Similar replicative fitness is shared by the subtype B and unique BF recombinant HIV-1 isolates that dominate the epidemic in Argentina.
The HIV-1 epidemic in South America is dominated by pure subtypes (mostly B and C) and more than 7 BF and BC recombinant forms. In Argentina, circulating recombinant forms (CRFs) comprised of subtypes B and F make up more than 50% of HIV infections. For this study, 28 HIV-1 primary isolates were obt...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3984079?pdf=render |
| _version_ | 1819261980773449728 |
|---|---|
| author | Andrea E Rubio Awet Abraha Crystal A Carpenter Ryan M Troyer Ángel L Reyes-Rodríguez Horacio Salomon Eric J Arts Denis M Tebit |
| author_facet | Andrea E Rubio Awet Abraha Crystal A Carpenter Ryan M Troyer Ángel L Reyes-Rodríguez Horacio Salomon Eric J Arts Denis M Tebit |
| author_sort | Andrea E Rubio |
| collection | DOAJ |
| description | The HIV-1 epidemic in South America is dominated by pure subtypes (mostly B and C) and more than 7 BF and BC recombinant forms. In Argentina, circulating recombinant forms (CRFs) comprised of subtypes B and F make up more than 50% of HIV infections. For this study, 28 HIV-1 primary isolates were obtained from patients in Buenos Aires, Argentina and initially classified into subtype B (n = 9, 32.1%), C (n = 1, 3.6%), and CRFs (n = 18, 64.3%) using partial pol and vpu-env sequences, which proved to be inconsistent and inaccurate for these phylogenetic analyses. Near full length genome sequences of these primary HIV-1 isolates revealed that nearly all intersubtype BF recombination sites were unique and countered previous "CRF" B/F classifications. The majority of these Argentinean HIV-1 isolates were CCR5-using but 4 had a dual/mixed tropism as predicted by both phenotypic and genotypic assays. Comparison of the replicative fitness of these BF primary HIV-1 isolates to circulating B, F, and C HIV-1 using pairwise competitions in peripheral blood mononuclear cells (PBMCs) indicated a similarity in fitness of these BF recombinants to subtypes B and F HIV-1 (of the same co-receptor usage) whereas subtype C HIV-1 was significantly less fit than all as previously reported. These results suggest that the multitude of BF HIV-1 strains present within the Argentinean population do not appear to have gained replicative fitness following recent B and F recombination events. |
| first_indexed | 2024-12-23T19:50:25Z |
| format | Article |
| id | doaj.art-96bdc658477e44c8a2eb9136566d4344 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T19:50:25Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-96bdc658477e44c8a2eb9136566d43442022-12-21T17:33:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9208410.1371/journal.pone.0092084Similar replicative fitness is shared by the subtype B and unique BF recombinant HIV-1 isolates that dominate the epidemic in Argentina.Andrea E RubioAwet AbrahaCrystal A CarpenterRyan M TroyerÁngel L Reyes-RodríguezHoracio SalomonEric J ArtsDenis M TebitThe HIV-1 epidemic in South America is dominated by pure subtypes (mostly B and C) and more than 7 BF and BC recombinant forms. In Argentina, circulating recombinant forms (CRFs) comprised of subtypes B and F make up more than 50% of HIV infections. For this study, 28 HIV-1 primary isolates were obtained from patients in Buenos Aires, Argentina and initially classified into subtype B (n = 9, 32.1%), C (n = 1, 3.6%), and CRFs (n = 18, 64.3%) using partial pol and vpu-env sequences, which proved to be inconsistent and inaccurate for these phylogenetic analyses. Near full length genome sequences of these primary HIV-1 isolates revealed that nearly all intersubtype BF recombination sites were unique and countered previous "CRF" B/F classifications. The majority of these Argentinean HIV-1 isolates were CCR5-using but 4 had a dual/mixed tropism as predicted by both phenotypic and genotypic assays. Comparison of the replicative fitness of these BF primary HIV-1 isolates to circulating B, F, and C HIV-1 using pairwise competitions in peripheral blood mononuclear cells (PBMCs) indicated a similarity in fitness of these BF recombinants to subtypes B and F HIV-1 (of the same co-receptor usage) whereas subtype C HIV-1 was significantly less fit than all as previously reported. These results suggest that the multitude of BF HIV-1 strains present within the Argentinean population do not appear to have gained replicative fitness following recent B and F recombination events.http://europepmc.org/articles/PMC3984079?pdf=render |
| spellingShingle | Andrea E Rubio Awet Abraha Crystal A Carpenter Ryan M Troyer Ángel L Reyes-Rodríguez Horacio Salomon Eric J Arts Denis M Tebit Similar replicative fitness is shared by the subtype B and unique BF recombinant HIV-1 isolates that dominate the epidemic in Argentina. PLoS ONE |
| title | Similar replicative fitness is shared by the subtype B and unique BF recombinant HIV-1 isolates that dominate the epidemic in Argentina. |
| title_full | Similar replicative fitness is shared by the subtype B and unique BF recombinant HIV-1 isolates that dominate the epidemic in Argentina. |
| title_fullStr | Similar replicative fitness is shared by the subtype B and unique BF recombinant HIV-1 isolates that dominate the epidemic in Argentina. |
| title_full_unstemmed | Similar replicative fitness is shared by the subtype B and unique BF recombinant HIV-1 isolates that dominate the epidemic in Argentina. |
| title_short | Similar replicative fitness is shared by the subtype B and unique BF recombinant HIV-1 isolates that dominate the epidemic in Argentina. |
| title_sort | similar replicative fitness is shared by the subtype b and unique bf recombinant hiv 1 isolates that dominate the epidemic in argentina |
| url | http://europepmc.org/articles/PMC3984079?pdf=render |
| work_keys_str_mv | AT andreaerubio similarreplicativefitnessissharedbythesubtypebanduniquebfrecombinanthiv1isolatesthatdominatetheepidemicinargentina AT awetabraha similarreplicativefitnessissharedbythesubtypebanduniquebfrecombinanthiv1isolatesthatdominatetheepidemicinargentina AT crystalacarpenter similarreplicativefitnessissharedbythesubtypebanduniquebfrecombinanthiv1isolatesthatdominatetheepidemicinargentina AT ryanmtroyer similarreplicativefitnessissharedbythesubtypebanduniquebfrecombinanthiv1isolatesthatdominatetheepidemicinargentina AT angellreyesrodriguez similarreplicativefitnessissharedbythesubtypebanduniquebfrecombinanthiv1isolatesthatdominatetheepidemicinargentina AT horaciosalomon similarreplicativefitnessissharedbythesubtypebanduniquebfrecombinanthiv1isolatesthatdominatetheepidemicinargentina AT ericjarts similarreplicativefitnessissharedbythesubtypebanduniquebfrecombinanthiv1isolatesthatdominatetheepidemicinargentina AT denismtebit similarreplicativefitnessissharedbythesubtypebanduniquebfrecombinanthiv1isolatesthatdominatetheepidemicinargentina |