GhIQD10 interacts with GhCaM7 to control cotton fiber elongation via calcium signaling
IQ67-domain (IQD) proteins function in plant defense and in organ development. The mechanisms by which they influence cotton fiber development are unknown. In the present study, GhIQD10 was expressed mainly in the transition period of cotton fiber development, and GhIQD10-overexpression lines showed...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-04-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514122002161 |
| _version_ | 1797860434348343296 |
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| author | Fan Xu Li Wang Jun Xu Qian Chen Caixia Ma Li Huang Guiming Li Ming Luo |
| author_facet | Fan Xu Li Wang Jun Xu Qian Chen Caixia Ma Li Huang Guiming Li Ming Luo |
| author_sort | Fan Xu |
| collection | DOAJ |
| description | IQ67-domain (IQD) proteins function in plant defense and in organ development. The mechanisms by which they influence cotton fiber development are unknown. In the present study, GhIQD10 was expressed mainly in the transition period of cotton fiber development, and GhIQD10-overexpression lines showed shorter fibers. GhIQD10 interacted with GhCaM7 and the interaction was inhibited by Ca2+. In in vitro ovule culture, Ca2+ rescued the shorter-fiber phenotype of GhIQD10-overexpression lines, which were insensitive to the Ca2+ channel inhibitor verapamil and the Ca2+ pool release channel blocker 2-aminoethoxydiphenyl borate. We conclude that GhIQD10 affects cotton fiber elongation via Ca2+ signaling by interacting with GhCaM7. Brassinosteroid (BR) biosynthesis and signaling genes were up-regulated in GhIQD10-overexpression lines. Fiber development in these lines was not affected by epibrassinolide or the BR biosynthesis inhibitor brassinozole, indicating that the influence of GhIQD10 on fiber elongation was not associated with BR. |
| first_indexed | 2024-04-09T21:45:47Z |
| format | Article |
| id | doaj.art-96a851c352bd4325950e5651248ceee8 |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-04-09T21:45:47Z |
| publishDate | 2023-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-96a851c352bd4325950e5651248ceee82023-03-25T05:11:07ZengKeAi Communications Co., Ltd.Crop Journal2214-51412023-04-01112447456GhIQD10 interacts with GhCaM7 to control cotton fiber elongation via calcium signalingFan Xu0Li Wang1Jun Xu2Qian Chen3Caixia Ma4Li Huang5Guiming Li6Ming Luo7Key Laboratory of Biotechnology and Crop Quality Improvement of the Ministry of Agriculture, Biotechnology Research Center, Southwest University, Chongqing 400715, ChinaZhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, Henan, ChinaKey Laboratory of Biotechnology and Crop Quality Improvement of the Ministry of Agriculture, Biotechnology Research Center, Southwest University, Chongqing 400715, ChinaAcademy of Agricultural Sciences of Southwest University, State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, Chongqing 400715, ChinaKey Laboratory of Biotechnology and Crop Quality Improvement of the Ministry of Agriculture, Biotechnology Research Center, Southwest University, Chongqing 400715, ChinaKey Laboratory of Biotechnology and Crop Quality Improvement of the Ministry of Agriculture, Biotechnology Research Center, Southwest University, Chongqing 400715, ChinaKey Laboratory of Biotechnology and Crop Quality Improvement of the Ministry of Agriculture, Biotechnology Research Center, Southwest University, Chongqing 400715, ChinaKey Laboratory of Biotechnology and Crop Quality Improvement of the Ministry of Agriculture, Biotechnology Research Center, Southwest University, Chongqing 400715, China; Corresponding author.IQ67-domain (IQD) proteins function in plant defense and in organ development. The mechanisms by which they influence cotton fiber development are unknown. In the present study, GhIQD10 was expressed mainly in the transition period of cotton fiber development, and GhIQD10-overexpression lines showed shorter fibers. GhIQD10 interacted with GhCaM7 and the interaction was inhibited by Ca2+. In in vitro ovule culture, Ca2+ rescued the shorter-fiber phenotype of GhIQD10-overexpression lines, which were insensitive to the Ca2+ channel inhibitor verapamil and the Ca2+ pool release channel blocker 2-aminoethoxydiphenyl borate. We conclude that GhIQD10 affects cotton fiber elongation via Ca2+ signaling by interacting with GhCaM7. Brassinosteroid (BR) biosynthesis and signaling genes were up-regulated in GhIQD10-overexpression lines. Fiber development in these lines was not affected by epibrassinolide or the BR biosynthesis inhibitor brassinozole, indicating that the influence of GhIQD10 on fiber elongation was not associated with BR.http://www.sciencedirect.com/science/article/pii/S2214514122002161CottonFiber elongationIQ67-domian proteinCa2+Brassinosteroid |
| spellingShingle | Fan Xu Li Wang Jun Xu Qian Chen Caixia Ma Li Huang Guiming Li Ming Luo GhIQD10 interacts with GhCaM7 to control cotton fiber elongation via calcium signaling Crop Journal Cotton Fiber elongation IQ67-domian protein Ca2+ Brassinosteroid |
| title | GhIQD10 interacts with GhCaM7 to control cotton fiber elongation via calcium signaling |
| title_full | GhIQD10 interacts with GhCaM7 to control cotton fiber elongation via calcium signaling |
| title_fullStr | GhIQD10 interacts with GhCaM7 to control cotton fiber elongation via calcium signaling |
| title_full_unstemmed | GhIQD10 interacts with GhCaM7 to control cotton fiber elongation via calcium signaling |
| title_short | GhIQD10 interacts with GhCaM7 to control cotton fiber elongation via calcium signaling |
| title_sort | ghiqd10 interacts with ghcam7 to control cotton fiber elongation via calcium signaling |
| topic | Cotton Fiber elongation IQ67-domian protein Ca2+ Brassinosteroid |
| url | http://www.sciencedirect.com/science/article/pii/S2214514122002161 |
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