Integrative Transcriptomic and Phytohormonal Analyses Provide Insights into the Cold Injury Recovery Mechanisms of Tea Leaves
Tea plant is susceptible to low temperature, while the cold injury recovery mechanisms of tea leaves are still unclear. Windbreak has an effective and gradient range of protecting tea plants. Tea plants with increasing cold damage degree have varying recovery status accordingly, which are the ideal...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/20/2751 |
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| author | Zhi-Qi Ni Jing Jin Ying Ye Wen-Wen Luo Ya-Nan Zheng Zheng-Kun Tong Yi-Qing Lv Jian-Hui Ye Liang-Yu Wu |
| author_facet | Zhi-Qi Ni Jing Jin Ying Ye Wen-Wen Luo Ya-Nan Zheng Zheng-Kun Tong Yi-Qing Lv Jian-Hui Ye Liang-Yu Wu |
| author_sort | Zhi-Qi Ni |
| collection | DOAJ |
| description | Tea plant is susceptible to low temperature, while the cold injury recovery mechanisms of tea leaves are still unclear. Windbreak has an effective and gradient range of protecting tea plants. Tea plants with increasing cold damage degree have varying recovery status accordingly, which are the ideal objects for investigating the cold injury recovery mechanisms of tea leaves. Here, we investigated the transcriptome and phytohormone profiles of tea leaves with different cold injury degrees in recovery (adjacent to the windbreak), and the levels of chlorophylls, malondialdehyde, major phytohormones as well as the activities of peroxidase (POD) and superoxide dismutase (SOD) were also measured. The results showed the content of total chlorophylls and the activity of POD in mature tea leaves gradually decreased with the distance to windbreak, while SOD showed the opposite. The major phytohormones were highly accumulated in the moderately cold-injured tea leaves. The biosynthesis of abscisic acid (ABA) was enhanced in the moderate cold damaged tea leaves, suggesting that ABA plays an important role in the cold response and resistance of tea plants. The transcriptomic result showed that the samples in different rows were well discriminated, and the pathways of plant-pathogen interaction and flavonoid biosynthesis were enriched based on KEGG analysis. WRKY, GRAS and NAC were the top classes of transcription factors differentially expressed in the different cold-injured tea leaves. Thus, windbreak is effective to protect adjacent tea plants from cold wave, and phytohormones importantly participate in the cold injury recovery of tea leaves. |
| first_indexed | 2024-03-09T19:33:47Z |
| format | Article |
| id | doaj.art-96c18ed4b530469c954218941deaa31e |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T19:33:47Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-96c18ed4b530469c954218941deaa31e2023-11-24T02:04:46ZengMDPI AGPlants2223-77472022-10-011120275110.3390/plants11202751Integrative Transcriptomic and Phytohormonal Analyses Provide Insights into the Cold Injury Recovery Mechanisms of Tea LeavesZhi-Qi Ni0Jing Jin1Ying Ye2Wen-Wen Luo3Ya-Nan Zheng4Zheng-Kun Tong5Yi-Qing Lv6Jian-Hui Ye7Liang-Yu Wu8Tea Research Institute, Zijingang Campus, Zhejiang University, Hangzhou 310013, ChinaZhejiang Agricultural Technical Extension Center, 29 Fengqi East Road, Hangzhou 310020, ChinaTea Research Institute, Zijingang Campus, Zhejiang University, Hangzhou 310013, ChinaJinhua Department of Economic Specialty Technology Promotion, 828 Shuanglong South Road, Jinhua 321000, ChinaJinhua Department of Economic Specialty Technology Promotion, 828 Shuanglong South Road, Jinhua 321000, ChinaLanxi Chishan Lake Green Farm Co., Ltd., Lanxi 321100, ChinaTea Research Institute, Zijingang Campus, Zhejiang University, Hangzhou 310013, ChinaTea Research Institute, Zijingang Campus, Zhejiang University, Hangzhou 310013, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, 15 Shangxiadian Road, Fuzhou 350002, ChinaTea plant is susceptible to low temperature, while the cold injury recovery mechanisms of tea leaves are still unclear. Windbreak has an effective and gradient range of protecting tea plants. Tea plants with increasing cold damage degree have varying recovery status accordingly, which are the ideal objects for investigating the cold injury recovery mechanisms of tea leaves. Here, we investigated the transcriptome and phytohormone profiles of tea leaves with different cold injury degrees in recovery (adjacent to the windbreak), and the levels of chlorophylls, malondialdehyde, major phytohormones as well as the activities of peroxidase (POD) and superoxide dismutase (SOD) were also measured. The results showed the content of total chlorophylls and the activity of POD in mature tea leaves gradually decreased with the distance to windbreak, while SOD showed the opposite. The major phytohormones were highly accumulated in the moderately cold-injured tea leaves. The biosynthesis of abscisic acid (ABA) was enhanced in the moderate cold damaged tea leaves, suggesting that ABA plays an important role in the cold response and resistance of tea plants. The transcriptomic result showed that the samples in different rows were well discriminated, and the pathways of plant-pathogen interaction and flavonoid biosynthesis were enriched based on KEGG analysis. WRKY, GRAS and NAC were the top classes of transcription factors differentially expressed in the different cold-injured tea leaves. Thus, windbreak is effective to protect adjacent tea plants from cold wave, and phytohormones importantly participate in the cold injury recovery of tea leaves.https://www.mdpi.com/2223-7747/11/20/2751<i>Camellia sinensis</i>cold injury recoverywindbreakphytohormonetranscriptometranscription factor |
| spellingShingle | Zhi-Qi Ni Jing Jin Ying Ye Wen-Wen Luo Ya-Nan Zheng Zheng-Kun Tong Yi-Qing Lv Jian-Hui Ye Liang-Yu Wu Integrative Transcriptomic and Phytohormonal Analyses Provide Insights into the Cold Injury Recovery Mechanisms of Tea Leaves Plants <i>Camellia sinensis</i> cold injury recovery windbreak phytohormone transcriptome transcription factor |
| title | Integrative Transcriptomic and Phytohormonal Analyses Provide Insights into the Cold Injury Recovery Mechanisms of Tea Leaves |
| title_full | Integrative Transcriptomic and Phytohormonal Analyses Provide Insights into the Cold Injury Recovery Mechanisms of Tea Leaves |
| title_fullStr | Integrative Transcriptomic and Phytohormonal Analyses Provide Insights into the Cold Injury Recovery Mechanisms of Tea Leaves |
| title_full_unstemmed | Integrative Transcriptomic and Phytohormonal Analyses Provide Insights into the Cold Injury Recovery Mechanisms of Tea Leaves |
| title_short | Integrative Transcriptomic and Phytohormonal Analyses Provide Insights into the Cold Injury Recovery Mechanisms of Tea Leaves |
| title_sort | integrative transcriptomic and phytohormonal analyses provide insights into the cold injury recovery mechanisms of tea leaves |
| topic | <i>Camellia sinensis</i> cold injury recovery windbreak phytohormone transcriptome transcription factor |
| url | https://www.mdpi.com/2223-7747/11/20/2751 |
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