Impacts of prolonged chlorpyrifos exposure on locomotion and slow-and fast- twitch skeletal muscles contractility in rats
Aim: Investigate the effect of dietary exposure to chlorpyrifos on locomotion and contraction of soleus andextensor digitorum longus (edl) involved in locomotion. Methods: Rats were fed diets containing 1 or 5 mg kg−1 of chlorpyrifos for six weeks. Locomotion has been assessed weekly using beam walk...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-01-01
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| Series: | Toxicology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750019300198 |
| _version_ | 1818358355515670528 |
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| author | Nancy Hallal Hiba El Khayat El Sabbouri Ali Salami Wiam Ramadan Hassan Khachfe Mohamed E. Moustafa Mahmoud Khalil Wissam H. Joumaa |
| author_facet | Nancy Hallal Hiba El Khayat El Sabbouri Ali Salami Wiam Ramadan Hassan Khachfe Mohamed E. Moustafa Mahmoud Khalil Wissam H. Joumaa |
| author_sort | Nancy Hallal |
| collection | DOAJ |
| description | Aim: Investigate the effect of dietary exposure to chlorpyrifos on locomotion and contraction of soleus andextensor digitorum longus (edl) involved in locomotion. Methods: Rats were fed diets containing 1 or 5 mg kg−1 of chlorpyrifos for six weeks. Locomotion has been assessed weekly using beam walking and beam balance tests. Soleus and edl were removed to study contractile properties, myofibrillar protein content and myosin heavy chain isoforms. Results: Animals treated with 5 mg kg−1 chlorpyrifos had a decrease body weight. An increase by 28% and 24% in latency time assessed by beam walking test and a decrease by 9% and 13% in the beam balance time was reported after 6 weeks of 1 and 5 chlorpyrifos exposure respectively. The contractile properties in soleus showed an increase in twitch amplitude by 25% and 63% in 1 and 5 doses respectively, without modification in the contraction time and half relaxation time. edl treated with 1 mg kg−1 showed a decrease by 35%, 42% and 22% in twitch amplitude, contraction time and half relaxation time respectively. edl treated with 5 mg kg−1 showed an increase of 23% in twitch amplitude without modification of the other parameters. These changes were associated with modification of myofibrillar protein content in all treated groups. Myosin heavy chain isoforms were altered in both skeletal muscles treated with 1 mg kg−1. Conclusion: Exposure to chlorpyrifos can alter the locomotion and produce physiological changes in a dose and muscle type related manner. Keywords: Chlorpyrifos, Contraction, Extensor digitorum longus, Locomotion, Soleus |
| first_indexed | 2024-12-13T20:27:41Z |
| format | Article |
| id | doaj.art-96ab3a0d631a4fc7b310ac389d260d7c |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-12-13T20:27:41Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-96ab3a0d631a4fc7b310ac389d260d7c2022-12-21T23:32:31ZengElsevierToxicology Reports2214-75002019-01-016598606Impacts of prolonged chlorpyrifos exposure on locomotion and slow-and fast- twitch skeletal muscles contractility in ratsNancy Hallal0Hiba El Khayat El Sabbouri1Ali Salami2Wiam Ramadan3Hassan Khachfe4Mohamed E. Moustafa5Mahmoud Khalil6Wissam H. Joumaa7Department of Biological Sciences, Faculty of Science, Beirut Arab University, Lebanon; Corresponding author.Laboratoire Rammal Hassan Rammal, Equipe de recherche PhyToxE, Faculté des Sciences (section V), Université libanaise, Nabatieh, Lebanon; PERITOX UMR-I-0, University of Picardie Jules Verne, 80025, Amiens, FranceLaboratoire Rammal Hassan Rammal, Equipe de recherche PhyToxE, Faculté des Sciences (section V), Université libanaise, Nabatieh, LebanonLaboratoire Rammal Hassan Rammal, Equipe de recherche PhyToxE, Faculté des Sciences (section V), Université libanaise, Nabatieh, Lebanon; Lebanese Institute for Biomedical Research and Application (LIBRA), International University of Beirut (BIU), Beirut, LebanonLebanese Institute for Biomedical Research and Application (LIBRA), International University of Beirut (BIU), Beirut, LebanonDepartment of Biological Sciences, Faculty of Science, Beirut Arab University, Lebanon; Department of Biochemistry, Faculty of Sciences, Alexandria University, EgyptDepartment of Biological Sciences, Faculty of Science, Beirut Arab University, LebanonLaboratoire Rammal Hassan Rammal, Equipe de recherche PhyToxE, Faculté des Sciences (section V), Université libanaise, Nabatieh, LebanonAim: Investigate the effect of dietary exposure to chlorpyrifos on locomotion and contraction of soleus andextensor digitorum longus (edl) involved in locomotion. Methods: Rats were fed diets containing 1 or 5 mg kg−1 of chlorpyrifos for six weeks. Locomotion has been assessed weekly using beam walking and beam balance tests. Soleus and edl were removed to study contractile properties, myofibrillar protein content and myosin heavy chain isoforms. Results: Animals treated with 5 mg kg−1 chlorpyrifos had a decrease body weight. An increase by 28% and 24% in latency time assessed by beam walking test and a decrease by 9% and 13% in the beam balance time was reported after 6 weeks of 1 and 5 chlorpyrifos exposure respectively. The contractile properties in soleus showed an increase in twitch amplitude by 25% and 63% in 1 and 5 doses respectively, without modification in the contraction time and half relaxation time. edl treated with 1 mg kg−1 showed a decrease by 35%, 42% and 22% in twitch amplitude, contraction time and half relaxation time respectively. edl treated with 5 mg kg−1 showed an increase of 23% in twitch amplitude without modification of the other parameters. These changes were associated with modification of myofibrillar protein content in all treated groups. Myosin heavy chain isoforms were altered in both skeletal muscles treated with 1 mg kg−1. Conclusion: Exposure to chlorpyrifos can alter the locomotion and produce physiological changes in a dose and muscle type related manner. Keywords: Chlorpyrifos, Contraction, Extensor digitorum longus, Locomotion, Soleushttp://www.sciencedirect.com/science/article/pii/S2214750019300198 |
| spellingShingle | Nancy Hallal Hiba El Khayat El Sabbouri Ali Salami Wiam Ramadan Hassan Khachfe Mohamed E. Moustafa Mahmoud Khalil Wissam H. Joumaa Impacts of prolonged chlorpyrifos exposure on locomotion and slow-and fast- twitch skeletal muscles contractility in rats Toxicology Reports |
| title | Impacts of prolonged chlorpyrifos exposure on locomotion and slow-and fast- twitch skeletal muscles contractility in rats |
| title_full | Impacts of prolonged chlorpyrifos exposure on locomotion and slow-and fast- twitch skeletal muscles contractility in rats |
| title_fullStr | Impacts of prolonged chlorpyrifos exposure on locomotion and slow-and fast- twitch skeletal muscles contractility in rats |
| title_full_unstemmed | Impacts of prolonged chlorpyrifos exposure on locomotion and slow-and fast- twitch skeletal muscles contractility in rats |
| title_short | Impacts of prolonged chlorpyrifos exposure on locomotion and slow-and fast- twitch skeletal muscles contractility in rats |
| title_sort | impacts of prolonged chlorpyrifos exposure on locomotion and slow and fast twitch skeletal muscles contractility in rats |
| url | http://www.sciencedirect.com/science/article/pii/S2214750019300198 |
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