Spatiotemporal auxin distribution in Arabidopsis tissues is regulated by anabolic and catabolic reactions under long-term ammonium stress
Abstract Background The plant hormone auxin is a major coordinator of plant growth and development in response to diverse environmental signals, including nutritional conditions. Sole ammonium (NH4 +) nutrition is one of the unique growth-suppressing conditions for plants. Therefore, the quest to un...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-12-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-021-03385-9 |
| _version_ | 1819009447339491328 |
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| author | Kacper Dziewit Aleš Pěnčík Katarzyna Dobrzyńska Ondřej Novák Bożena Szal Anna Podgórska |
| author_facet | Kacper Dziewit Aleš Pěnčík Katarzyna Dobrzyńska Ondřej Novák Bożena Szal Anna Podgórska |
| author_sort | Kacper Dziewit |
| collection | DOAJ |
| description | Abstract Background The plant hormone auxin is a major coordinator of plant growth and development in response to diverse environmental signals, including nutritional conditions. Sole ammonium (NH4 +) nutrition is one of the unique growth-suppressing conditions for plants. Therefore, the quest to understand NH4 +-mediated developmental defects led us to analyze auxin metabolism. Results Indole-3-acetic acid (IAA), the most predominant natural auxin, accumulates in the leaves and roots of mature Arabidopsis thaliana plants grown on NH4 +, but not in the root tips. We found changes at the expressional level in reactions leading to IAA biosynthesis and deactivation in different tissues. Finally, NH4 + nutrition would facilitate the formation of inactive oxidized IAA as the final product. Conclusions NH4 +-mediated accelerated auxin turnover rates implicate transient and local IAA peaks. A noticeable auxin pattern in tissues correlates with the developmental adaptations of the short and highly branched root system of NH4 +-grown plants. Therefore, the spatiotemporal distribution of auxin might be a root-shaping signal specific to adjust to NH4 +-stress conditions. |
| first_indexed | 2024-12-21T00:56:31Z |
| format | Article |
| id | doaj.art-96bb28c9b8aa47c4a1f40f1181c34a17 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-21T00:56:31Z |
| publishDate | 2021-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-96bb28c9b8aa47c4a1f40f1181c34a172022-12-21T19:21:18ZengBMCBMC Plant Biology1471-22292021-12-0121111310.1186/s12870-021-03385-9Spatiotemporal auxin distribution in Arabidopsis tissues is regulated by anabolic and catabolic reactions under long-term ammonium stressKacper Dziewit0Aleš Pěnčík1Katarzyna Dobrzyńska2Ondřej Novák3Bożena Szal4Anna Podgórska5Institute of Plant Bioenergetics, Faculty of Biology, University of WarsawLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, The Czech Academy of SciencesInstitute of Plant Bioenergetics, Faculty of Biology, University of WarsawLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, The Czech Academy of SciencesInstitute of Plant Bioenergetics, Faculty of Biology, University of WarsawInstitute of Plant Bioenergetics, Faculty of Biology, University of WarsawAbstract Background The plant hormone auxin is a major coordinator of plant growth and development in response to diverse environmental signals, including nutritional conditions. Sole ammonium (NH4 +) nutrition is one of the unique growth-suppressing conditions for plants. Therefore, the quest to understand NH4 +-mediated developmental defects led us to analyze auxin metabolism. Results Indole-3-acetic acid (IAA), the most predominant natural auxin, accumulates in the leaves and roots of mature Arabidopsis thaliana plants grown on NH4 +, but not in the root tips. We found changes at the expressional level in reactions leading to IAA biosynthesis and deactivation in different tissues. Finally, NH4 + nutrition would facilitate the formation of inactive oxidized IAA as the final product. Conclusions NH4 +-mediated accelerated auxin turnover rates implicate transient and local IAA peaks. A noticeable auxin pattern in tissues correlates with the developmental adaptations of the short and highly branched root system of NH4 +-grown plants. Therefore, the spatiotemporal distribution of auxin might be a root-shaping signal specific to adjust to NH4 +-stress conditions.https://doi.org/10.1186/s12870-021-03385-9Ammonium nutritionArabidopsis thalianaAuxin conjugationAuxin degradationAuxin synthesisRoot development |
| spellingShingle | Kacper Dziewit Aleš Pěnčík Katarzyna Dobrzyńska Ondřej Novák Bożena Szal Anna Podgórska Spatiotemporal auxin distribution in Arabidopsis tissues is regulated by anabolic and catabolic reactions under long-term ammonium stress BMC Plant Biology Ammonium nutrition Arabidopsis thaliana Auxin conjugation Auxin degradation Auxin synthesis Root development |
| title | Spatiotemporal auxin distribution in Arabidopsis tissues is regulated by anabolic and catabolic reactions under long-term ammonium stress |
| title_full | Spatiotemporal auxin distribution in Arabidopsis tissues is regulated by anabolic and catabolic reactions under long-term ammonium stress |
| title_fullStr | Spatiotemporal auxin distribution in Arabidopsis tissues is regulated by anabolic and catabolic reactions under long-term ammonium stress |
| title_full_unstemmed | Spatiotemporal auxin distribution in Arabidopsis tissues is regulated by anabolic and catabolic reactions under long-term ammonium stress |
| title_short | Spatiotemporal auxin distribution in Arabidopsis tissues is regulated by anabolic and catabolic reactions under long-term ammonium stress |
| title_sort | spatiotemporal auxin distribution in arabidopsis tissues is regulated by anabolic and catabolic reactions under long term ammonium stress |
| topic | Ammonium nutrition Arabidopsis thaliana Auxin conjugation Auxin degradation Auxin synthesis Root development |
| url | https://doi.org/10.1186/s12870-021-03385-9 |
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