The mosquito electrocuting trap as an exposure-free method for measuring human-biting rates by Aedes mosquito vectors
Abstract Background Entomological monitoring of Aedes vectors has largely relied on surveillance of larvae, pupae and non-host-seeking adults, which have been poorly correlated with human disease incidence. Exposure to mosquito-borne diseases can be more directly estimated using human landing catche...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-01-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-020-3887-8 |
| _version_ | 1818676092236464128 |
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| author | Leonardo D. Ortega-López Emilie Pondeville Alain Kohl Renato León Mauro Pazmiño Betancourth Floriane Almire Sergio Torres-Valencia Segundo Saldarriaga Nosrat Mirzai Heather M. Ferguson |
| author_facet | Leonardo D. Ortega-López Emilie Pondeville Alain Kohl Renato León Mauro Pazmiño Betancourth Floriane Almire Sergio Torres-Valencia Segundo Saldarriaga Nosrat Mirzai Heather M. Ferguson |
| author_sort | Leonardo D. Ortega-López |
| collection | DOAJ |
| description | Abstract Background Entomological monitoring of Aedes vectors has largely relied on surveillance of larvae, pupae and non-host-seeking adults, which have been poorly correlated with human disease incidence. Exposure to mosquito-borne diseases can be more directly estimated using human landing catches (HLC), although this method is not recommended for Aedes-borne arboviruses. We evaluated a new method previously tested with malaria vectors, the mosquito electrocuting trap (MET) as an exposure-free alternative for measuring landing rates of Aedes mosquitoes on people. Aims were to (i) compare the MET to the BG-sentinel (BGS) trap gold standard approach for sampling host-seeking Aedes vectors; and (ii) characterize the diel activity of Aedes vectors and their association with microclimatic conditions. Methods The study was conducted over 12 days in Quinindé (Ecuador) in May 2017. Mosquito sampling stations were set up in the peridomestic area of four houses. On each day of sampling, each house was allocated either a MET or a BGS trap, which were rotated amongst the four houses daily in a Latin square design. Mosquito abundance and microclimatic conditions were recorded hourly at each sampling station between 7:00–19:00 h to assess variation between vector abundance, trapping methods, and environmental conditions. All Aedes aegypti females were tested for the presence of Zika (ZIKV), dengue (DENV) and chikungunya (CHIKV) viruses. Results A higher number of Ae. aegypti females were found in MET than in BGS collections, although no statistically significant differences in mean Ae. aegypti abundance between trapping methods were found. Both trapping methods indicated female Ae. aegypti had bimodal patterns of host-seeking, being highest during early morning and late afternoon hours. Mean Ae. aegypti daily abundance was negatively associated with daily temperature. No infection by ZIKV, DENV or CHIKV was detected in any Aedes mosquitoes caught by either trapping method. Conclusion We conclude the MET performs at least as well as the BGS standard and offers the additional advantage of direct measurement of per capita human-biting rates. If detection of arboviruses can be confirmed in MET-collected Aedes in future studies, this surveillance method could provide a valuable tool for surveillance and prediction on human arboviral exposure risk. |
| first_indexed | 2024-12-17T08:37:59Z |
| format | Article |
| id | doaj.art-96ae7047a7794994ad49e2cd3f901345 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-17T08:37:59Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
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| series | Parasites & Vectors |
| spelling | doaj.art-96ae7047a7794994ad49e2cd3f9013452022-12-21T21:56:26ZengBMCParasites & Vectors1756-33052020-01-0113111410.1186/s13071-020-3887-8The mosquito electrocuting trap as an exposure-free method for measuring human-biting rates by Aedes mosquito vectorsLeonardo D. Ortega-López0Emilie Pondeville1Alain Kohl2Renato León3Mauro Pazmiño Betancourth4Floriane Almire5Sergio Torres-Valencia6Segundo Saldarriaga7Nosrat Mirzai8Heather M. Ferguson9Institute of Biodiversity, Animal Health and Comparative Medicine, University of GlasgowMRC-University of Glasgow Centre for Virus ResearchMRC-University of Glasgow Centre for Virus ResearchLaboratorio de Entomología Médica & Medicina Tropical (LEMMT), Universidad San Francisco de QuitoSchool of Engineering, University of GlasgowMRC-University of Glasgow Centre for Virus ResearchLaboratorio de Entomología Médica & Medicina Tropical (LEMMT), Universidad San Francisco de QuitoLaboratorio de Entomología Médica & Medicina Tropical (LEMMT), Universidad San Francisco de QuitoBioelectronics Unit, University of GlasgowInstitute of Biodiversity, Animal Health and Comparative Medicine, University of GlasgowAbstract Background Entomological monitoring of Aedes vectors has largely relied on surveillance of larvae, pupae and non-host-seeking adults, which have been poorly correlated with human disease incidence. Exposure to mosquito-borne diseases can be more directly estimated using human landing catches (HLC), although this method is not recommended for Aedes-borne arboviruses. We evaluated a new method previously tested with malaria vectors, the mosquito electrocuting trap (MET) as an exposure-free alternative for measuring landing rates of Aedes mosquitoes on people. Aims were to (i) compare the MET to the BG-sentinel (BGS) trap gold standard approach for sampling host-seeking Aedes vectors; and (ii) characterize the diel activity of Aedes vectors and their association with microclimatic conditions. Methods The study was conducted over 12 days in Quinindé (Ecuador) in May 2017. Mosquito sampling stations were set up in the peridomestic area of four houses. On each day of sampling, each house was allocated either a MET or a BGS trap, which were rotated amongst the four houses daily in a Latin square design. Mosquito abundance and microclimatic conditions were recorded hourly at each sampling station between 7:00–19:00 h to assess variation between vector abundance, trapping methods, and environmental conditions. All Aedes aegypti females were tested for the presence of Zika (ZIKV), dengue (DENV) and chikungunya (CHIKV) viruses. Results A higher number of Ae. aegypti females were found in MET than in BGS collections, although no statistically significant differences in mean Ae. aegypti abundance between trapping methods were found. Both trapping methods indicated female Ae. aegypti had bimodal patterns of host-seeking, being highest during early morning and late afternoon hours. Mean Ae. aegypti daily abundance was negatively associated with daily temperature. No infection by ZIKV, DENV or CHIKV was detected in any Aedes mosquitoes caught by either trapping method. Conclusion We conclude the MET performs at least as well as the BGS standard and offers the additional advantage of direct measurement of per capita human-biting rates. If detection of arboviruses can be confirmed in MET-collected Aedes in future studies, this surveillance method could provide a valuable tool for surveillance and prediction on human arboviral exposure risk.https://doi.org/10.1186/s13071-020-3887-8ZikaDengueChikungunyaArbovirusHost-seekingAedes aegypti |
| spellingShingle | Leonardo D. Ortega-López Emilie Pondeville Alain Kohl Renato León Mauro Pazmiño Betancourth Floriane Almire Sergio Torres-Valencia Segundo Saldarriaga Nosrat Mirzai Heather M. Ferguson The mosquito electrocuting trap as an exposure-free method for measuring human-biting rates by Aedes mosquito vectors Parasites & Vectors Zika Dengue Chikungunya Arbovirus Host-seeking Aedes aegypti |
| title | The mosquito electrocuting trap as an exposure-free method for measuring human-biting rates by Aedes mosquito vectors |
| title_full | The mosquito electrocuting trap as an exposure-free method for measuring human-biting rates by Aedes mosquito vectors |
| title_fullStr | The mosquito electrocuting trap as an exposure-free method for measuring human-biting rates by Aedes mosquito vectors |
| title_full_unstemmed | The mosquito electrocuting trap as an exposure-free method for measuring human-biting rates by Aedes mosquito vectors |
| title_short | The mosquito electrocuting trap as an exposure-free method for measuring human-biting rates by Aedes mosquito vectors |
| title_sort | mosquito electrocuting trap as an exposure free method for measuring human biting rates by aedes mosquito vectors |
| topic | Zika Dengue Chikungunya Arbovirus Host-seeking Aedes aegypti |
| url | https://doi.org/10.1186/s13071-020-3887-8 |
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