Antimicrobial Activity of a Phage Mixture and a Lactic Acid Bacterium against <i>Staphylococcus</i> <i>aureus</i> from Bovine Mastitis
The antimicrobial activity of a phage mixture and a lactic acid bacterium against <i>Staphylococcus aureus</i> isolates from bovine origin was investigated in vitro with regard to possible applications in the therapy of udder inflammation (mastitis) caused by bacterial infections. The &l...
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| Format: | Article |
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MDPI AG
2020-03-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/7/1/31 |
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| author | Isabel Titze Volker Krömker |
| author_facet | Isabel Titze Volker Krömker |
| author_sort | Isabel Titze |
| collection | DOAJ |
| description | The antimicrobial activity of a phage mixture and a lactic acid bacterium against <i>Staphylococcus aureus</i> isolates from bovine origin was investigated in vitro with regard to possible applications in the therapy of udder inflammation (mastitis) caused by bacterial infections. The <i>S. aureus</i> isolates used for inoculation derived from quarter foremilk samples of mastitis cases. For the examination of the antimicrobial activity, the reduction of the <i>S. aureus</i> germ density was determined [log<sub>10</sub> cfu/mL]. The phage mixture consisted of the three obligatory lytic and <i>S. aureus</i>-specific phages STA1.ST29, EB1.ST11 and EB1.ST27 (1:1:1). The selected <i>Lactobacillus plantarum</i> strain with proven antimicrobial properties and the phage mixture were tested against <i>S. aureus</i> in milk, both alone and in combination. The application of the lactic acid bacterium showed only a low reduction ability for a 24 h incubation period. The bacteriophage mixture as well as its combination with the lactic acid bacterium showed high antimicrobial activity against <i>S. aureus</i> for a 24 h incubation period at 37 °C, with only the phage mixture showing significance. |
| first_indexed | 2024-12-13T18:53:19Z |
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| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-12-13T18:53:19Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
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| series | Veterinary Sciences |
| spelling | doaj.art-96bc0618421049e09e609b610dfb31792022-12-21T23:34:53ZengMDPI AGVeterinary Sciences2306-73812020-03-01713110.3390/vetsci7010031vetsci7010031Antimicrobial Activity of a Phage Mixture and a Lactic Acid Bacterium against <i>Staphylococcus</i> <i>aureus</i> from Bovine MastitisIsabel Titze0Volker Krömker1Department of Bioprocess Engineering and Microbiology, Hannover University of Applied Sciences and Arts, D-30453 Hannover, GermanyDepartment of Bioprocess Engineering and Microbiology, Hannover University of Applied Sciences and Arts, D-30453 Hannover, GermanyThe antimicrobial activity of a phage mixture and a lactic acid bacterium against <i>Staphylococcus aureus</i> isolates from bovine origin was investigated in vitro with regard to possible applications in the therapy of udder inflammation (mastitis) caused by bacterial infections. The <i>S. aureus</i> isolates used for inoculation derived from quarter foremilk samples of mastitis cases. For the examination of the antimicrobial activity, the reduction of the <i>S. aureus</i> germ density was determined [log<sub>10</sub> cfu/mL]. The phage mixture consisted of the three obligatory lytic and <i>S. aureus</i>-specific phages STA1.ST29, EB1.ST11 and EB1.ST27 (1:1:1). The selected <i>Lactobacillus plantarum</i> strain with proven antimicrobial properties and the phage mixture were tested against <i>S. aureus</i> in milk, both alone and in combination. The application of the lactic acid bacterium showed only a low reduction ability for a 24 h incubation period. The bacteriophage mixture as well as its combination with the lactic acid bacterium showed high antimicrobial activity against <i>S. aureus</i> for a 24 h incubation period at 37 °C, with only the phage mixture showing significance.https://www.mdpi.com/2306-7381/7/1/31<i>staphylococcus aureus</i>bacteriophage mixturephage therapylytic phagelactic acid bacteriumdairybovine mastitis |
| spellingShingle | Isabel Titze Volker Krömker Antimicrobial Activity of a Phage Mixture and a Lactic Acid Bacterium against <i>Staphylococcus</i> <i>aureus</i> from Bovine Mastitis Veterinary Sciences <i>staphylococcus aureus</i> bacteriophage mixture phage therapy lytic phage lactic acid bacterium dairy bovine mastitis |
| title | Antimicrobial Activity of a Phage Mixture and a Lactic Acid Bacterium against <i>Staphylococcus</i> <i>aureus</i> from Bovine Mastitis |
| title_full | Antimicrobial Activity of a Phage Mixture and a Lactic Acid Bacterium against <i>Staphylococcus</i> <i>aureus</i> from Bovine Mastitis |
| title_fullStr | Antimicrobial Activity of a Phage Mixture and a Lactic Acid Bacterium against <i>Staphylococcus</i> <i>aureus</i> from Bovine Mastitis |
| title_full_unstemmed | Antimicrobial Activity of a Phage Mixture and a Lactic Acid Bacterium against <i>Staphylococcus</i> <i>aureus</i> from Bovine Mastitis |
| title_short | Antimicrobial Activity of a Phage Mixture and a Lactic Acid Bacterium against <i>Staphylococcus</i> <i>aureus</i> from Bovine Mastitis |
| title_sort | antimicrobial activity of a phage mixture and a lactic acid bacterium against i staphylococcus i i aureus i from bovine mastitis |
| topic | <i>staphylococcus aureus</i> bacteriophage mixture phage therapy lytic phage lactic acid bacterium dairy bovine mastitis |
| url | https://www.mdpi.com/2306-7381/7/1/31 |
| work_keys_str_mv | AT isabeltitze antimicrobialactivityofaphagemixtureandalacticacidbacteriumagainstistaphylococcusiiaureusifrombovinemastitis AT volkerkromker antimicrobialactivityofaphagemixtureandalacticacidbacteriumagainstistaphylococcusiiaureusifrombovinemastitis |