A Survey of Methodologies for Assessing Mast Cell Density and Activation in Patients with Functional Abdominal Pain Disorders
The aim was to assess methods utilized in assessing mast cell involvement in functional abdominal pain disorders (FAPDs), specifically to describe variability in methods utilized to assess both mast cell density and activation and determine if a consensus exists. After a literature search identified...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Gastrointestinal Disorders |
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| Online Access: | https://www.mdpi.com/2624-5647/3/4/16 |
| _version_ | 1797504434649956352 |
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| author | Hunter Friesen Meenal Singh Vivekanand Singh Jennifer V. Schurman Craig A. Friesen |
| author_facet | Hunter Friesen Meenal Singh Vivekanand Singh Jennifer V. Schurman Craig A. Friesen |
| author_sort | Hunter Friesen |
| collection | DOAJ |
| description | The aim was to assess methods utilized in assessing mast cell involvement in functional abdominal pain disorders (FAPDs), specifically to describe variability in methods utilized to assess both mast cell density and activation and determine if a consensus exists. After a literature search identified 70 manuscripts assessing mast cell density, data were extracted including FAPD diagnosis, site of biopsy, selection of microscopic fields analyzed, selection of mucosal region analyzed, method of mast cell identification, method to assess mast cell density, and if performed, method to assess mast cell activation. There appears to be some consensus favoring inmmunohistochemical stains over histochemical stains for identifying mast cells. Otherwise, considerable variability exists in methodology for assessing mast cell density and activation. Regardless of method, approximately 80% of studies found increased mast cell density and/or activation in comparison to controls with no method being superior. A wide variety of methods have been employed to assess mast cell density and activation with no well-established consensus and inadequate data to recommend specific approaches. The current methodology providing physiologic information needs to be translated to a standard methodology providing clinical information with the development of criteria establishing abnormal density and/or activation, and more importantly, predicting treatment response. |
| first_indexed | 2024-03-10T04:04:30Z |
| format | Article |
| id | doaj.art-96ae9a4015e8493dbd6193967b8c172d |
| institution | Directory Open Access Journal |
| issn | 2624-5647 |
| language | English |
| last_indexed | 2024-03-10T04:04:30Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gastrointestinal Disorders |
| spelling | doaj.art-96ae9a4015e8493dbd6193967b8c172d2023-11-23T08:27:03ZengMDPI AGGastrointestinal Disorders2624-56472021-09-013414215510.3390/gidisord3040016A Survey of Methodologies for Assessing Mast Cell Density and Activation in Patients with Functional Abdominal Pain DisordersHunter Friesen0Meenal Singh1Vivekanand Singh2Jennifer V. Schurman3Craig A. Friesen4School of Medicine, University of Kansas, Kansas City, KS 66160, USADivision of Gastroenterology, Hepatology, & Nutrition, Children’s Mercy Kansas City, 2401 Gillham Road, Kansas City, MO 64108, USADepartment of Pathology, The University of Texas Southwestern Medical Center, 1935 Medical District Drive, Dallas, TX 75235, USADivision of Gastroenterology, Hepatology, & Nutrition, Children’s Mercy Kansas City, 2401 Gillham Road, Kansas City, MO 64108, USADivision of Gastroenterology, Hepatology, & Nutrition, Children’s Mercy Kansas City, 2401 Gillham Road, Kansas City, MO 64108, USAThe aim was to assess methods utilized in assessing mast cell involvement in functional abdominal pain disorders (FAPDs), specifically to describe variability in methods utilized to assess both mast cell density and activation and determine if a consensus exists. After a literature search identified 70 manuscripts assessing mast cell density, data were extracted including FAPD diagnosis, site of biopsy, selection of microscopic fields analyzed, selection of mucosal region analyzed, method of mast cell identification, method to assess mast cell density, and if performed, method to assess mast cell activation. There appears to be some consensus favoring inmmunohistochemical stains over histochemical stains for identifying mast cells. Otherwise, considerable variability exists in methodology for assessing mast cell density and activation. Regardless of method, approximately 80% of studies found increased mast cell density and/or activation in comparison to controls with no method being superior. A wide variety of methods have been employed to assess mast cell density and activation with no well-established consensus and inadequate data to recommend specific approaches. The current methodology providing physiologic information needs to be translated to a standard methodology providing clinical information with the development of criteria establishing abnormal density and/or activation, and more importantly, predicting treatment response.https://www.mdpi.com/2624-5647/3/4/16mast cellsirritable bowel syndromefunctional dyspepsia |
| spellingShingle | Hunter Friesen Meenal Singh Vivekanand Singh Jennifer V. Schurman Craig A. Friesen A Survey of Methodologies for Assessing Mast Cell Density and Activation in Patients with Functional Abdominal Pain Disorders Gastrointestinal Disorders mast cells irritable bowel syndrome functional dyspepsia |
| title | A Survey of Methodologies for Assessing Mast Cell Density and Activation in Patients with Functional Abdominal Pain Disorders |
| title_full | A Survey of Methodologies for Assessing Mast Cell Density and Activation in Patients with Functional Abdominal Pain Disorders |
| title_fullStr | A Survey of Methodologies for Assessing Mast Cell Density and Activation in Patients with Functional Abdominal Pain Disorders |
| title_full_unstemmed | A Survey of Methodologies for Assessing Mast Cell Density and Activation in Patients with Functional Abdominal Pain Disorders |
| title_short | A Survey of Methodologies for Assessing Mast Cell Density and Activation in Patients with Functional Abdominal Pain Disorders |
| title_sort | survey of methodologies for assessing mast cell density and activation in patients with functional abdominal pain disorders |
| topic | mast cells irritable bowel syndrome functional dyspepsia |
| url | https://www.mdpi.com/2624-5647/3/4/16 |
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