Comparison of Ziehl-Neelsen's stain, fluorescent stain with CBNAAT of sputum for the diagnosis of pulmonary tuberculosis
Introduction: Pulmonary tuberculosis remains one of the most dangerous communicable diseases. More than two billion people are estimated to be infected with Mycobacterium tuberculosis. In 2017, approximately 10 million individuals became ill with tuberculosis,among them 2.79 million individuals were...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Journal of Dr. NTR University of Health Sciences |
| Subjects: | |
| Online Access: | http://www.jdrntruhs.org/article.asp?issn=2277-8632;year=2019;volume=8;issue=4;spage=238;epage=243;aulast=Padmaja |
| _version_ | 1828444720785784832 |
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| author | Goteti V Padmaja Kasukurthi Srujana Chittari Sadhana |
| author_facet | Goteti V Padmaja Kasukurthi Srujana Chittari Sadhana |
| author_sort | Goteti V Padmaja |
| collection | DOAJ |
| description | Introduction: Pulmonary tuberculosis remains one of the most dangerous communicable diseases. More than two billion people are estimated to be infected with Mycobacterium tuberculosis. In 2017, approximately 10 million individuals became ill with tuberculosis,among them 2.79 million individuals were active cases, 1.6 million died among which 0.3 million died with coexistent HIV infection. Prompt diagnosis of active tuberculosis facilitates timely therapeutic intervention and minimize the community transmission. As tuberculosis is a global problem, for eradication of the disease, early diagnosis, timely identification and improved detection is essential. The diagnosis of tuberculosis is challenging in cases of insufficient sputum and paucity of bacilli. With this background, this study was planned to compare Ziehl-Neelsen's stain, Fluorescent stain and CBNAAT (Cartridge based nucleic acid amplification test) as per RNTCP guidelines.
Material and Methods: A prospective interventional study was carried out using 188 sputum samples of suspected pulmonary tuberculosis. All 188 samples were subjected to three methods Ziehl-Neelsen's stain, fluorescent stain and CBNAAT. They were compared for sensitivity and specificity in terms of qualitative results. The data recorded was then analyzed statistically.
Results: In our study out of 188 cases 124 were males, 64 were females. Out of 188 samples, the smear positivity rate of ZN stain was 33.5%, fluorescent stain was 40.9%, and the positivity rate of CBNAAT was 51.1%.
Conclusion: CBNAAT is more sensitive and specific in detection of pulmonary TB. An additional feature of rifampicin resistance can also be detected by CBNAAT. |
| first_indexed | 2024-12-10T21:47:40Z |
| format | Article |
| id | doaj.art-96b6c5df667543bb9cb46010500eef94 |
| institution | Directory Open Access Journal |
| issn | 2277-8632 |
| language | English |
| last_indexed | 2024-12-10T21:47:40Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Dr. NTR University of Health Sciences |
| spelling | doaj.art-96b6c5df667543bb9cb46010500eef942022-12-22T01:32:19ZengWolters Kluwer Medknow PublicationsJournal of Dr. NTR University of Health Sciences2277-86322019-01-018423824310.4103/JDRNTRUHS.JDRNTRUHS_46_19Comparison of Ziehl-Neelsen's stain, fluorescent stain with CBNAAT of sputum for the diagnosis of pulmonary tuberculosisGoteti V PadmajaKasukurthi SrujanaChittari SadhanaIntroduction: Pulmonary tuberculosis remains one of the most dangerous communicable diseases. More than two billion people are estimated to be infected with Mycobacterium tuberculosis. In 2017, approximately 10 million individuals became ill with tuberculosis,among them 2.79 million individuals were active cases, 1.6 million died among which 0.3 million died with coexistent HIV infection. Prompt diagnosis of active tuberculosis facilitates timely therapeutic intervention and minimize the community transmission. As tuberculosis is a global problem, for eradication of the disease, early diagnosis, timely identification and improved detection is essential. The diagnosis of tuberculosis is challenging in cases of insufficient sputum and paucity of bacilli. With this background, this study was planned to compare Ziehl-Neelsen's stain, Fluorescent stain and CBNAAT (Cartridge based nucleic acid amplification test) as per RNTCP guidelines. Material and Methods: A prospective interventional study was carried out using 188 sputum samples of suspected pulmonary tuberculosis. All 188 samples were subjected to three methods Ziehl-Neelsen's stain, fluorescent stain and CBNAAT. They were compared for sensitivity and specificity in terms of qualitative results. The data recorded was then analyzed statistically. Results: In our study out of 188 cases 124 were males, 64 were females. Out of 188 samples, the smear positivity rate of ZN stain was 33.5%, fluorescent stain was 40.9%, and the positivity rate of CBNAAT was 51.1%. Conclusion: CBNAAT is more sensitive and specific in detection of pulmonary TB. An additional feature of rifampicin resistance can also be detected by CBNAAT.http://www.jdrntruhs.org/article.asp?issn=2277-8632;year=2019;volume=8;issue=4;spage=238;epage=243;aulast=Padmajaacid fast bacillicbnaatfluorescent stainpulmonary tuberculosisziehl-neelsen stain |
| spellingShingle | Goteti V Padmaja Kasukurthi Srujana Chittari Sadhana Comparison of Ziehl-Neelsen's stain, fluorescent stain with CBNAAT of sputum for the diagnosis of pulmonary tuberculosis Journal of Dr. NTR University of Health Sciences acid fast bacilli cbnaat fluorescent stain pulmonary tuberculosis ziehl-neelsen stain |
| title | Comparison of Ziehl-Neelsen's stain, fluorescent stain with CBNAAT of sputum for the diagnosis of pulmonary tuberculosis |
| title_full | Comparison of Ziehl-Neelsen's stain, fluorescent stain with CBNAAT of sputum for the diagnosis of pulmonary tuberculosis |
| title_fullStr | Comparison of Ziehl-Neelsen's stain, fluorescent stain with CBNAAT of sputum for the diagnosis of pulmonary tuberculosis |
| title_full_unstemmed | Comparison of Ziehl-Neelsen's stain, fluorescent stain with CBNAAT of sputum for the diagnosis of pulmonary tuberculosis |
| title_short | Comparison of Ziehl-Neelsen's stain, fluorescent stain with CBNAAT of sputum for the diagnosis of pulmonary tuberculosis |
| title_sort | comparison of ziehl neelsen s stain fluorescent stain with cbnaat of sputum for the diagnosis of pulmonary tuberculosis |
| topic | acid fast bacilli cbnaat fluorescent stain pulmonary tuberculosis ziehl-neelsen stain |
| url | http://www.jdrntruhs.org/article.asp?issn=2277-8632;year=2019;volume=8;issue=4;spage=238;epage=243;aulast=Padmaja |
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