Calcium dynamics and associated temporal patterns of milk constituents in early-lactation multiparous Holsteins
ABSTRACT: At the onset of lactation, calcium (Ca) homeostasis is challenged. For the transitioning dairy cow, inadequate responses to this challenge may result in subclinical hypocalcemia at some point in the postpartum period. It has been proposed that dynamics of blood Ca and the timing of subclin...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Journal of Dairy Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S002203022300245X |
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| author | J.A. Seminara K.R. Callero I.R. Frost R.M. Martinez H.A. McCray A.M. Reid C.R. Seely D.M. Barbano J.A.A. McArt |
| author_facet | J.A. Seminara K.R. Callero I.R. Frost R.M. Martinez H.A. McCray A.M. Reid C.R. Seely D.M. Barbano J.A.A. McArt |
| author_sort | J.A. Seminara |
| collection | DOAJ |
| description | ABSTRACT: At the onset of lactation, calcium (Ca) homeostasis is challenged. For the transitioning dairy cow, inadequate responses to this challenge may result in subclinical hypocalcemia at some point in the postpartum period. It has been proposed that dynamics of blood Ca and the timing of subclinical hypocalcemia allow cows to be classified into 4 Ca dynamic groups by assessing serum total Ca concentrations (tCa) at 1 and 4 days in milk (DIM). These differing dynamics are associated with different risks of adverse health events and suboptimal production. Our prospective cohort study aimed to characterize the temporal patterns of milk constituents in cows with differing Ca dynamics to investigate the potential of Fourier-transform infrared spectroscopic (FTIR) analysis of milk as a diagnostic tool for identifying cows with unfavorable Ca dynamics. We sampled the blood of 343 multiparous Holsteins on a single dairy in Cayuga County, New York, at 1 and 4 DIM and classified these cows into Ca dynamic groups using threshold concentrations of tCa (1 DIM: tCa <1.98 mmol/L; 4 DIM: tCa <2.22 mmol/L) derived from receiver operating characteristic curve analysis based on epidemiologically relevant health and production outcomes. We also collected proportional milk samples from each of these cows from 3 to 10 DIM for FTIR analysis of milk constituents. Through this analysis we estimated the milk constituent levels of anhydrous lactose (g/100 g of milk and g/milking), true protein (g/100 g of milk and g/milking), fat (g/100 g of milk and g/milking), milk urea nitrogen (mg/100 g of milk), fatty acid (FA) groups including de novo, mixed origin, and preformed FA measured in grams/100 g of milk, by relative percentage, and grams/milking, as well as energy-related metabolites including ketone bodies and milk-predicted blood nonesterified FA. Individual milk constituents were compared among groups at each time point and over the entire sample period using linear regression models. Overall, we found differences among the constituent profiles of Ca dynamic groups at approximately every time point and over the entire sample period. The 2 at-risk groups of cows did not differ from each other at more than one time point for any constituent, however prominent differences existed between the milk of normocalcemic cows and the milk of the other Ca dynamic groups with respect to FA. Over the entire sample period, lactose and protein yield (g/milking) were lower in the milk of at-risk cows than in the milk of the other Ca dynamic groups. In addition, milk yield per milking followed patterns consistent with previous Ca dynamic group research. Though our use of a single farm does limit the general applicability of these findings, our conclusions provide evidence that FTIR may be a useful method for discriminating between cows with different Ca dynamics at time points that may be relevant in the optimization of management or development of clinical intervention strategies. |
| first_indexed | 2024-03-11T22:28:01Z |
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| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-03-11T22:28:01Z |
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| series | Journal of Dairy Science |
| spelling | doaj.art-96bf4b2426624035b0f3da10b8f6cdd22023-09-24T05:13:34ZengElsevierJournal of Dairy Science0022-03022023-10-011061071177130Calcium dynamics and associated temporal patterns of milk constituents in early-lactation multiparous HolsteinsJ.A. Seminara0K.R. Callero1I.R. Frost2R.M. Martinez3H.A. McCray4A.M. Reid5C.R. Seely6D.M. Barbano7J.A.A. McArt8Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853College of Veterinary Medicine, Cornell University, Ithaca, NY 14853College of Arts and Sciences, Cornell University, Ithaca, NY 14853Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853Department of Food Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY 14853Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853; Corresponding authorABSTRACT: At the onset of lactation, calcium (Ca) homeostasis is challenged. For the transitioning dairy cow, inadequate responses to this challenge may result in subclinical hypocalcemia at some point in the postpartum period. It has been proposed that dynamics of blood Ca and the timing of subclinical hypocalcemia allow cows to be classified into 4 Ca dynamic groups by assessing serum total Ca concentrations (tCa) at 1 and 4 days in milk (DIM). These differing dynamics are associated with different risks of adverse health events and suboptimal production. Our prospective cohort study aimed to characterize the temporal patterns of milk constituents in cows with differing Ca dynamics to investigate the potential of Fourier-transform infrared spectroscopic (FTIR) analysis of milk as a diagnostic tool for identifying cows with unfavorable Ca dynamics. We sampled the blood of 343 multiparous Holsteins on a single dairy in Cayuga County, New York, at 1 and 4 DIM and classified these cows into Ca dynamic groups using threshold concentrations of tCa (1 DIM: tCa <1.98 mmol/L; 4 DIM: tCa <2.22 mmol/L) derived from receiver operating characteristic curve analysis based on epidemiologically relevant health and production outcomes. We also collected proportional milk samples from each of these cows from 3 to 10 DIM for FTIR analysis of milk constituents. Through this analysis we estimated the milk constituent levels of anhydrous lactose (g/100 g of milk and g/milking), true protein (g/100 g of milk and g/milking), fat (g/100 g of milk and g/milking), milk urea nitrogen (mg/100 g of milk), fatty acid (FA) groups including de novo, mixed origin, and preformed FA measured in grams/100 g of milk, by relative percentage, and grams/milking, as well as energy-related metabolites including ketone bodies and milk-predicted blood nonesterified FA. Individual milk constituents were compared among groups at each time point and over the entire sample period using linear regression models. Overall, we found differences among the constituent profiles of Ca dynamic groups at approximately every time point and over the entire sample period. The 2 at-risk groups of cows did not differ from each other at more than one time point for any constituent, however prominent differences existed between the milk of normocalcemic cows and the milk of the other Ca dynamic groups with respect to FA. Over the entire sample period, lactose and protein yield (g/milking) were lower in the milk of at-risk cows than in the milk of the other Ca dynamic groups. In addition, milk yield per milking followed patterns consistent with previous Ca dynamic group research. Though our use of a single farm does limit the general applicability of these findings, our conclusions provide evidence that FTIR may be a useful method for discriminating between cows with different Ca dynamics at time points that may be relevant in the optimization of management or development of clinical intervention strategies.http://www.sciencedirect.com/science/article/pii/S002203022300245Xsubclinical hypocalcemiaFourier-transform infrared spectroscopycalciumfatty acidsmilk |
| spellingShingle | J.A. Seminara K.R. Callero I.R. Frost R.M. Martinez H.A. McCray A.M. Reid C.R. Seely D.M. Barbano J.A.A. McArt Calcium dynamics and associated temporal patterns of milk constituents in early-lactation multiparous Holsteins Journal of Dairy Science subclinical hypocalcemia Fourier-transform infrared spectroscopy calcium fatty acids milk |
| title | Calcium dynamics and associated temporal patterns of milk constituents in early-lactation multiparous Holsteins |
| title_full | Calcium dynamics and associated temporal patterns of milk constituents in early-lactation multiparous Holsteins |
| title_fullStr | Calcium dynamics and associated temporal patterns of milk constituents in early-lactation multiparous Holsteins |
| title_full_unstemmed | Calcium dynamics and associated temporal patterns of milk constituents in early-lactation multiparous Holsteins |
| title_short | Calcium dynamics and associated temporal patterns of milk constituents in early-lactation multiparous Holsteins |
| title_sort | calcium dynamics and associated temporal patterns of milk constituents in early lactation multiparous holsteins |
| topic | subclinical hypocalcemia Fourier-transform infrared spectroscopy calcium fatty acids milk |
| url | http://www.sciencedirect.com/science/article/pii/S002203022300245X |
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