MiGut: A scalable in vitro platform for simulating the human gut microbiome—Development, validation and simulation of antibiotic‐induced dysbiosis
Abstract In vitro models of the human colon have been used extensively in understanding the human gut microbiome (GM) and evaluating how internal and external factors affect the residing bacterial populations. Such models have been shown to be highly predictive of in vivo outcomes and have a number...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-06-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.14259 |
| _version_ | 1797818580889239552 |
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| author | William A. Davis Birch Ines B. Moura Duncan J. Ewin Mark H. Wilcox Anthony M. Buckley Peter R. Culmer Nikil Kapur |
| author_facet | William A. Davis Birch Ines B. Moura Duncan J. Ewin Mark H. Wilcox Anthony M. Buckley Peter R. Culmer Nikil Kapur |
| author_sort | William A. Davis Birch |
| collection | DOAJ |
| description | Abstract In vitro models of the human colon have been used extensively in understanding the human gut microbiome (GM) and evaluating how internal and external factors affect the residing bacterial populations. Such models have been shown to be highly predictive of in vivo outcomes and have a number of advantages over animal models. The complexity required by in vitro models to closely mimic the physiology of the colon poses practical limits on their scalability. The scalable Mini Gut (MiGut) platform presented in this paper allows considerable expansion of model replicates and enables complex study design, without compromising on in vivo reflectiveness as is often the case with other model systems. MiGut has been benchmarked against a validated gut model in a demanding 9‐week study. MiGut showed excellent repeatability between model replicates and results were consistent with those of the benchmark system. The novel technology presented in this paper makes it conceivable that tens of models could be run simultaneously, allowing complex microbiome‐xenobiotic interactions to be explored in far greater detail, with minimal added resources or complexity. This platform expands the capacity to generate clinically relevant data to support our understanding of the cause‐effect relationships that govern the GM. |
| first_indexed | 2024-03-13T09:10:06Z |
| format | Article |
| id | doaj.art-96bcc92db8c84323b148abb3b6b3c9d7 |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-03-13T09:10:06Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-96bcc92db8c84323b148abb3b6b3c9d72023-05-27T09:33:44ZengWileyMicrobial Biotechnology1751-79152023-06-011661312132410.1111/1751-7915.14259MiGut: A scalable in vitro platform for simulating the human gut microbiome—Development, validation and simulation of antibiotic‐induced dysbiosisWilliam A. Davis Birch0Ines B. Moura1Duncan J. Ewin2Mark H. Wilcox3Anthony M. Buckley4Peter R. Culmer5Nikil Kapur6School of Mechanical Engineering University of Leeds Woodhouse Lane Leeds LS2 9JT UKHealthcare‐Associated Infections Group Leeds Institute of Medical Research, Faculty of Medicine and Health, University of Leeds Leeds LS2 9JT UKHealthcare‐Associated Infections Group Leeds Institute of Medical Research, Faculty of Medicine and Health, University of Leeds Leeds LS2 9JT UKHealthcare‐Associated Infections Group Leeds Institute of Medical Research, Faculty of Medicine and Health, University of Leeds Leeds LS2 9JT UKHealthcare‐Associated Infections Group Leeds Institute of Medical Research, Faculty of Medicine and Health, University of Leeds Leeds LS2 9JT UKSchool of Mechanical Engineering University of Leeds Woodhouse Lane Leeds LS2 9JT UKSchool of Mechanical Engineering University of Leeds Woodhouse Lane Leeds LS2 9JT UKAbstract In vitro models of the human colon have been used extensively in understanding the human gut microbiome (GM) and evaluating how internal and external factors affect the residing bacterial populations. Such models have been shown to be highly predictive of in vivo outcomes and have a number of advantages over animal models. The complexity required by in vitro models to closely mimic the physiology of the colon poses practical limits on their scalability. The scalable Mini Gut (MiGut) platform presented in this paper allows considerable expansion of model replicates and enables complex study design, without compromising on in vivo reflectiveness as is often the case with other model systems. MiGut has been benchmarked against a validated gut model in a demanding 9‐week study. MiGut showed excellent repeatability between model replicates and results were consistent with those of the benchmark system. The novel technology presented in this paper makes it conceivable that tens of models could be run simultaneously, allowing complex microbiome‐xenobiotic interactions to be explored in far greater detail, with minimal added resources or complexity. This platform expands the capacity to generate clinically relevant data to support our understanding of the cause‐effect relationships that govern the GM.https://doi.org/10.1111/1751-7915.14259 |
| spellingShingle | William A. Davis Birch Ines B. Moura Duncan J. Ewin Mark H. Wilcox Anthony M. Buckley Peter R. Culmer Nikil Kapur MiGut: A scalable in vitro platform for simulating the human gut microbiome—Development, validation and simulation of antibiotic‐induced dysbiosis Microbial Biotechnology |
| title | MiGut: A scalable in vitro platform for simulating the human gut microbiome—Development, validation and simulation of antibiotic‐induced dysbiosis |
| title_full | MiGut: A scalable in vitro platform for simulating the human gut microbiome—Development, validation and simulation of antibiotic‐induced dysbiosis |
| title_fullStr | MiGut: A scalable in vitro platform for simulating the human gut microbiome—Development, validation and simulation of antibiotic‐induced dysbiosis |
| title_full_unstemmed | MiGut: A scalable in vitro platform for simulating the human gut microbiome—Development, validation and simulation of antibiotic‐induced dysbiosis |
| title_short | MiGut: A scalable in vitro platform for simulating the human gut microbiome—Development, validation and simulation of antibiotic‐induced dysbiosis |
| title_sort | migut a scalable in vitro platform for simulating the human gut microbiome development validation and simulation of antibiotic induced dysbiosis |
| url | https://doi.org/10.1111/1751-7915.14259 |
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