casanova Diana Rojas-Gómez Nelson Caro Fuentes |
| author_facet | Paula Rivera Aguayo Tamara Bruna Larenas Carlos Alarcón Godoy Bernardita Cayupe Rivas Jorge González-Casanova Diana Rojas-Gómez Nelson Caro Fuentes |
| author_sort | Paula Rivera Aguayo |
| collection | DOAJ |
| description | Bovine mastitis (BM) is the most prevalent bacterial infection in the livestock sector, affecting the dairy industry greatly. The prevention and treatment of this disease is mainly made via antibiotics, but the increasing antimicrobial resistance of pathogens has affected the efficiency of conventional drugs. <i>Pseudomonas</i> sp. is one of the pathogens involved in this infection. The therapeutic rate of cure for this environmental mastitis-causing pathogen is practically zero, regardless of treatment. Biofilm formation has been one of the main virulence mechanisms of <i>Pseudomonas</i> hence presenting resistance to antibiotic therapy. We have manufactured chitosan nanoparticles (NQo) with tripolyphosphate (TPP) using ionotropic gelation. These NQo were confronted against a <i>Pseudomonas</i> sp. strain isolated from milk samples of cows diagnosed with BM, to evaluate their antimicrobial and antibiofilm capacity. The NQo showed great antibacterial effect in the minimum inhibitory concentrations (MIC), minimum bactericidal concentration (MBC) and disk diffusion assays. Using sub lethal concentrations, NQo were tested for inhibition of biofilm formation. The results show that the nanoparticles exhibited biofilm inhibition and were capable of eradicate pre-existing mature biofilm. These findings indicate that the NQo could act as a potential alternative to antibiotic treatment of BM. |
| first_indexed | 2024-03-10T16:45:03Z |
| format | Article |
| id | doaj.art-96a7c81b0ef64b42b84a796c233f3f5f |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T16:45:03Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-96a7c81b0ef64b42b84a796c233f3f5f2023-11-20T11:41:41ZengMDPI AGAntibiotics2079-63822020-08-019955110.3390/antibiotics9090551Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of <i>Pseudomonas</i> sp. Isolated from Milk of Cows Diagnosed with Bovine MastitisPaula Rivera Aguayo0Tamara Bruna Larenas1Carlos Alarcón Godoy2Bernardita Cayupe Rivas3Jorge González-Casanova4Diana Rojas-Gómez5Nelson Caro Fuentes6Centro de Investigación Austral Biotech, Facultad de Ciencias, Universidad Santo Tomas, Avenida Ejército 146, Santiago 8370003, ChileCentro de Investigación Austral Biotech, Facultad de Ciencias, Universidad Santo Tomas, Avenida Ejército 146, Santiago 8370003, ChileCentro de Investigación Austral Biotech, Facultad de Ciencias, Universidad Santo Tomas, Avenida Ejército 146, Santiago 8370003, ChileCentro de Investigación Austral Biotech, Facultad de Ciencias, Universidad Santo Tomas, Avenida Ejército 146, Santiago 8370003, ChileInstituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Santiago 8910060, ChileEscuela de Nutrición y Dietética, Facultad de Medicina, Universidad Andres Bello, Santiago 7591538, ChileCentro de Investigación Austral Biotech, Facultad de Ciencias, Universidad Santo Tomas, Avenida Ejército 146, Santiago 8370003, ChileBovine mastitis (BM) is the most prevalent bacterial infection in the livestock sector, affecting the dairy industry greatly. The prevention and treatment of this disease is mainly made via antibiotics, but the increasing antimicrobial resistance of pathogens has affected the efficiency of conventional drugs. <i>Pseudomonas</i> sp. is one of the pathogens involved in this infection. The therapeutic rate of cure for this environmental mastitis-causing pathogen is practically zero, regardless of treatment. Biofilm formation has been one of the main virulence mechanisms of <i>Pseudomonas</i> hence presenting resistance to antibiotic therapy. We have manufactured chitosan nanoparticles (NQo) with tripolyphosphate (TPP) using ionotropic gelation. These NQo were confronted against a <i>Pseudomonas</i> sp. strain isolated from milk samples of cows diagnosed with BM, to evaluate their antimicrobial and antibiofilm capacity. The NQo showed great antibacterial effect in the minimum inhibitory concentrations (MIC), minimum bactericidal concentration (MBC) and disk diffusion assays. Using sub lethal concentrations, NQo were tested for inhibition of biofilm formation. The results show that the nanoparticles exhibited biofilm inhibition and were capable of eradicate pre-existing mature biofilm. These findings indicate that the NQo could act as a potential alternative to antibiotic treatment of BM.https://www.mdpi.com/2079-6382/9/9/551bovine mastitisantibiofilm<i>Pseudomonas</i>chitosannanoparticles |
| spellingShingle | Paula Rivera Aguayo Tamara Bruna Larenas Carlos Alarcón Godoy Bernardita Cayupe Rivas Jorge González-Casanova Diana Rojas-Gómez Nelson Caro Fuentes Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of <i>Pseudomonas</i> sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis Antibiotics bovine mastitis antibiofilm <i>Pseudomonas</i> chitosan nanoparticles |
| title | Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of <i>Pseudomonas</i> sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis |
| title_full | Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of <i>Pseudomonas</i> sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis |
| title_fullStr | Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of <i>Pseudomonas</i> sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis |
| title_full_unstemmed | Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of <i>Pseudomonas</i> sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis |
| title_short | Antimicrobial and Antibiofilm Capacity of Chitosan Nanoparticles against Wild Type Strain of <i>Pseudomonas</i> sp. Isolated from Milk of Cows Diagnosed with Bovine Mastitis |
| title_sort | antimicrobial and antibiofilm capacity of chitosan nanoparticles against wild type strain of i pseudomonas i sp isolated from milk of cows diagnosed with bovine mastitis |
| topic | bovine mastitis antibiofilm <i>Pseudomonas</i> chitosan nanoparticles |
| url | https://www.mdpi.com/2079-6382/9/9/551 |
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