Isolation and characterization of genes functionally involved in ovarian development of the giant tiger shrimp Penaeus monodon by suppression subtractive hybridization (SSH)

Isolation and characterization of genes functionally involved in ovarian development of the giant tiger shrimp Penaeus monodon by suppression subtractive hybridization (SSH)

Suppression subtractive hybridization (SSH) libraries between cDNA in stages I (previtellogenic) and III (cortical rod) ovaries of the giant tiger shrimp (Penaeus monodon) were established. In all, 452 ESTs were unidirectionally sequenced. Sequence assembly generated 28 contigs and 201 singletons, 1...

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Bibliographic Details
Main Authors: Rachanimuk Preechaphol, Sirawut Klinbunga, Bavornlak Khamnamtongand, Piamsak Menasveta
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2010-01-01
Series:Genetics and Molecular Biology
Subjects:
EST
SSH
Penaeus monodon
ovarian development
semiquantitative RT-PCR
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400014

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