Antimicrobial susceptibility of Staphylococcus sp. and Escherichia coli isolated from captive Amazonian manatee (Trichechus inunguis)
ABSTRACT: The Amazonian manatee (Trichechusinunguis) is an aquatic mammal threatened with extinction. However, few studies have investigated the pathogens in this species, which may affect both animal and human health. This study aimed to evaluate the frequency, distribution, and patterns of antimic...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
2024-03-01
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| Series: | Ciência Rural |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782024000700452&tlng=en |
| _version_ | 1827329560957419520 |
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| author | Thayanne Gabryelle Viana de Souza Rafael Gariglio Clark Xavier Jordana Almeida Santana Daniela Magalhães Drummond de Mello Vera Maria Ferreira da Silva Júlio César Câmara Rosa Henrique César Pereira Figueiredo Guilherme Campos Tavares Rodrigo Otávio Silveira Silva |
| author_facet | Thayanne Gabryelle Viana de Souza Rafael Gariglio Clark Xavier Jordana Almeida Santana Daniela Magalhães Drummond de Mello Vera Maria Ferreira da Silva Júlio César Câmara Rosa Henrique César Pereira Figueiredo Guilherme Campos Tavares Rodrigo Otávio Silveira Silva |
| author_sort | Thayanne Gabryelle Viana de Souza |
| collection | DOAJ |
| description | ABSTRACT: The Amazonian manatee (Trichechusinunguis) is an aquatic mammal threatened with extinction. However, few studies have investigated the pathogens in this species, which may affect both animal and human health. This study aimed to evaluate the frequency, distribution, and patterns of antimicrobial susceptibility of Staphylococcus spp. and Escherichiacoli colonizing the nasal and rectal cavities of Amazonian manatees kept in captivity at the National Institute for Amazonian Research (INPA) in the state of Amazonas, Brazil. Rectal and nasal swabs from 44 manatees of different ages were used in this study. The genus Staphylococcus was isolated from the nasal swabs of 32 (72.7%) animals, with two individuals harboring more than one species of Staphylococcus. S. sciuri was the most commonly isolated species. Resistance to penicillin was observed in 13 (40.6%) isolates, more frequent than the other antimicrobials tested (P = 0.01). E. coli was isolated from the rectal swabs of all animals, with phylogroup B1 being the most frequent among the strains obtained (P = 0.0008). Four isolates (6.8%) were positive for virulence factors, three of which were classified as enterotoxigenicE. coli (ETEC) and one as enteropathogenicE. coli (EPEC). To our knowledge, this is the first study to evaluate Staphylococcus spp. and E. coli in Amazonian manatee samples. This study revealed nasal colonization by Staphylococcus spp., mainly S. sciuri, and diarrheagenicE. coli isolates, including antimicrobial-resistant strains. |
| first_indexed | 2024-03-07T15:42:45Z |
| format | Article |
| id | doaj.art-96b0e0c271cc459b9ef8511619f15d28 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-03-07T15:42:45Z |
| publishDate | 2024-03-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-96b0e0c271cc459b9ef8511619f15d282024-03-05T07:37:57ZengUniversidade Federal de Santa MariaCiência Rural1678-45962024-03-0154710.1590/0103-8478cr20230140Antimicrobial susceptibility of Staphylococcus sp. and Escherichia coli isolated from captive Amazonian manatee (Trichechus inunguis)Thayanne Gabryelle Viana de Souzahttps://orcid.org/0000-0002-7611-7403Rafael Gariglio Clark Xavierhttps://orcid.org/0000-0002-4770-5134Jordana Almeida Santanahttps://orcid.org/0000-0002-0577-887XDaniela Magalhães Drummond de Mellohttps://orcid.org/0000-0001-7079-5505Vera Maria Ferreira da Silvahttps://orcid.org/0000-0002-1774-0393Júlio César Câmara Rosahttps://orcid.org/0000-0003-0189-7828Henrique César Pereira Figueiredohttps://orcid.org/0000-0002-1022-6842Guilherme Campos Tavareshttps://orcid.org/0000-0002-2735-2562Rodrigo Otávio Silveira Silvahttps://orcid.org/0000-0003-0074-2488ABSTRACT: The Amazonian manatee (Trichechusinunguis) is an aquatic mammal threatened with extinction. However, few studies have investigated the pathogens in this species, which may affect both animal and human health. This study aimed to evaluate the frequency, distribution, and patterns of antimicrobial susceptibility of Staphylococcus spp. and Escherichiacoli colonizing the nasal and rectal cavities of Amazonian manatees kept in captivity at the National Institute for Amazonian Research (INPA) in the state of Amazonas, Brazil. Rectal and nasal swabs from 44 manatees of different ages were used in this study. The genus Staphylococcus was isolated from the nasal swabs of 32 (72.7%) animals, with two individuals harboring more than one species of Staphylococcus. S. sciuri was the most commonly isolated species. Resistance to penicillin was observed in 13 (40.6%) isolates, more frequent than the other antimicrobials tested (P = 0.01). E. coli was isolated from the rectal swabs of all animals, with phylogroup B1 being the most frequent among the strains obtained (P = 0.0008). Four isolates (6.8%) were positive for virulence factors, three of which were classified as enterotoxigenicE. coli (ETEC) and one as enteropathogenicE. coli (EPEC). To our knowledge, this is the first study to evaluate Staphylococcus spp. and E. coli in Amazonian manatee samples. This study revealed nasal colonization by Staphylococcus spp., mainly S. sciuri, and diarrheagenicE. coli isolates, including antimicrobial-resistant strains.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782024000700452&tlng=enEnterobacteriaceaeTrichechidaeManatins |
| spellingShingle | Thayanne Gabryelle Viana de Souza Rafael Gariglio Clark Xavier Jordana Almeida Santana Daniela Magalhães Drummond de Mello Vera Maria Ferreira da Silva Júlio César Câmara Rosa Henrique César Pereira Figueiredo Guilherme Campos Tavares Rodrigo Otávio Silveira Silva Antimicrobial susceptibility of Staphylococcus sp. and Escherichia coli isolated from captive Amazonian manatee (Trichechus inunguis) Ciência Rural Enterobacteriaceae Trichechidae Manatins |
| title | Antimicrobial susceptibility of Staphylococcus sp. and Escherichia coli isolated from captive Amazonian manatee (Trichechus inunguis) |
| title_full | Antimicrobial susceptibility of Staphylococcus sp. and Escherichia coli isolated from captive Amazonian manatee (Trichechus inunguis) |
| title_fullStr | Antimicrobial susceptibility of Staphylococcus sp. and Escherichia coli isolated from captive Amazonian manatee (Trichechus inunguis) |
| title_full_unstemmed | Antimicrobial susceptibility of Staphylococcus sp. and Escherichia coli isolated from captive Amazonian manatee (Trichechus inunguis) |
| title_short | Antimicrobial susceptibility of Staphylococcus sp. and Escherichia coli isolated from captive Amazonian manatee (Trichechus inunguis) |
| title_sort | antimicrobial susceptibility of staphylococcus sp and escherichia coli isolated from captive amazonian manatee trichechus inunguis |
| topic | Enterobacteriaceae Trichechidae Manatins |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782024000700452&tlng=en |
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