Deinococcus radiodurans Toxin–Antitoxin MazEF-dr Mediates Cell Death in Response to DNA Damage Stress
Here we identified a functional MazEF-dr system in the exceptionally stress-resistant bacterium D. radiodurans. We showed that overexpression of the toxin MazF-dr inhibited the growth of Escherichia coli. The toxic effect of MazF-dr was due to its sequence-specific endoribonuclease activity on RNAs...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01427/full |
| _version_ | 1811313425846894592 |
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| author | Tao Li Yulan Weng Xiaoqiong Ma Bing Tian Shang Dai Ye Jin Mengjia Liu Jiulong Li Jiangliu Yu Yuejin Hua |
| author_facet | Tao Li Yulan Weng Xiaoqiong Ma Bing Tian Shang Dai Ye Jin Mengjia Liu Jiulong Li Jiangliu Yu Yuejin Hua |
| author_sort | Tao Li |
| collection | DOAJ |
| description | Here we identified a functional MazEF-dr system in the exceptionally stress-resistant bacterium D. radiodurans. We showed that overexpression of the toxin MazF-dr inhibited the growth of Escherichia coli. The toxic effect of MazF-dr was due to its sequence-specific endoribonuclease activity on RNAs containing a consensus 5′ACA3′, and it could be neutralized by MazE-dr. The MazF-dr showed a special cleavage preference for the nucleotide present before the ACA sequence with the order by U>A>G>C. MazEF-dr mediated the death of D. radiodurans cells under sub-lethal dose of stresses. The characteristics of programmed cell death (PCD) including membrane blebbing, loss of membrane integrity and cytoplasm condensation occurred in a fraction of the wild-type population at sub-lethal concentration of the DNA damaging agent mitomycin C (MMC); however, a MazEF-dr mutation relieved the cell death, suggesting that MazEF-dr mediated cell death through its endoribonuclease activity in response to DNA damage stress. The MazEF-dr-mediated cell death of a fraction of the population might serve as a survival strategy for the remaining population of D. radiodurans under DNA damage stress. |
| first_indexed | 2024-04-13T10:54:46Z |
| format | Article |
| id | doaj.art-96bf0774926841c99e984ed94648d4c9 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T10:54:46Z |
| publishDate | 2017-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-96bf0774926841c99e984ed94648d4c92022-12-22T02:49:34ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-07-01810.3389/fmicb.2017.01427279171Deinococcus radiodurans Toxin–Antitoxin MazEF-dr Mediates Cell Death in Response to DNA Damage StressTao Li0Yulan Weng1Xiaoqiong Ma2Bing Tian3Shang Dai4Ye Jin5Mengjia Liu6Jiulong Li7Jiangliu Yu8Yuejin Hua9Key Laboratory of Nuclear Agricultural Science of Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang UniversityHangzhou, ChinaKey Laboratory of Nuclear Agricultural Science of Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang UniversityHangzhou, ChinaCentral Laboratory, The First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou, ChinaKey Laboratory of Nuclear Agricultural Science of Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang UniversityHangzhou, ChinaKey Laboratory of Nuclear Agricultural Science of Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang UniversityHangzhou, ChinaKey Laboratory of Nuclear Agricultural Science of Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang UniversityHangzhou, ChinaKey Laboratory of Nuclear Agricultural Science of Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang UniversityHangzhou, ChinaKey Laboratory of Nuclear Agricultural Science of Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang UniversityHangzhou, ChinaKey Laboratory of Nuclear Agricultural Science of Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang UniversityHangzhou, ChinaKey Laboratory of Nuclear Agricultural Science of Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang UniversityHangzhou, ChinaHere we identified a functional MazEF-dr system in the exceptionally stress-resistant bacterium D. radiodurans. We showed that overexpression of the toxin MazF-dr inhibited the growth of Escherichia coli. The toxic effect of MazF-dr was due to its sequence-specific endoribonuclease activity on RNAs containing a consensus 5′ACA3′, and it could be neutralized by MazE-dr. The MazF-dr showed a special cleavage preference for the nucleotide present before the ACA sequence with the order by U>A>G>C. MazEF-dr mediated the death of D. radiodurans cells under sub-lethal dose of stresses. The characteristics of programmed cell death (PCD) including membrane blebbing, loss of membrane integrity and cytoplasm condensation occurred in a fraction of the wild-type population at sub-lethal concentration of the DNA damaging agent mitomycin C (MMC); however, a MazEF-dr mutation relieved the cell death, suggesting that MazEF-dr mediated cell death through its endoribonuclease activity in response to DNA damage stress. The MazEF-dr-mediated cell death of a fraction of the population might serve as a survival strategy for the remaining population of D. radiodurans under DNA damage stress.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01427/fulltoxin–antitoxin systemMazEFDeinococcus radioduranscell deathDNA damage stress |
| spellingShingle | Tao Li Yulan Weng Xiaoqiong Ma Bing Tian Shang Dai Ye Jin Mengjia Liu Jiulong Li Jiangliu Yu Yuejin Hua Deinococcus radiodurans Toxin–Antitoxin MazEF-dr Mediates Cell Death in Response to DNA Damage Stress Frontiers in Microbiology toxin–antitoxin system MazEF Deinococcus radiodurans cell death DNA damage stress |
| title | Deinococcus radiodurans Toxin–Antitoxin MazEF-dr Mediates Cell Death in Response to DNA Damage Stress |
| title_full | Deinococcus radiodurans Toxin–Antitoxin MazEF-dr Mediates Cell Death in Response to DNA Damage Stress |
| title_fullStr | Deinococcus radiodurans Toxin–Antitoxin MazEF-dr Mediates Cell Death in Response to DNA Damage Stress |
| title_full_unstemmed | Deinococcus radiodurans Toxin–Antitoxin MazEF-dr Mediates Cell Death in Response to DNA Damage Stress |
| title_short | Deinococcus radiodurans Toxin–Antitoxin MazEF-dr Mediates Cell Death in Response to DNA Damage Stress |
| title_sort | deinococcus radiodurans toxin antitoxin mazef dr mediates cell death in response to dna damage stress |
| topic | toxin–antitoxin system MazEF Deinococcus radiodurans cell death DNA damage stress |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01427/full |
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