Molecular Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated in Poland
Shiga toxin-producing Escherichia coli (STEC) strains also called verotoxin-producing E. coli (VTEC) represent one of the most important groups of food-borne pathogens that can cause several human diseases such as hemorrhagic colitis (HC) and hemolytic – uremic syndrome (HUS) worldwide. Th...
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| Format: | Article |
| Language: | English |
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Sciendo
2016-08-01
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| Series: | Polish Journal of Microbiology |
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| Online Access: | https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/65/3/pdf/10.5604_17331331.1215601.pdf |
| _version_ | 1811220754967035904 |
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| author | Aleksandra Januszkiewicz Waldemar Rastawicki |
| author_facet | Aleksandra Januszkiewicz Waldemar Rastawicki |
| author_sort | Aleksandra Januszkiewicz |
| collection | DOAJ |
| description | Shiga toxin-producing Escherichia coli (STEC) strains also called verotoxin-producing E. coli (VTEC) represent one of the most important groups of food-borne pathogens that can cause several human diseases such as hemorrhagic colitis (HC) and hemolytic – uremic syndrome (HUS) worldwide. The ability of STEC strains to cause disease is associated with the presence of wide range of identified and putativevirulence factors including those encoding Shiga toxin. In this study, we examined the distribution of various virulence determinants among STEC strains isolated in Poland from different sources. A total of 71 Shiga toxin-producing E. coli strains isolated from human, cattle and food over the years 1996–2010 were characterized by microarray and PCR detection of virulence genes. As stx1a subtype was present in all of the tested Shiga toxin 1 producing E. coli strains, a greater diversity of subtypes was found in the gene stx2, which occurred in five subtypes: stx2a, stx2b, stx2c, stx2d, stx2g. Among STEC O157 strains we observed conserved core set of 14 virulence factors, stable in bacteria genome at long intervals of time. There was one cattle STEC isolate which possessed verotoxin gene as well as sta1 gene encoded heat-stable enterotoxin STIa characteristic for enterotoxigenic E. coli. To the best of our knowledge, this is the first comprehensive analysis of virulence gene profiles identified in STEC strains isolated from human, cattle and food in Poland. The results obtained using microarrays technology confirmed high effectiveness of this method in determining STEC virulotypes which provides data suitable for molecular risk assessment of the potential virulence of this bacteria. |
| first_indexed | 2024-04-12T07:47:57Z |
| format | Article |
| id | doaj.art-96b884604cd649b8ac7553c9c99d46f2 |
| institution | Directory Open Access Journal |
| issn | 1733-1331 2544-4646 |
| language | English |
| last_indexed | 2024-04-12T07:47:57Z |
| publishDate | 2016-08-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj.art-96b884604cd649b8ac7553c9c99d46f22022-12-22T03:41:40ZengSciendoPolish Journal of Microbiology1733-13312544-46462016-08-0165310.5604/17331331.1215601Molecular Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated in PolandAleksandra Januszkiewicz0Waldemar Rastawicki1National Institute of Public Health – National Institute of Hygiene, Department of Bacteriology, Warsaw, PolandNational Institute of Public Health – National Institute of Hygiene, Department of Bacteriology, Warsaw, PolandShiga toxin-producing Escherichia coli (STEC) strains also called verotoxin-producing E. coli (VTEC) represent one of the most important groups of food-borne pathogens that can cause several human diseases such as hemorrhagic colitis (HC) and hemolytic – uremic syndrome (HUS) worldwide. The ability of STEC strains to cause disease is associated with the presence of wide range of identified and putativevirulence factors including those encoding Shiga toxin. In this study, we examined the distribution of various virulence determinants among STEC strains isolated in Poland from different sources. A total of 71 Shiga toxin-producing E. coli strains isolated from human, cattle and food over the years 1996–2010 were characterized by microarray and PCR detection of virulence genes. As stx1a subtype was present in all of the tested Shiga toxin 1 producing E. coli strains, a greater diversity of subtypes was found in the gene stx2, which occurred in five subtypes: stx2a, stx2b, stx2c, stx2d, stx2g. Among STEC O157 strains we observed conserved core set of 14 virulence factors, stable in bacteria genome at long intervals of time. There was one cattle STEC isolate which possessed verotoxin gene as well as sta1 gene encoded heat-stable enterotoxin STIa characteristic for enterotoxigenic E. coli. To the best of our knowledge, this is the first comprehensive analysis of virulence gene profiles identified in STEC strains isolated from human, cattle and food in Poland. The results obtained using microarrays technology confirmed high effectiveness of this method in determining STEC virulotypes which provides data suitable for molecular risk assessment of the potential virulence of this bacteria.https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/65/3/pdf/10.5604_17331331.1215601.pdfEscherichia colimicroarray detectionShiga toxin-producingvirulence gene |
| spellingShingle | Aleksandra Januszkiewicz Waldemar Rastawicki Molecular Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated in Poland Polish Journal of Microbiology Escherichia coli microarray detection Shiga toxin-producing virulence gene |
| title | Molecular Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated in Poland |
| title_full | Molecular Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated in Poland |
| title_fullStr | Molecular Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated in Poland |
| title_full_unstemmed | Molecular Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated in Poland |
| title_short | Molecular Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated in Poland |
| title_sort | molecular characterization of shiga toxin producing escherichia coli strains isolated in poland |
| topic | Escherichia coli microarray detection Shiga toxin-producing virulence gene |
| url | https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/65/3/pdf/10.5604_17331331.1215601.pdf |
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