Physical–chemical properties of cell wall interface significantly correlated to the complex recalcitrance of corn straw
Abstract Background Tissue heterogeneity significantly influences the overall saccharification efficiency of plant biomass. However, the mechanisms of specific organ or tissue recalcitrance to enzymatic deconstruction are generally complicated and unclear. A multidimensional analysis of the anatomic...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-10-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-021-02047-0 |
| _version_ | 1818208634004307968 |
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| author | Yufen Wang Xianyang Xu Huiting Xue Dejian Zhang Guanhua Li |
| author_facet | Yufen Wang Xianyang Xu Huiting Xue Dejian Zhang Guanhua Li |
| author_sort | Yufen Wang |
| collection | DOAJ |
| description | Abstract Background Tissue heterogeneity significantly influences the overall saccharification efficiency of plant biomass. However, the mechanisms of specific organ or tissue recalcitrance to enzymatic deconstruction are generally complicated and unclear. A multidimensional analysis of the anatomical fraction from 12 corn cultivars was conducted to understand the essence of recalcitrance. Results The results showed that leaf, leaf sheath, stem pith and stem rind of corn straw exhibited remarkable heterogeneity in chemical composition, physical structure and cell type, which resulted in the different saccharification ratio of cellulose. The high saccharification ratio ranging from 21.47 to 38.96% was in stem pith, whereas the low saccharification ratio ranging from 17.1 to 27.43% was in leaf sheath. High values of lignin, hemicelluloses, degree of polymerization and crystallinity index were critical for the increased recalcitrance, while high value of neutral detergent soluble and pore size generated weak recalcitrance. Interestingly, pore traits of cell wall, especial for microcosmic interface structure, seemed to be a crucial factor that correlated to cellulase adsorption and further affected saccharification. Conclusions Highly heterogeneity in cell wall traits influenced the overall saccharification efficiency of biomass. Furthermore, the holistic outlook of cell wall interface was indispensable to understand the recalcitrance and promote the biomass conversion. Graphic abstract |
| first_indexed | 2024-12-12T04:47:56Z |
| format | Article |
| id | doaj.art-96b60cf1320046f48ad4c194c3b0cf26 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T04:47:56Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-96b60cf1320046f48ad4c194c3b0cf262022-12-22T00:37:35ZengBMCBiotechnology for Biofuels1754-68342021-10-0114111010.1186/s13068-021-02047-0Physical–chemical properties of cell wall interface significantly correlated to the complex recalcitrance of corn strawYufen Wang0Xianyang Xu1Huiting Xue2Dejian Zhang3Guanhua Li4Key Laboratory of Herbage and Endemic Crop Biotechnology, School of Life Sciences, Inner Mongolia UniversityKey Laboratory of Herbage and Endemic Crop Biotechnology, School of Life Sciences, Inner Mongolia UniversityCollege of Basic Medicine, Inner Mongolia Medical UniversityKey Laboratory of Herbage and Endemic Crop Biotechnology, School of Life Sciences, Inner Mongolia UniversityKey Laboratory of Herbage and Endemic Crop Biotechnology, School of Life Sciences, Inner Mongolia UniversityAbstract Background Tissue heterogeneity significantly influences the overall saccharification efficiency of plant biomass. However, the mechanisms of specific organ or tissue recalcitrance to enzymatic deconstruction are generally complicated and unclear. A multidimensional analysis of the anatomical fraction from 12 corn cultivars was conducted to understand the essence of recalcitrance. Results The results showed that leaf, leaf sheath, stem pith and stem rind of corn straw exhibited remarkable heterogeneity in chemical composition, physical structure and cell type, which resulted in the different saccharification ratio of cellulose. The high saccharification ratio ranging from 21.47 to 38.96% was in stem pith, whereas the low saccharification ratio ranging from 17.1 to 27.43% was in leaf sheath. High values of lignin, hemicelluloses, degree of polymerization and crystallinity index were critical for the increased recalcitrance, while high value of neutral detergent soluble and pore size generated weak recalcitrance. Interestingly, pore traits of cell wall, especial for microcosmic interface structure, seemed to be a crucial factor that correlated to cellulase adsorption and further affected saccharification. Conclusions Highly heterogeneity in cell wall traits influenced the overall saccharification efficiency of biomass. Furthermore, the holistic outlook of cell wall interface was indispensable to understand the recalcitrance and promote the biomass conversion. Graphic abstracthttps://doi.org/10.1186/s13068-021-02047-0Recalcitrant barrierPoreEnzymatic hydrolysisCell wallCorn straw |
| spellingShingle | Yufen Wang Xianyang Xu Huiting Xue Dejian Zhang Guanhua Li Physical–chemical properties of cell wall interface significantly correlated to the complex recalcitrance of corn straw Biotechnology for Biofuels Recalcitrant barrier Pore Enzymatic hydrolysis Cell wall Corn straw |
| title | Physical–chemical properties of cell wall interface significantly correlated to the complex recalcitrance of corn straw |
| title_full | Physical–chemical properties of cell wall interface significantly correlated to the complex recalcitrance of corn straw |
| title_fullStr | Physical–chemical properties of cell wall interface significantly correlated to the complex recalcitrance of corn straw |
| title_full_unstemmed | Physical–chemical properties of cell wall interface significantly correlated to the complex recalcitrance of corn straw |
| title_short | Physical–chemical properties of cell wall interface significantly correlated to the complex recalcitrance of corn straw |
| title_sort | physical chemical properties of cell wall interface significantly correlated to the complex recalcitrance of corn straw |
| topic | Recalcitrant barrier Pore Enzymatic hydrolysis Cell wall Corn straw |
| url | https://doi.org/10.1186/s13068-021-02047-0 |
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