Remineralising Potential of Marine Skeletal Species-Perna viridis Powder Extract on Human Teeth Enamel: An In-vitro Study
Introduction: Biomineralisation is a dynamic, complex, constant mechanism by which living organisms manage precipitations of inorganic nanocrystals within organic matrices to form unique hybrid biological tissues, for example, enamel, dentin, cementum, and bone. Realising the mechanism of minera...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2021-02-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://www.jcdr.net/articles/PDF/14482/46096_231220_46096_211220_46096_CE[Ra1]_F[SK]_PF1(AKA_KM)_PFA_NC(KM)_PN(KM).pdf |
| Summary: | Introduction: Biomineralisation is a dynamic, complex, constant
mechanism by which living organisms manage precipitations of
inorganic nanocrystals within organic matrices to form unique
hybrid biological tissues, for example, enamel, dentin, cementum,
and bone. Realising the mechanism of mineral deposition
is crucial for the progress of treatments for mineralisation
associated diseases and also for the innovation and development
of scaffolds.
Aim: To measure the remineralisation capacity of enamel surface
lesion using marine skeletal species-Perna viridis (mussel shell)
powder extract on human tooth enamel.
Materials and Methods: The in vitro quantitative
observational research study conducted from June 20th, 2020
to July 20th, 2020, included five freshly extracted un-erupted
third molars decoronated at Cemento-Enamel Junction (CEJ).
The coronal portion of single tooth was sectioned into 4
samples of enamel blocks in order to receive 20 blocks which
were subjected to demineralisation process and divided as:
Group A(Experimental group)-subsurface demineralisation
+ mussel shell powder extract and Group B (Control group)-
subsurface demineralisation + clinpro application being placed
in artificial saliva solution for thirty days. The specimens
were tested for X-ray fluorescence spectroscopy analysis,
microhardness testing and atomic analyses using Energy
dispersive X-ray spectroscopy. Statistical analysis was done
using unpaired t-test to check differences at the level of
p≤0.05 between groups which were considered as statistically
significant.
Results: X-ray fluorescence spectroscopy showed calcium
concentration in mussel shell of 95% and concentration of
Phosphate as 0.31%. Group B (Clinpro) presented statistically
significantly higher (p-value 0.028) potential in enhancing the
remineralisation than Group A: Mussel Shell Powder Extract
(MSPE). The results of atomic analyses exhibited that quantitative
amounts of Ca weight % and P weight % is statistically
insignificant between both the groups. Group B (Clinpro) showed
greatest ability in promoting remineralisation than Group A
(mussel shell extract).
Conclusion: Marine shells with 95% of calcium concentration
can remineralise enamel surface lesion. |
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| ISSN: | 2249-782X 0973-709X |