Estudo do comportamento eletroquímico da enzima peroxidase na presença de peróxido de hidrogênio e ácido 5- aminossalicílico
The electrochemical behavior of the enzyme peroxidase (HRP) was investigated using the hydrogen peroxide as enzymatic substrate and the 5-aminosalicylic acid (5-ASA) as mediator of electrons on graphite electrodes. Several parameters were optimized, namely, the applied potential to the amperometric...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Estadual Paulista
2018-04-01
|
| Series: | Eclética Química |
| Online Access: | https://revista.iq.unesp.br/ojs/index.php/ecletica/article/view/294 |
| _version_ | 1818410294717710336 |
|---|---|
| author | Carolina Venturini Uliana Carla dos Santos Riccardi Hideko Yamanaka |
| author_facet | Carolina Venturini Uliana Carla dos Santos Riccardi Hideko Yamanaka |
| author_sort | Carolina Venturini Uliana |
| collection | DOAJ |
| description | The electrochemical behavior of the enzyme peroxidase (HRP) was investigated using the hydrogen peroxide as enzymatic substrate and the 5-aminosalicylic acid (5-ASA) as mediator of electrons on graphite electrodes. Several parameters were optimized, namely, the applied potential to the amperometric technique fixed in -0.125V, the 0.1 mol L-1 phosphate-citrate buffer at pH 5.0 as supporting electrolyte and the proportion between the 5-ASA and H2O2 in 1:7, among others. It was observed the catalysis of the oxidation reaction of the H2O2 in the presence of the enzyme HRP and 5-ASA. The oxidation product was reduced in the electrode surface, evidencing a significant increase in the intensity of the cathodic current. |
| first_indexed | 2024-12-14T10:13:14Z |
| format | Article |
| id | doaj.art-96b247e3d86d437bb2ca8fd3f1f7c473 |
| institution | Directory Open Access Journal |
| issn | 1678-4618 |
| language | English |
| last_indexed | 2024-12-14T10:13:14Z |
| publishDate | 2018-04-01 |
| publisher | Universidade Estadual Paulista |
| record_format | Article |
| series | Eclética Química |
| spelling | doaj.art-96b247e3d86d437bb2ca8fd3f1f7c4732022-12-21T23:06:57ZengUniversidade Estadual PaulistaEclética Química1678-46182018-04-01331576210.26850/1678-4618eqj.v33.1.2008.p57-62294Estudo do comportamento eletroquímico da enzima peroxidase na presença de peróxido de hidrogênio e ácido 5- aminossalicílicoCarolina Venturini Uliana0Carla dos Santos Riccardi1Hideko Yamanaka2Universidade Estadual Paulista, Instituto de Química, Campus de AraraquaraUniversidade Estadual Paulista, Instituto de Química, Campus de AraraquaraUniversidade Estadual Paulista, Instituto de Química, Campus de AraraquaraThe electrochemical behavior of the enzyme peroxidase (HRP) was investigated using the hydrogen peroxide as enzymatic substrate and the 5-aminosalicylic acid (5-ASA) as mediator of electrons on graphite electrodes. Several parameters were optimized, namely, the applied potential to the amperometric technique fixed in -0.125V, the 0.1 mol L-1 phosphate-citrate buffer at pH 5.0 as supporting electrolyte and the proportion between the 5-ASA and H2O2 in 1:7, among others. It was observed the catalysis of the oxidation reaction of the H2O2 in the presence of the enzyme HRP and 5-ASA. The oxidation product was reduced in the electrode surface, evidencing a significant increase in the intensity of the cathodic current.https://revista.iq.unesp.br/ojs/index.php/ecletica/article/view/294 |
| spellingShingle | Carolina Venturini Uliana Carla dos Santos Riccardi Hideko Yamanaka Estudo do comportamento eletroquímico da enzima peroxidase na presença de peróxido de hidrogênio e ácido 5- aminossalicílico Eclética Química |
| title | Estudo do comportamento eletroquímico da enzima peroxidase na presença de peróxido de hidrogênio e ácido 5- aminossalicílico |
| title_full | Estudo do comportamento eletroquímico da enzima peroxidase na presença de peróxido de hidrogênio e ácido 5- aminossalicílico |
| title_fullStr | Estudo do comportamento eletroquímico da enzima peroxidase na presença de peróxido de hidrogênio e ácido 5- aminossalicílico |
| title_full_unstemmed | Estudo do comportamento eletroquímico da enzima peroxidase na presença de peróxido de hidrogênio e ácido 5- aminossalicílico |
| title_short | Estudo do comportamento eletroquímico da enzima peroxidase na presença de peróxido de hidrogênio e ácido 5- aminossalicílico |
| title_sort | estudo do comportamento eletroquimico da enzima peroxidase na presenca de peroxido de hidrogenio e acido 5 aminossalicilico |
| url | https://revista.iq.unesp.br/ojs/index.php/ecletica/article/view/294 |
| work_keys_str_mv | AT carolinaventuriniuliana estudodocomportamentoeletroquimicodaenzimaperoxidasenapresencadeperoxidodehidrogenioeacido5aminossalicilico AT carladossantosriccardi estudodocomportamentoeletroquimicodaenzimaperoxidasenapresencadeperoxidodehidrogenioeacido5aminossalicilico AT hidekoyamanaka estudodocomportamentoeletroquimicodaenzimaperoxidasenapresencadeperoxidodehidrogenioeacido5aminossalicilico |